Expression of each cistron in the gal operon can be regulated by transcription termination and generation of a galk-specific mRNA, mK2. [PDF]
J Bacteriol, 2014 ABSTRACT The gal operon of Escherichia coli has 4 cistrons, galE , galT , galK , and galM . In our previous report (H. J. Lee, H. J. Jeon, S. C. Ji, S. H. Yun, H. M. Lim, J. Mol. Biol.
Wang X +5 more
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DNA sequences in gal operon override transcription elongation blocks. [PDF]
J Mol Biol, 2008 The DNA loop that represses transcription from galactose (gal) promoters is infrequently formed in stationary-phase cells because the concentration of the loop architectural protein HU is significantly low at that state, resulting in expression of the operon in the absence of the gal inducer D-galactose.
Lewis DE +4 more
europepmc +7 more sources
Systematic characterization of a novel gal operon in Thermoanaerobacter tengcongensis [PDF]
Microbiology, 2009 On the basis of the Thermoanaerobacter tengcongensis genome, a novel type of gal operon was deduced. The gene expression and biochemical properties of this operon were further characterized.
Qian Zhong +9 more
semanticscholar +5 more sources
Structure of the galactokinase gene ofEscherichia coli, the last (?) gene of thegaloperon
Nucleic Acids Research, 1985 We present the nucleotide sequence of the galactokinase gene (galK) of Escherichia coli including its 5' and 3' flanking regions. This DNA sequence derives from the lambda gal8 transducing phage and is identical to the sequence present in the galK gene ...
Christine Debouck +9 more
semanticscholar +6 more sources
Cyclic AMP-dependent constitutive expression of gal operon: use of repressor titration to isolate operator mutations. [PDF]
Proceedings of the National Academy of Sciences, 1983 When the gal operator region is present in a multicopy plasmid it binds to all ("titrates") the gal repressor and "induces" the chromosomal gal operon. To make operator mutations (Oa) with reduced affinity toward the repressor, plasmid DNA was irradiated
Meher Irani +4 more
semanticscholar +7 more sources
The First Enzyme of the gal Operon in Inducible and Operator‐Constitutive Strains of Escherichia coli [PDF]
European Journal of Biochemistry, 1975 1. UDPgalactose 4-epimerase, the product of the first structural gene of the gal operon in Escherichia coli K-12, has been purified from strain HfrH and from its gal operator-constitutive mutant, HfrH 81-2. 2.
Roger G. Deeley +3 more
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Suppression of polarity of insertion mutations in the gal operon and N mutations in bacteriophage lambda [PDF]
Journal of Bacteriology, 1976 Bacterial mutations (psuA and psu) known for their ability to suppress the polarity on nonsense mutations are shown to suppress the polarity of certain insertion mutations in the gal operon. The short insertion, IS1 (800 nucleotide pairs), is about 15 to 50% suppressed, whereas longer insertions, IS2 (1,400 nucleotide pairs), and IS3 (1,200 nucleotide ...
Octavio Reyes López +2 more
semanticscholar +5 more sources
Reporter Gene-Based qRT-PCR Assay for Rho-Dependent Termination In Vivo [PDF]
Cells, 2023 In bacteria, the Rho protein mediates Rho-dependent termination (RDT) by identifying a non-specific cytosine-rich Rho utilization site on the newly synthesized RNA. As a result of RDT, downstream RNA transcription is reduced.
Monford Paul Abishek N +3 more
doaj +2 more sources
Base-pair opening and spermine binding—B-DNA features displayed in the crystal structure of a gal operon fragment: implications for protein-DNA recognition [PDF]
Nucleic Acids Research, 1995 A sequence that is represented frequently in functionally important sites involving protein-DNA interactions is GTG/CAC, suggesting that the trimer may play a role in regulatory processes.
Leslie W. Tari, A. S. Secco
openalex +2 more sources
The solution structure of a B-DNA undecamer comprising a portion of the specific target site for the cAMP receptor protein in the gal operon. Refinement on the basis of interproton distance data. [PDF]
EMBO Journal, 1985 G. Marius Clore, Angela M. Gronenborn
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