Results 111 to 120 of about 158,365 (141)
Oo and strong-polar mutations in the gal operon and insertions
Three λ dg phages carrying strong-polar mutations in the gal operon are denser than the corresponding phages carrying the wildtype gal operon or reversions of the mutations to the Gal + phenotype. The latter phages have the same density. It is concluded that these strong-polar mutations are insertions of DNA into the gal operon.
Elke Jordan+2 more
semanticscholar +5 more sources
Close vicinity of IS1 integration sites in the leader sequence of the gal operon of E. coli
Four insertions of IS1 in the leader sequence of the gal operon of E. coli have been analysed. Two of them occur at the same position, but in opposite orientations. The other two are inserted one nucleotide to one side and four nucleotides to the other side, respectively.
S. Kühn+2 more
semanticscholar +5 more sources
Insertion mutations in the control region of the Gal operon of E. coli
Galactose negative mutations are described which reduce the maximum expression of all three gal genes about 100-fold. The residual enzyme synthesis is not or only slightly inducible.
Heinz Saedler+4 more
semanticscholar +5 more sources
A control element within a structural gene: The gal operon of Escherichia coli
The gal operon of Escherichia coli is transcribed from two overlapping promoters, PG1 and PG2. Cyclic AMP and its receptor protein (CRP) modulate the two promoters in opposite directions by binding to a single cat locus. Both the promoters are negatively regulated by a single repressor, the product of the galR gene. An operator site, defined by several
Meher Irani+2 more
semanticscholar +5 more sources
Metabolism of messenger RNA from the gal operon of Escherichia coli
Abstract The size and metabolism of messenger RNA from the galactose (gal) operon of Escherichia coli has been studied. The three known structural genes of the operon are about 1100 nucleotide pairs each and code for an epimerase (E), transferase (T) and kinase (K) gene, in that order.
Daniel T. Achord, David Kennell
semanticscholar +5 more sources
mRNA distal to polar nonsense and insertion mutations in the gal operon of E. coli
mRNA of the galactose operon of E. coli was measured in wildtype E. coli and in gal operon amber and insertion mutants. The mRNA coded by the distal half of the operon is reduced in the mutants. This reduction is more pronounced in the insertion mutants than in the amber mutants. It was compared with the polar effects of the mutations on the enzymes of
Peter Starlinger+5 more
semanticscholar +5 more sources
Suppression of polarity of insertion mutations within the gal operon of E. coli
Phenotypic revertants of galOP::IS1 and galOP::IS2 mutations have been isolated after mutagenesis with nitrosoguanidine, they are probably caused by mutations in gene suA. The polarity suppressor mutations described in this study and a known mutation in gene suA isolated by D.
J Besemer, Maria Herpers
semanticscholar +5 more sources
Effects of dimethylsulfoxide on the E. coli gal operon and on bacteriophage lambda in vivo
Abstract Escherichia coli grown in a medium containing 10% (v/v) dimethylsulfoxide (DMSO) show constitutive synthesis of the enzymes of the gal operon, partial induction of λ prophage and extensive filament formation. The effect of DMSO is relatively specific for gal and λ because there is less than a 2 fold effect on the synthesis of several ...
Shigetada Nakanishi+3 more
semanticscholar +5 more sources