Results 11 to 20 of about 27,037 (204)
Escherichia coli gal operon proteins made after prophage lambda induction [PDF]
Journal of Bacteriology, 1981 Expression of the EScherichia coli gal operon under the control of the prophage lambda promoter pL leads to gross discoordinacy of gal expression. Expression of the most promoter-distal cistron galK is much greater than expression of the promoter-proximal cistron galE.
C R Merril +2 more
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Gene organization and structure of the Streptomyces lividans gal operon [PDF]
Journal of Bacteriology, 1988 We present the gene organization and DNA sequence of the Streptomyces lividans galactose utilization genes. Complementation of Escherichia coli galE, galT, or galK mutants and DNA sequence analysis were used to demonstrate that the galactose utilization genes are organized within an operon with the gene order galT, galE, and galK.
C W Adams +4 more
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Further inducibility of a constitutive system: ultrainduction of the gal operon [PDF]
Journal of Bacteriology, 1991 In wild-type Escherichia coli, expression of the gal operon is negatively regulated by the Gal repressor and is induced 10- to 15-fold when the repressor is inactivated by an inducer. In strains completely deleted for galR, the gene which encodes the Gal repressor, the operon is derepressed by only 10-fold without an inducer.
J P Tokeson, Susan Garges, Sankar Adhya
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Suppression of polarity in the gal operon by ultraviolet irradiation [PDF]
Journal of Bacteriology, 1980 The polarity of nonsense mutations in the galE gene of Escherichia coli can be suppressed by rho mutations (O. Reyes et al., J. Bacteriol. 126:1108-1112, 1976). We show here that this polarity can also be suppressed by ultraviolet irradiation. The effect is analogous to that already observed for polar nonsense mutations in phi X174 and S13 and suggests
Jan S. Fassler, Irwin Tessman
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Regulation of galactose operon at the gal operator-promoter region in Escherichia coli K-12 [PDF]
Journal of Bacteriology, 1975 The capR (lon) product controls expression of the gal operon independently of the galR repressor. Previously, mutations of the gal operon have been isolated that are semiconstitutive and alter response to the capR and/or capT product. Such mutants imply the existence of a distinct site in the operon that responds to capR (capT) control.
Shaofeng Hua, Alvin Markovitz
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72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli. [PDF]
The EMBO Journal, 1983 An active gene has been constructed which produces a chimera consisting of the N-terminal domain of the gal repressor and all but the first five residues of beta-galactosidase. Seventy two residues of gal repressor fused to beta-galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro.
B. von Wilcken‐Bergmann +3 more
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Suppression of polarity of insertion mutations in the gal operon and N mutations in bacteriophage lambda [PDF]
Journal of Bacteriology, 1976 Bacterial mutations (psuA and psu) known for their ability to suppress the polarity on nonsense mutations are shown to suppress the polarity of certain insertion mutations in the gal operon. The short insertion, IS1 (800 nucleotide pairs), is about 15 to 50% suppressed, whereas longer insertions, IS2 (1,400 nucleotide pairs), and IS3 (1,200 nucleotide ...
Octavio Reyes López +2 more
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A mutation defining ultrainduction of the Escherichia coli gal operon [PDF]
Journal of Bacteriology, 1991 Tn10 insertion in the galS (ultrainduction factor) gene of Escherichia coli allows the gal operon to be constitutively expressed at a very high level, equal to that seen in a delta galR strain in the presence of an inducer. The insertion has been mapped by criss-cross Hfr matings and by marker rescue into Kohara phages at 46 min on the E.
Amit Golding +4 more
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In Vitro Repression of the Transcription of gal Operon by Purified gal Repressor [PDF]
Proceedings of the National Academy of Sciences, 1973 We have studied the in vitro repression of gal mRNA synthesis by the gal repressor from Escherichia coli . By use of a four-step purification procedure involving chromatography on phosphocellulose, DEAE-cellulose, and an affinity resin, the
Shigetada Nakanishi +3 more
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Transcriptional Regulation of the Streptococcus mutans gal Operon by the GalR Repressor [PDF]
Journal of Bacteriology, 1998 ABSTRACT The galactose operon of Streptococcus mutans is transcriptionally regulated by a repressor protein (GalR) encoded by the galR gene, which is divergently oriented from the structural genes of the gal operon.
Dragana Ajdić, Joseph J. Ferretti
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