Results 131 to 140 of about 830,364 (264)

Targeting G protein–coupled receptor kinases to G protein–coupled receptors

Current Opinion in Endocrine and Metabolic Research, 2021
Sarah M. Sulon, Jeffrey L. Benovic
openaire   +2 more sources

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Structures of G-protein coupled receptor HCAR3 in complex with selective agonists reveal the basis for ligand recognition and selectivity. [PDF]

PLoS Biol
Ye F, Zhang Z, Zhang B, Li X, Deng J, Miao Q, Ning P, Chi Y, Chen G, Wu Z, Wang Q, Xu L, Gong N, Cheng B, Ma Z, Qian C, Zhu L, Pan X, Du Y.   +18 more
europepmc   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Investigating the Structural Basis of Diacetyl Recognition by the G-Protein-Coupled Receptor ODR-10 in <i>Caenorhabditis elegans</i>. [PDF]

J Phys Chem B
Di Rienzo L, Milanetti E, Folli V, Ruocco G.   +3 more
europepmc   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

Identification and localization of adhesion G protein-coupled receptor expression in the murine kidney. [PDF]

Am J Physiol Renal Physiol
Steichen H, Xue J, Zaidman NA.
europepmc   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Ciliary G-Protein Coupled Receptor Signaling in Polycystic Kidney Disease. [PDF]

Int J Mol Sci
Buqaileh R, Alshriem LA, AbouAlaiwi W.
europepmc   +1 more source