Results 81 to 90 of about 5,480,727 (338)

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

GPR35 as a novel therapeutic target

Frontiers in Endocrinology, 2011
G protein-coupled receptors (GPCRs) remain the best studied class of cell surface receptors and the most tractable family of proteins for novel small molecule drug discovery. Despite this, a considerable number of GPCRs remain poorly characterised and in
Amanda eMacKenzie, Jennifer eLappin, Debra eTaylor, Stuart eNicklin, Graeme eMilligan   +4 more
doaj   +1 more source

Structural basis of G protein–coupled receptor–G protein interactions [PDF]

Nature Chemical Biology, 2010
The interaction of G protein-coupled receptors (GPCRs) with heterotrimeric G proteins represents one of the most fundamental biological processes. However, the molecular architecture of the GPCR-G protein complex remains poorly defined. In the present study, we applied a comprehensive GPCR-G protein alpha subunit (Galpha) chemical cross-linking ...
Jürgen Wess, Jian Hua Li, Yan Wang, Xiaohong Zhang, Stefano Costanzi, Jianxin Hu, Joel Karpiak, John R. Lloyd   +7 more
openaire   +3 more sources

Targeting G protein-coupled receptor signalling by blocking G proteins [PDF]

Nature Reviews Drug Discovery, 2018
G protein-coupled receptors (GPCRs) are the largest class of drug targets, largely owing to their druggability, diversity and physiological efficacy. Many drugs selectively target specific subtypes of GPCRs, but high specificity for individual GPCRs may not be desirable in complex multifactorial disease states in which multiple receptors may be ...
Adrian Phillip Campbell, Alan V. Smrcka
openaire   +2 more sources

Single‐cell insights into the role of T cells in B‐cell malignancies

FEBS Letters, EarlyView.
Single‐cell technologies have transformed our understanding of T cell–tumor cell interactions in B‐cell malignancies, revealing new T‐cell subsets, functional states, and immune evasion mechanisms. This Review synthesizes these findings, highlighting the roles of T cells in pathogenesis, progression, and therapy response, and underscoring their ...
Laura Llaó‐Cid
wiley   +1 more source

Effects on Breathing of Agonists to μ-opioid or GABA\u3csub\u3eA\u3c/sub\u3e Receptors Dialyzed into the Ventral Respiratory Column of Awake and Sleeping Goats [PDF]

, 2017
Pulmonary ventilation (V̇I) in awake and sleeping goats does not change when antagonists to several excitatory G protein-coupled receptors are dialyzed unilaterally into the ventral respiratory column (VRC).
Burgraff, Nicholas J., Crumley, Emma, Forster, Hubert V., Hodges, Matthew R., Langer, Thomas M., III, Neumueller, Suzanne E., Pan, Lawrence, Talwar, Sawan   +7 more
core   +1 more source

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. [PDF]

, 2015
G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways.
Barty, Anton, Basu, Shibom, Boutet, Sébastien, Caffrey, Martin, Caro, Lydia N, Carragher, Bridget, Chapman, Henry N, Cherezov, Vadim, Coe, Jesse, Conrad, Chelsie E, de Waal, Parker W, Diederichs, Kay, Dong, Yuhui, Ernst, Oliver P, Fromme, Petra, Fromme, Raimund, Gao, Xiang, Gati, Cornelius, Griffin, Patrick R, Grotjohann, Ingo, Gu, Xin, Gurevich, Vsevolod V, Han, Gye Won, He, Yuanzheng, Howe, Nicole, Hubbell, Wayne L, Ishchenko, Andrii, James, Daniel, Jiang, Hualiang, Jiang, Yi, Kang, Yanyong, Katritch, Vsevolod, Ke, Jiyuan, Kupitz, Christopher, Lee, Regina J, Li, Dianfan, Li, Jun, Lisova, Stella, Liu, Haiguang, Liu, Wei, Ma, Jinming, Melcher, Karsten, Messerschmidt, Marc, Moeller, Arne, Pal, Kuntal, Pascal, Bruce D, Potter, Clinton S, Roy-Chowdhury, Shatabdi, Spence, John CH, Standfuss, Jörg, Stevens, Raymond C, Suino-Powell, Kelly M, Tan, MH Eileen, Tan, Minjia, Van Eps, Ned, Vishnivetskiy, Sergey A, Wang, Dingjie, Wang, Meitian, Weierstall, Uwe, West, Graham M, White, Thomas A, Williams, Garth J, Xu, H Eric, Xu, Qingping, Yang, Huaiyu, Yefanov, Oleksandr, Zatsepin, Nadia A, Zhang, Chenghai, Zhao, Yingming, Zheng, Zhong, Zhi, Xiaoyong, Zhou, X Edward   +71 more
core   +1 more source

Current applications of mini G proteins to study the structure and function of G protein-coupled receptors

AIMS Bioengineering, 2018
G protein-coupled receptors (GPCRs) regulate intracellular signalling pathways that contribute to virtually all aspects of cell function. Characterising GPCRs in each of their conformational states is key to understanding their mechanism of action, but ...
Byron Carpenter
doaj   +1 more source

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

FEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi, Yuka Hirosawa, Yusaku Sagisaka, Minami Hama, Satoshi Takeda, Jun A. Komano   +5 more
wiley   +1 more source

Sources of dietary protein and risk of hypertension in a general Dutch population [PDF]

, 2012
Evidence suggests a small beneficial effect of dietary protein on blood pressure (BP), especially for plant protein. We examined the relationship between several types of dietary protein (total, plant, animal, dairy, meat and grain) and the risk of ...
Altorf-van der Kuil, W., Boer, J.M.A., Engberink, M.F., Geleijnse, J.M., Verschuren, W.M.M.   +4 more
core   +2 more sources