Results 21 to 30 of about 27,037 (204)

Isolation of a λd v Plasmid Carrying the Bacterial gal Operon [PDF]

open access: bronzeJournal of Virology, 1974
A λ dvgal plasmid carrying genes for controlled plasmid replication from phage λ and the bacterial gal operon was isolated as a deletion mutant of phage λ gal q4, which carries the gal operon between λ genes P and
Douglas E. Berg   +2 more
openalex   +4 more sources

In Vitro Transcription of the Gal Operon Requires Cyclic Adenosine Monophosphate and Cyclic Adenosine Monophosphate Receptor Protein

open access: hybridJournal of Biological Chemistry, 1971
Abstract In a transcription system containing λpgal8 DNA as template and Escherichia coli RNA polymerase, the addition of cyclic adenosine monophosphate receptor protein (CRP) and 3',5'-cyclic AMP causes a 15-fold increase in gal mRNA synthesis. Gal mRNA was measured by hybridizing the [3H]RNA product to the separated strands of λpgal8 DNA after ...
S. Peter Nissley   +4 more
openalex   +3 more sources

Regulation of the gal Operon of Escherichia coli by the capR Gene

open access: hybridJournal of Biological Chemistry, 1972
Abstract We have examined in greater detail the derepression of uridine diphosphate galactose-4-epimerase synthesis in capR mutants of Escherichia coli first observed by Markovitz. (Markovitz, A. (1964) Proc. Nat. Acad. Sci. U. S. A. 51, 239–246). All three enzymes of the gal operon are derepressed from 2- to 4-fold by this mutation.
George A. Mackie, David B. Wilson
openalex   +3 more sources

Cyclic AMP-dependent constitutive expression of gal operon: use of repressor titration to isolate operator mutations. [PDF]

open access: bronzeProceedings of the National Academy of Sciences, 1983
When the gal operator region is present in a multicopy plasmid it binds to all ("titrates") the gal repressor and "induces" the chromosomal gal operon. To make operator mutations (Oa) with reduced affinity toward the repressor, plasmid DNA was irradiated with UV light and mutant derivatives were isolated that were unable to release the chromosomal gal ...
Meher Irani   +4 more
openalex   +5 more sources

Beyond FimH: Diversity and Relevance of Carbohydrate-Binding Fimbrial Proteins in Escherichia coli. [PDF]

open access: yesChembiochem
This review discusses fimbrial lectins found in Escherichia coli (E. coli) other than FimH, which could be targeted to treat E. coli pathogenicity as an alternative to antibiotic treatment. Escherichia coli (E. coli) is responsible for multiple diseases in humans and animals.
Dulawa OR   +3 more
europepmc   +2 more sources

Studies with the Cyclic Adenosine Monophosphate Receptor and Stimulation of in Vitro Transcription of the Gal Operon

open access: hybridJournal of Biological Chemistry, 1974
Abstract To elucidate further how cyclic adenosine 3' : 5'-monophosphate (cyclic AMP) and the cyclic AMP receptor are able to stimulate gal mRNA synthesis we have utilized an antiserum specific for cyclic AMP receptor and also cyclic GMP as inhibitors of cyclic AMP and cyclic AMP receptor action.
Wayne B. Anderson   +2 more
openalex   +3 more sources

Transcriptional activation of the glycolytic las operon and catabolite repression of the gal operon in Lactococcus lactis are mediated by the catabolite control protein CcpA [PDF]

open access: bronzeMolecular Microbiology, 1998
The Lactococcus lactis ccpA gene, encoding the global regulatory protein CcpA, was identified and characterized. Northern blot and primer extension analyses showed that the L. lactis ccpA gene is constitutively transcribed from a promoter that does not contain a cre sequence.
Evert J. Luesink   +4 more
openalex   +5 more sources

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