Results 111 to 120 of about 18,360,449 (307)

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors [PDF]

, 2011
Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E.coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs ...
Braun, Dieter, Shuguang Zhang, Baaske Philipp, Moran Jerabek-Willemsen, Geissler, Sandra, Duhr, Stefan, Duhr Stefan, Corin, Karolina A., Sandra Geissler, Wienken Christoph J., Christoph J. Wienken, Geissler Sandra, Jerabek-Willemsen, Moran, Christoph J Wienken, Zhang Shuguang, Stefan Duhr, Emily Brown, Wang Xiaoqiang, Philipp Baaske, Deepali B. Ravel, Brown, Emily, Song Junyao, Xiaoqiang Wang, Braun Dieter, Karolina Corin, Junyao Song, Wang, Xiaoqiang, Dieter Braun, Baaske, Philipp, Wienken, Christoph J., Ravel Deepali B., Corin, Karolina, Ravel, Deepali B., Brown Emily, Song, Junyao, Jerabek-Willemsen Moran, Corin Karolina, Zhang, Shuguang, Deepali B Ravel, Brown, Emily E., Ra Geissler   +40 more
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

A low resolution model for the interaction of G proteins with G protein-coupled receptors

, 1999
A model is presented for the interaction between G proteins and G protein-coupled receptors, the model is based on the fact that this interaction shows Little specificity and thus conserved parts of the G proteins have to interact with conserved parts of
Paiva, Antonio Cechelli de Mattos [UNIFESP], Vriend, Gerrit, Oliveira, Laerte [UNIFESP]   +2 more
core   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Bayesian sequence learning for predicting protein cleavage points [PDF]

, 2005
A challenging problem in data mining is the application of efficient techniques to automatically annotate the vast databases of biological sequence data.
Michael Mayo, Mayo, Michael
core   +1 more source

Biased antagonism of a series of bicyclic CXCR2 intracellular allosteric modulators

Frontiers in Pharmacology
Targeting the human chemokine receptor (CXCR2) holds significant potential in treating inflammatory diseases and cancer. In this study, we investigate the biased properties of previously reported CXCR2 antagonists (i.e., the MVH compounds).
Brent Van Bosstraeten, Katrijn Boon, Max Van Hoof, Wim Dehaen, Martyna Szpakowska, Andy Chevigné, Dominique Schols, Steven De Jonghe, Tom Van Loy   +8 more
doaj   +1 more source

Epigenetic reprogramming of lineage switching in cancer

FEBS Letters, EarlyView.
Cancer cells rarely commit to a single identity. Epigenetic mechanisms and tumor microenvironment cues push epithelial cells toward flexible, hybrid states that can shift into mesenchymal, neuroendocrine, or stem‐like fates, driving metastasis, drug resistance, and tumor heterogeneity. Targeting the epigenetic regulators behind these transitions, using
Ezgi Boyvatlı, Burcu Akman, Ezgi Bağırsakçı, Nese Atabey, Serap Erkek‐Ozhan   +4 more
wiley   +1 more source

Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor

Nature Communications, 2018
G protein-coupled receptors (GPCRs) activate and dissociate the G protein heterotrimer into Gα-GTP and Gβγ dimer, which facilitate distinct signalling events.
Sahil Gulati, Hui Jin, Ikuo Masuho, Tivadar Orban, Yuan Cai, Els Pardon, Kirill A. Martemyanov, Philip D. Kiser, Phoebe L. Stewart, Christopher P. Ford, Jan Steyaert, Krzysztof Palczewski   +11 more
doaj   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source