Results 161 to 170 of about 27,037 (204)

Construction and properties of hybrid plasmids carrying the E. coli gal operon

Gene, 1979
A family of hybrid plasmids carrying the entire gal operon of E. coli and designated pgal was constructed in vitro. In the case of pgal 1 (mol. wt. 16.4 Md), a fragment cut by Bam HI endonuclease from lambda gal phage DNA (lambda D-J-gal-att-int) was joined to pMB9 and cloned in the gal-strain of E.
T I, Tikchonenko, L Z, Faizullin, V N, Kalinin, N N, Granovsky, B S, Naroditsky   +4 more
openaire   +2 more sources

Non-random distribution of deletion endpoints in the gal operon of E. coli

Molecular and General Genetics MGG, 1974
The physical distances between endpoints of deletions in the gal operon of λdgal phages previously isolated and mapped by genetic methods have been measured by electron microscopy of heteroduplex DNA. Thus, the galT and galK sites previously mapped by genetic methods, may be assigned to 13 and 4 intervals, respectively, of known physical lengths.
Dietrich Pfeifer, Heinz‐Josef Hirsch, Dirk Bergmann, M.T. Hamlaoui   +3 more
openalex   +3 more sources

Close vicinity of IS1 integration sites in the leader sequence of the gal operon of E. coli

Molecular and General Genetics MGG, 1979
Four insertions of IS1 in the leader sequence of the gal operon of E. coli have been analysed. Two of them occur at the same position, but in opposite orientations. The other two are inserted one nucleotide to one side and four nucleotides to the other side, respectively.
S. Kühn, Hans‐Joachim Fritz, Peter Starlinger   +2 more
openalex   +3 more sources

Disco-ordinate expression of the Escherichia coli gal operon after prophage lambda induction

Journal of Molecular Biology, 1978
Abstract The three cistrons of the Escherichia coli gal operon are normally expressed co-ordinately. When the operon is transcribed from a prophage λ promoter, however, the expression is disco-ordinate. The product of the first cistron, galE , is found in much lower amounts than the products of the second and third cistrons, galT and galK . We
C, Merril, M, Gottesman, D, Court, S, Adhya   +3 more
openaire   +2 more sources

Insertion mutations in the control region of the Gal operon of E. coli

Molecular and General Genetics MGG, 1972
Galactose negative mutations are described which reduce the maximum expression of all three gal genes about 100-fold. The residual enzyme synthesis is not or only slightly inducible.
Heinz Saedler, J Besemer, B Kemper, Brigitte Rosenwirth, Peter Starlinger   +4 more
openalex   +3 more sources

A control element within a structural gene: The gal operon of Escherichia coli

Cell, 1983
The gal operon of Escherichia coli is transcribed from two overlapping promoters, PG1 and PG2. Cyclic AMP and its receptor protein (CRP) modulate the two promoters in opposite directions by binding to a single cat locus. Both the promoters are negatively regulated by a single repressor, the product of the galR gene. An operator site, defined by several
M H, Irani, L, Orosz, S, Adhya
openaire   +2 more sources

Effects of dimethylsulfoxide on the E. coli gal operon and on bacteriophage lambda in vivo

Cell, 1974
Abstract Escherichia coli grown in a medium containing 10% (v/v) dimethylsulfoxide (DMSO) show constitutive synthesis of the enzymes of the gal operon, partial induction of λ prophage and extensive filament formation. The effect of DMSO is relatively specific for gal and λ because there is less than a 2 fold effect on the synthesis of several ...
Shigetada Nakanishi, Sankar Adhya, Max E. Gottesman, Ira Pastan   +3 more
openalex   +3 more sources

Stimulation in trans of synthesis of E. coli gal operon enzymes by lambdoid phages during low catabolite repression

Molecular and General Genetics MGG, 1976
The infection of E. coli cells with different lambdoïd prophages triggers a stimulation of galactokinase synthesis when cells are grown in a medium giving rise to a mild catabolite repression (tryptone broth) with an inducer of the gal operon (fucose). These results show that during phage infection (or induction) some factor acting in trans is produced
E Petit-Koskas, G Contesse
openalex   +3 more sources

Dependence of Lactose Metabolism upon Mutarotase Encoded in the gal Operon in Escherichia coli

Journal of Molecular Biology, 1994
A new gene (galM) has been identified as the fourth cistron of the gal operon, encoding enzymes for the metabolism of galactose and lactose in Escherichia coli. Induction of the gal operon either from the gal promoters or from a neighboring prophage lambda promoter expresses the galM gene as well.
G G, Bouffard, K E, Rudd, S L, Adhya
openaire   +2 more sources

mRNA distal to polar nonsense and insertion mutations in the gal operon of E. coli

Molecular and General Genetics MGG, 1973
mRNA of the galactose operon of E. coli was measured in wildtype E. coli and in gal operon amber and insertion mutants. The mRNA coded by the distal half of the operon is reduced in the mutants. This reduction is more pronounced in the insertion mutants than in the amber mutants. It was compared with the polar effects of the mutations on the enzymes of
P, Starlinger, H, Saedler, B, Rak, E, Tillmann, P, Venkov, L, Waltschewa   +5 more
openaire   +2 more sources