Results 11 to 20 of about 172,673 (183)
A mutation defining ultrainduction of the Escherichia coli gal operon [PDF]
Journal of Bacteriology, 1991 Tn10 insertion in the galS (ultrainduction factor) gene of Escherichia coli allows the gal operon to be constitutively expressed at a very high level, equal to that seen in a delta galR strain in the presence of an inducer. The insertion has been mapped by criss-cross Hfr matings and by marker rescue into Kohara phages at 46 min on the E.
Amit Golding +4 more
semanticscholar +6 more sources
Further inducibility of a constitutive system: ultrainduction of the gal operon [PDF]
Journal of Bacteriology, 1991 In wild-type Escherichia coli, expression of the gal operon is negatively regulated by the Gal repressor and is induced 10- to 15-fold when the repressor is inactivated by an inducer. In strains completely deleted for galR, the gene which encodes the Gal repressor, the operon is derepressed by only 10-fold without an inducer.
J P Tokeson, Susan Garges, Sankar Adhya
semanticscholar +5 more sources
Transcriptional Regulation of the Streptococcus mutans gal Operon by the GalR Repressor [PDF]
Journal of Bacteriology, 1998 ABSTRACT The galactose operon of Streptococcus mutans is transcriptionally regulated by a repressor protein (GalR) encoded by the galR gene, which is divergently oriented from the structural genes of the gal operon.
Dragana Ajdić, Joseph J. Ferretti
semanticscholar +5 more sources
Regulation of the gal Operon of Escherichia coli by the capR Gene
Journal of Biological Chemistry, 1972 Abstract We have examined in greater detail the derepression of uridine diphosphate galactose-4-epimerase synthesis in capR mutants of Escherichia coli first observed by Markovitz. (Markovitz, A. (1964) Proc. Nat. Acad. Sci. U. S. A. 51, 239–246). All three enzymes of the gal operon are derepressed from 2- to 4-fold by this mutation.
George A. Mackie, David B. Wilson
semanticscholar +5 more sources
Gene organization and structure of the Streptomyces lividans gal operon [PDF]
Journal of Bacteriology, 1988 We present the gene organization and DNA sequence of the Streptomyces lividans galactose utilization genes. Complementation of Escherichia coli galE, galT, or galK mutants and DNA sequence analysis were used to demonstrate that the galactose utilization genes are organized within an operon with the gene order galT, galE, and galK.
C W Adams +4 more
semanticscholar +5 more sources
Escherichia coli gal operon proteins made after prophage lambda induction [PDF]
Journal of Bacteriology, 1981 Expression of the EScherichia coli gal operon under the control of the prophage lambda promoter pL leads to gross discoordinacy of gal expression. Expression of the most promoter-distal cistron galK is much greater than expression of the promoter-proximal cistron galE.
C R Merril +2 more
semanticscholar +4 more sources
Isolation of a λd v Plasmid Carrying the Bacterial gal Operon [PDF]
Journal of Virology, 1974 A λ dvgal plasmid carrying genes for controlled plasmid replication from phage λ and the bacterial gal operon was isolated as a deletion mutant of phage λ gal q4, which carries the gal operon between λ genes P and
Douglas E. Berg +2 more
semanticscholar +6 more sources
72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli. [PDF]
The EMBO Journal, 1983 An active gene has been constructed which produces a chimera consisting of the N-terminal domain of the gal repressor and all but the first five residues of beta-galactosidase. Seventy two residues of gal repressor fused to beta-galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro.
B. von Wilcken‐Bergmann +3 more
semanticscholar +6 more sources
Transcriptional activation of the glycolytic las operon and catabolite repression of the gal operon in Lactococcus lactis are mediated by the catabolite control protein CcpA [PDF]
Molecular Microbiology, 1998 The Lactococcus lactis ccpA gene, encoding the global regulatory protein CcpA, was identified and characterized. Northern blot and primer extension analyses showed that the L. lactis ccpA gene is constitutively transcribed from a promoter that does not contain a cre sequence.
Evert J. Luesink +4 more
semanticscholar +7 more sources
In Vitro Transcription of the Gal Operon Requires Cyclic Adenosine Monophosphate and Cyclic Adenosine Monophosphate Receptor Protein [PDF]
Journal of Biological Chemistry, 1971 Abstract In a transcription system containing λpgal8 DNA as template and Escherichia coli RNA polymerase, the addition of cyclic adenosine monophosphate receptor protein (CRP) and 3',5'-cyclic AMP causes a 15-fold increase in gal mRNA synthesis. Gal mRNA was measured by hybridizing the [3H]RNA product to the separated strands of λpgal8 DNA after ...
S. Peter Nissley +4 more
semanticscholar +4 more sources