Isolation of a λd v Plasmid Carrying the Bacterial gal Operon [PDF]
Journal of Virology, 1974 A λ dvgal plasmid carrying genes for controlled plasmid replication from phage λ and the bacterial gal operon was isolated as a deletion mutant of phage λ gal q4, which carries the gal operon between λ genes P and
Douglas E. Berg +2 more
semanticscholar +6 more sources
Structure of the galactokinase gene ofEscherichia coli, the last (?) gene of thegaloperon
Nucleic Acids Research, 1985 We present the nucleotide sequence of the galactokinase gene (galK) of Escherichia coli including its 5' and 3' flanking regions. This DNA sequence derives from the lambda gal8 transducing phage and is identical to the sequence present in the galK gene ...
Christine Debouck +9 more
semanticscholar +6 more sources
Cyclic AMP-dependent constitutive expression of gal operon: use of repressor titration to isolate operator mutations. [PDF]
Proceedings of the National Academy of Sciences, 1983 When the gal operator region is present in a multicopy plasmid it binds to all ("titrates") the gal repressor and "induces" the chromosomal gal operon. To make operator mutations (Oa) with reduced affinity toward the repressor, plasmid DNA was irradiated
Meher Irani +4 more
semanticscholar +7 more sources
72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli. [PDF]
The EMBO Journal, 1983 An active gene has been constructed which produces a chimera consisting of the N-terminal domain of the gal repressor and all but the first five residues of beta-galactosidase. Seventy two residues of gal repressor fused to beta-galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro.
B. von Wilcken‐Bergmann +3 more
semanticscholar +6 more sources
In Vitro Transcription of the Gal Operon Requires Cyclic Adenosine Monophosphate and Cyclic Adenosine Monophosphate Receptor Protein [PDF]
Journal of Biological Chemistry, 1971 Abstract In a transcription system containing λpgal8 DNA as template and Escherichia coli RNA polymerase, the addition of cyclic adenosine monophosphate receptor protein (CRP) and 3',5'-cyclic AMP causes a 15-fold increase in gal mRNA synthesis. Gal mRNA was measured by hybridizing the [3H]RNA product to the separated strands of λpgal8 DNA after ...
S. Peter Nissley +4 more
semanticscholar +4 more sources
Transcriptional activation of the glycolytic las operon and catabolite repression of the gal operon in Lactococcus lactis are mediated by the catabolite control protein CcpA [PDF]
Molecular Microbiology, 1998 The Lactococcus lactis ccpA gene, encoding the global regulatory protein CcpA, was identified and characterized. Northern blot and primer extension analyses showed that the L. lactis ccpA gene is constitutively transcribed from a promoter that does not contain a cre sequence.
Evert J. Luesink +4 more
semanticscholar +7 more sources
The First Enzyme of the gal Operon in Inducible and Operator‐Constitutive Strains of Escherichia coli [PDF]
European Journal of Biochemistry, 1975 1. UDPgalactose 4-epimerase, the product of the first structural gene of the gal operon in Escherichia coli K-12, has been purified from strain HfrH and from its gal operator-constitutive mutant, HfrH 81-2. 2.
Roger G. Deeley +3 more
semanticscholar +6 more sources
Reporter Gene-Based qRT-PCR Assay for Rho-Dependent Termination In Vivo [PDF]
Cells, 2023 In bacteria, the Rho protein mediates Rho-dependent termination (RDT) by identifying a non-specific cytosine-rich Rho utilization site on the newly synthesized RNA. As a result of RDT, downstream RNA transcription is reduced.
Monford Paul Abishek N +3 more
doaj +2 more sources
Suppression of polarity in the gal operon by ultraviolet irradiation [PDF]
Journal of Bacteriology, 1980 The polarity of nonsense mutations in the galE gene of Escherichia coli can be suppressed by rho mutations (O. Reyes et al., J. Bacteriol. 126:1108-1112, 1976). We show here that this polarity can also be suppressed by ultraviolet irradiation. The effect is analogous to that already observed for polar nonsense mutations in phi X174 and S13 and suggests
Jan S. Fassler, Irwin Tessman
openalex +4 more sources
In Vitro Repression of the Transcription of gal Operon by Purified gal Repressor [PDF]
Proceedings of the National Academy of Sciences, 1973 We have studied the in vitro repression of gal mRNA synthesis by the gal repressor from Escherichia coli . By use of a four-step purification procedure involving chromatography on phosphocellulose, DEAE-cellulose, and an affinity resin, the
Shigetada Nakanishi +3 more
openalex +4 more sources