DNA sequences in gal operon override transcription elongation blocks. [PDF]
J Mol Biol, 2008 The DNA loop that represses transcription from galactose (gal) promoters is infrequently formed in stationary-phase cells because the concentration of the loop architectural protein HU is significantly low at that state, resulting in expression of the operon in the absence of the gal inducer D-galactose.
Lewis DE +4 more
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Rho-dependent termination and RNase E-mediated cleavage: dual pathways for RNA 3′ end processing in polycistronic mRNA [PDF]
Journal of Bacteriology“Pre-full-length” transcripts are produced at the end of the polycistronic galactose (gal) operon, 5′ galE-galT-galK-galM 3′, via Rho-dependent transcription termination (RDT) and -independent transcription termination.
Heung Jin Jeon +3 more
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Expression of each cistron in the gal operon can be regulated by transcription termination and generation of a galk-specific mRNA, mK2. [PDF]
J Bacteriol, 2014 Wang X +5 more
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Reporter Gene-Based qRT-PCR Assay for Rho-Dependent Termination In Vivo
Cells, 2023 In bacteria, the Rho protein mediates Rho-dependent termination (RDT) by identifying a non-specific cytosine-rich Rho utilization site on the newly synthesized RNA. As a result of RDT, downstream RNA transcription is reduced.
Monford Paul Abishek N +3 more
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The functional mutational landscape of the lacZ gene
iScience, 2023 Summary: The lacZ gene of Escherichia coli encodes β-galactosidase (β-gal), a lactose metabolism enzyme of the lactose operon. Previous chemical modification or site-directed mutagenesis experiments have identified 21 amino acids that are essential for β-
Marc A. Beal +5 more
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sRNA expedites polycistronic mRNA decay in Escherichia coli
Frontiers in Molecular Biosciences, 2023 In bacteria, most small RNA (sRNA) elicits RNase E-mediated target mRNA degradation by binding near the translation initiation site at the 5′ end of the target mRNA.
Heung Jin Jeon +5 more
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In vivo transcription dynamics of the galactose operon: a study on the promoter transition from P1 to P2 at onset of stationary phase. [PDF]
PLoS ONE, 2011 Quantitative analyses of the 5' end of gal transcripts indicate that transcription from the galactose operon P1 promoter is higher during cell division.
Sang Chun Ji +6 more
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Frontiers in Molecular Biosciences, 2022 GAL network in the yeast S. cerevisiae is one of the most well-characterized regulatory network. Expression of GAL genes is contingent on exposure to galactose, and an appropriate combination of the alleles of the regulatory genes GAL3, GAL1, GAL80, and ...
Rajeshkannan +2 more
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mBio, 2022 In Escherichia coli, transcription is coupled with translation. The polar gal operon is transcribed galE-galT-galK-galM; however, about 10% of transcription terminates at the end of galE because of Rho-dependent termination (RDT).
Heung Jin Jeon +3 more
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An Engineered Reporter Phage for the Fluorometric Detection of Escherichia coli in Ground Beef
Microorganisms, 2021 Despite enhanced sanitation implementations, foodborne bacterial pathogens still remain a major threat to public health and generate high costs for the food industry.
Anqi Chen +3 more
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