Results 81 to 90 of about 35,623 (98)
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G-Proteins and Endothelial Responses
Journal of Vascular Research, 1990G-proteins are transducing proteins that couple a large number of membrane-bound receptors to a variety of intracellular effector systems. Pertussis toxin ADP-ribosylates certain G-proteins causing inhibition of their function. In porcine coronary arteries, pertussis toxin inhibited the endothelium-dependent relaxations evoked by alpha-2-adrenergic or ...
Flavahan, NA, Vanhoutte, PM
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Structural mechanism of G protein activation by G protein-coupled receptor
European Journal of Pharmacology, 2015G protein-coupled receptors (GPCRs) are a family of membrane receptors that regulate physiology and pathology of various organs. Consequently, about 40% of drugs in the market targets GPCRs. Heterotrimeric G proteins are composed of α, β, and γ subunits, and act as the key downstream signaling molecules of GPCRs.
Ka Young Chung +2 more
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When a G protein-coupled receptor does not couple to a G protein
Molecular BioSystems, 2007Classically, G protein-coupled receptors (GPCRs) relay signals by directly activating heterotrimeric guanine nucleotide-binding proteins (G proteins). Increasing evidence indicates that GPCRs may also signal through G protein-independent pathways. JAK/STATs, Src-family tyrosine kinases, GRKs/beta-arrestins, and PDZ domain-containing proteins have been ...
Yutong Sun +2 more
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Inherited Diseases Involving G Proteins and G Protein–Coupled Receptors
Annual Review of Medicine, 2004Heterotrimeric G proteins couple seven-transmembrane receptors for diverse extracellular signals to effectors that generate intracellular signals altering cell function. Mutations in the gene encoding the α subunit of the G protein–coupling receptors to stimulation of adenylyl cyclase cause developmental abnormalities of bone, as well as hormone ...
Lee S. Weinstein, Allen M. Spiegel
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A Conformational Trigger for Activation of a G Protein by a G Protein-Coupled Receptor
Biochemistry, 2003G protein-coupled receptors (GPCRs) are a family of seven transmembrane helical proteins that initiate a cellular response to an environmental signal. Once activated by an extracellular signal, GPCRs trigger the intracellular signal transduction cascade by activating a heterotrimeric G protein.
Arlene D. Albert, Philip L. Yeagle
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Chicken Anti-Protein G for the Detection of Small Amounts of Protein G
Hybridoma, 1993Streptococcal protein G, similar to staphylococcal protein A, is the Fc-binding proteins that seems best suited for affinity-purification of mouse, rat or human monoclonal antibodies. We have developed a sandwich-ELISA for monitoring of protein G leakage from such columns.
Anders Larsson, Tomas L. Lindahl
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Structure of G Protein–Coupled Receptors and G Proteins
Science's STKE, 2005This Teaching Resource provides lecture notes and slides for a class covering the structure and function of G protein–coupled receptors and is part of the course "Cell Signaling Systems: A Course for Graduate Students." The lecture begins with a discussion of the crystal structure of rhodopsin and G protein subunits and then proceeds to describe the ...
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Signaling by the G Class of G Proteins
Cellular Signalling, 1996The G12 class of G proteins are defined by the alpha-subunits of mammalian G12 and G13. Biochemical and mutational characterization of G alpha 12/13 have identified several novel signaling pathways regulated by these alpha-subunits. Studies with the constitutively activated mutants of G alpha 12 and G alpha 13 have indicated that they stimulate ...
Jonathan M. Dermott, N. Dhanasekaran
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G Proteins In Signal Transduction
Annual Review of Pharmacology and Toxicology, 1990The last five years have seen major advances in our understanding of the central and generalized role of G-proteins as transducers of receptor signals into effector responses as outlined in Scheme 1. Signal-transducing G pro teins, in contrast to other GTP-binding proteins, are heterotrimers formed of one GTP-binding (and hydrolyzing) a subunit, one ...
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Chimaeric protein A/protein G and protein G/alkaline phosphatase as reporter molecules
Journal of Immunological Methods, 1992The IgG binding domains of staphylococcal protein A and streptococcal protein G were expressed as a chimaera using the pGEX vector which has been advocated because its fusion proteins tend to be soluble and easily isolated on immobilised glutathione. This chimaera was soluble and abundant (yield = 18 mg/l of bacterial culture) and was tested by double ...
Shuhua Sun, Andrew M. Lew
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