Results 161 to 170 of about 172,673 (183)

Suppression of polarity of insertion mutations within the gal operon of E. coli

Molecular and General Genetics MGG, 1977
Phenotypic revertants of galOP::IS1 and galOP::IS2 mutations have been isolated after mutagenesis with nitrosoguanidine, they are probably caused by mutations in gene suA. The polarity suppressor mutations described in this study and a known mutation in gene suA isolated by D.
J Besemer, Maria Herpers
semanticscholar   +5 more sources

Effects of dimethylsulfoxide on the E. coli gal operon and on bacteriophage lambda in vivo

Cell, 1974
Abstract Escherichia coli grown in a medium containing 10% (v/v) dimethylsulfoxide (DMSO) show constitutive synthesis of the enzymes of the gal operon, partial induction of λ prophage and extensive filament formation. The effect of DMSO is relatively specific for gal and λ because there is less than a 2 fold effect on the synthesis of several ...
Shigetada Nakanishi, Sankar Adhya, Max E. Gottesman, Ira Pastan   +3 more
semanticscholar   +5 more sources

Non-random distribution of deletion endpoints in the gal operon of E. coli

Molecular and General Genetics MGG, 1974
The physical distances between endpoints of deletions in the gal operon of λdgal phages previously isolated and mapped by genetic methods have been measured by electron microscopy of heteroduplex DNA. Thus, the galT and galK sites previously mapped by genetic methods, may be assigned to 13 and 4 intervals, respectively, of known physical lengths.
Dietrich Pfeifer, Heinz‐Josef Hirsch, Dirk Bergmann, M.T. Hamlaoui   +3 more
semanticscholar   +5 more sources

Increased recombinant frequencies with an Amber mutation in the Gal-operon of E. coli

Molecular and General Genetics MGG, 1970
Two closely linked mutations, U20 and M87, in the deletion group V. of the transferase gene of the galactose operon in E. coli were crossed against a set of other mutations in the epimerase, transferase and kinase genes of the galactose operon by P1 transduction. U20 is an amber mutation.
Börries Kemper
semanticscholar   +5 more sources

Formation of lambda lysogens by IS2 recombination: gal operon-lambda pR promoter fusions

Virology, 1979
Abstract When Escherichia coli carrying an IS2 element in the gal operon are infected with lambda which also carry an IS2 element, some lysogens are formed by recombination between the two insertion elements. The resulting prophage are abnormally permuted. Whereas normal prophages are bracketed by two attachment sites, the abnormal prophages carry an
Oscar Reyes, Max E. Gottesman, Sankar Adhya   +2 more
semanticscholar   +5 more sources

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Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli.

Journal of Molecular Biology, 1994
A new gene (galM) has been identified as the fourth cistron of the gal operon, encoding enzymes for the metabolism of galactose and lactose in Escherichia coli.
G. Bouffard, K. Rudd, S. Adhya
semanticscholar   +4 more sources

Three-stage Regulation of the Amphibolic gal Operon: From Repressosome to GalR-free DNA

Journal of Molecular Biology, 2006
The gal operon of Escherichia coli is negatively regulated by the Gal repressosome, a higher order nucleoprotein complex containing a DNA loop that encompasses two gal promoters. In the repressosome structure, Gal repressor (GalR) dimers are bound to the two operator sites, flanking the promoter region, thus generating a DNA loop.
Szabolcs Semsey, Konstantin Virnik, Sankar Adhya   +2 more
semanticscholar   +4 more sources

Interactions between DNA-bound transcriptional regulators of the Escherichia coli gal operon.

Biochemistry, 1992
Regulation of the initiation of gene transcription from the gal operon of Escherichia coli is activated by the binding of CAP (catabolite activator protein) to a site centered at base pair -41.5 relative to the S1 start site of transcription. This operon
D. Dalma-Weiszhausz, M. Brenowitz
semanticscholar   +4 more sources

Disco-ordinate expression of the Escherichia coli gal operon after prophage lambda induction

Journal of Molecular Biology, 1978
Abstract The three cistrons of the Escherichia coli gal operon are normally expressed co-ordinately. When the operon is transcribed from a prophage λ promoter, however, the expression is disco-ordinate. The product of the first cistron, galE , is found in much lower amounts than the products of the second and third cistrons, galT and galK . We
Carl R. Merril, Max Gottesman, Don Court, Sankar Adhya   +3 more
semanticscholar   +5 more sources

Interactions between RNA polymerase and the positive and negative regulators of transcription at the Escherichia coli gal operon.

Biochemistry, 1996
The simultaneous binding of Gal repressor (GalR), catabolite activator protein (CAP or CRP), and RNA polymerase (RNAP) to the promoter region of the Escherichia coli gal operon has been analyzed thermodynamically, by quantitative DNase I "footprint ...
D. Dalma-Weiszhausz, M. Brenowitz
semanticscholar   +4 more sources