Results 91 to 100 of about 18,360,449 (307)

Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp. [PDF]

, 2012
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp).
Rüdiger, Stefan G. D., Stefan G D Rüdiger, Maurice, Madelon M., David P Minde, Sub Cellular Protein Chemistry, Stefan G. D. Rüdiger, Madelon M Maurice, Minde, David P., Cellular Protein Chemistry, David P. Minde, Madelon M. Maurice   +10 more
core   +3 more sources

RINGdb: An integrated database for G protein-coupled receptors and regulators of G protein signaling

BMC Genomics, 2006
Background Many marketed therapeutic agents have been developed to modulate the function of G protein-coupled receptors (GPCRs). The regulators of G-protein signaling (RGS proteins) are also being examined as potential drug targets.
Huang Hsien-Da, Wu Li-Cheng, Sun Wei-Hsin, Fang Yu-Ching, Juan Hsueh-Fen, Horng Jorng-Tzong   +5 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Development and evaluation of a speech-generating AAC mobile app for minimally verbal children with autism spectrum disorder in Mainland China

Molecular Autism, 2017
Background Mobile touchscreen devices are currently being used as speech-generating devices (SGDs) and have been shown to promote the communication skills, particularly the requesting skills of children with autism spectrum disorders (ASD) who have ...
Sainan An, Xiaoping Feng, Yue Dai, Hongli Bo, Xiuqing Wang, Mu Li, John Zhuohao Woo, Xingmei Liang, Cheng Guo, Charles Xingchao Liu, Liping Wei   +10 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Dimerization of G-Protein-Coupled Receptors

, 2016
The evolutionary trace (ET) method, a data mining approach for determining significant levels of amino acid conservation, has been applied to over 700 aligned G-protein-coupled receptor (GPCR) sequences.
Graham J. G. Upton (3012984), Trevor J. Howe (3010512), Christopher Higgs (2286679), Robert P. Bywater (2560129), Christopher R. Snell (2406547), Mark K. Dean (3012981), Richard E. Smith (3011007), Paul D. Scott (111064), Christopher A. Reynolds (473093)   +8 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Enhancing protein-protein docking by new approaches to protein flexibility and scoring of docking hypotheses [PDF]

, 2004
Zöllner FG. Enhancing protein-protein docking by new approaches to protein flexibility and scoring of docking hypotheses. Bielefeld (Germany): Bielefeld University; 2004.Für das Verständnis von biologischen Funktionen können Proteindockingverfahren ...
Frank G. Zöllner, Zöllner, Frank Gerrit   +1 more
core  

Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein

eLife, 2018
The adenosine A2A receptor (A2AR) is a prototypical G protein-coupled receptor (GPCR) that couples to the heterotrimeric G protein GS. Here, we determine the structure by electron cryo-microscopy (cryo-EM) of A2AR at pH 7.5 bound to the small molecule ...
Javier García-Nafría, Yang Lee, Xiaochen Bai, Byron Carpenter, Christopher G Tate   +4 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source