In Vitro Repression of the Transcription of gal Operon by Purified gal Repressor [PDF]
Proceedings of the National Academy of Sciences, 1973 We have studied the in vitro repression of gal mRNA synthesis by the gal repressor from Escherichia coli . By use of a four-step purification procedure involving chromatography on phosphocellulose, DEAE-cellulose, and an affinity resin, the
Shigetada Nakanishi +3 more
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Transcriptional Regulation of the Streptococcus mutans gal Operon by the GalR Repressor [PDF]
Journal of Bacteriology, 1998 ABSTRACT The galactose operon of Streptococcus mutans is transcriptionally regulated by a repressor protein (GalR) encoded by the galR gene, which is divergently oriented from the structural genes of the gal operon.
Dragana Ajdić, Joseph J. Ferretti
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Journal of Biological Chemistry, 1974 Abstract To elucidate further how cyclic adenosine 3' : 5'-monophosphate (cyclic AMP) and the cyclic AMP receptor are able to stimulate gal mRNA synthesis we have utilized an antiserum specific for cyclic AMP receptor and also cyclic GMP as inhibitors of cyclic AMP and cyclic AMP receptor action.
Wayne B. Anderson +2 more
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Suppression of polarity of insertion mutations in the gal operon and N mutations in bacteriophage lambda [PDF]
Journal of Bacteriology, 1976 Bacterial mutations (psuA and psu) known for their ability to suppress the polarity on nonsense mutations are shown to suppress the polarity of certain insertion mutations in the gal operon. The short insertion, IS1 (800 nucleotide pairs), is about 15 to 50% suppressed, whereas longer insertions, IS2 (1,400 nucleotide pairs), and IS3 (1,200 nucleotide ...
Octavio Reyes López +2 more
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The use of gene fusions to study polar effects in the gal operon ofEscherichia coli [PDF]
FEMS Microbiology Letters, 1980 Maxime Schwartz, Agnès Ullmann
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Cyclic AMP-dependent constitutive expression of gal operon: use of repressor titration to isolate operator mutations. [PDF]
Proceedings of the National Academy of Sciences, 1983 When the gal operator region is present in a multicopy plasmid it binds to all ("titrates") the gal repressor and "induces" the chromosomal gal operon. To make operator mutations (Oa) with reduced affinity toward the repressor, plasmid DNA was irradiated with UV light and mutant derivatives were isolated that were unable to release the chromosomal gal ...
Meher Irani +4 more
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Escherichia coli gal operon proteins made after prophage lambda induction [PDF]
Journal of Bacteriology, 1981 Expression of the EScherichia coli gal operon under the control of the prophage lambda promoter pL leads to gross discoordinacy of gal expression. Expression of the most promoter-distal cistron galK is much greater than expression of the promoter-proximal cistron galE.
C R Merril +2 more
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The First Enzyme of the gal Operon in Inducible and Operator‐Constitutive Strains of Escherichia coli [PDF]
European Journal of Biochemistry, 1975 UDPgalactose 4‐epimerase, the product of the first structural gene of the gal operon in Escherichia coli K‐12, has been purified from strain HfrH and from its gal operator‐constitutive mutant, HfrH 81‐2. The two enzymes are purified by the same procedure, behaving identically throughout.
Roger G. Deeley +3 more
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In Vitro Transcription of the Gal Operon Requires Cyclic Adenosine Monophosphate and Cyclic Adenosine Monophosphate Receptor Protein [PDF]
Journal of Biological Chemistry, 1971 Abstract In a transcription system containing λpgal8 DNA as template and Escherichia coli RNA polymerase, the addition of cyclic adenosine monophosphate receptor protein (CRP) and 3',5'-cyclic AMP causes a 15-fold increase in gal mRNA synthesis. Gal mRNA was measured by hybridizing the [3H]RNA product to the separated strands of λpgal8 DNA after ...
S. Peter Nissley +4 more
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Structure of the galactokinase gene ofEscherichia coli, the last (?) gene of thegaloperon
Nucleic Acids Research, 1985 We present the nucleotide sequence of the galactokinase gene (galK) of Escherichia coli including its 5' and 3' flanking regions. This DNA sequence derives from the lambda gal8 transducing phage and is identical to the sequence present in the galK gene fusion vectors, pKO and pKG, commonly used to study transcriptional regulatory elements.
Christine Debouck +9 more
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