Results 31 to 40 of about 158,365 (141)

sRNA expedites polycistronic mRNA decay in Escherichia coli

Frontiers in Molecular Biosciences, 2023
In bacteria, most small RNA (sRNA) elicits RNase E-mediated target mRNA degradation by binding near the translation initiation site at the 5′ end of the target mRNA.
Heung Jin Jeon, Heung Jin Jeon, Yonho Lee, Monford Paul Abishek N, Changjo Kang, Heon M. Lim   +5 more
doaj   +1 more source

GAL Regulon in the Yeast S. cerevisiae is Highly Evolvable via Acquisition in the Coding Regions of the Regulatory Elements of the Network

Frontiers in Molecular Biosciences, 2022
GAL network in the yeast S. cerevisiae is one of the most well-characterized regulatory network. Expression of GAL genes is contingent on exposure to galactose, and an appropriate combination of the alleles of the regulatory genes GAL3, GAL1, GAL80, and ...
Rajeshkannan, Anjali Mahilkar, Supreet Saini   +2 more
doaj   +1 more source

In vivo transcription dynamics of the galactose operon: a study on the promoter transition from P1 to P2 at onset of stationary phase. [PDF]

PLoS ONE, 2011
Quantitative analyses of the 5' end of gal transcripts indicate that transcription from the galactose operon P1 promoter is higher during cell division.
Sang Chun Ji, Xun Wang, Sang Hoon Yun, Heung Jin Jeon, Hee Jung Lee, Hackjin Kim, Heon M Lim   +6 more
doaj   +1 more source

Failure of Translation Initiation of the Next Gene Decouples Transcription at Intercistronic Sites and the Resultant mRNA Generation

mBio, 2022
In Escherichia coli, transcription is coupled with translation. The polar gal operon is transcribed galE-galT-galK-galM; however, about 10% of transcription terminates at the end of galE because of Rho-dependent termination (RDT).
Heung Jin Jeon, Monford Paul Abishek N, Yonho Lee, Heon M. Lim   +3 more
doaj   +1 more source

An Engineered Reporter Phage for the Fluorometric Detection of Escherichia coli in Ground Beef

Microorganisms, 2021
Despite enhanced sanitation implementations, foodborne bacterial pathogens still remain a major threat to public health and generate high costs for the food industry.
Anqi Chen, Danhui Wang, Sam R. Nugen, Juhong Chen   +3 more
doaj   +1 more source

Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain’s functionality in vitro

Microbial Cell Factories, 2021
Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon. Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of ...
Katarina Butorac, Jasna Novak, Barbara Bellich, Lucrecia C. Terán, Martina Banić, Andreja Leboš Pavunc, Slaven Zjalić, Paola Cescutti, Jagoda Šušković, Blaženka Kos   +9 more
doaj   +1 more source

Regulation of Microbial Metabolic Rates Using CRISPR Interference With Expanded PAM Sequences

Frontiers in Microbiology, 2020
Genome-editing CRISPR/Cas9 technology has led to the development of artificial transcriptional repressors, also known as CRISPR interference (CRISPRi).
Bumjoon Kim, Hyun Ju Kim, Sang Jun Lee
doaj   +1 more source

Constitutive versus responsive gene expression strategies for growth in changing environments. [PDF]

PLoS ONE, 2011
Microbes respond to changing environments by adjusting gene expression levels to the demand for the corresponding proteins. Adjusting protein levels is slow, consequently cells may reach the optimal protein level only by a time when the demand changed ...
Nico Geisel
doaj   +1 more source

Misincorporation of Galactose by Chondroitin Synthase of Escherichia coli K4: From Traces to Synthesis of Chondbiuronan, a Novel Chondroitin-Like Polysaccharide

Biomolecules, 2020
Chondroitin synthase KfoC is a bifunctional enzyme which polymerizes the capsular chondroitin backbone of Escherichia coli K4, composed of repeated β3N-acetylgalactosamine (GalNAc)-β4-glucuronic acid (GlcA) units. Sugar donors UDP-GalNAc and UDP-GlcA are
Mélanie Leroux, Julie Michaud, Eric Bayma, Sylvie Armand, Sophie Drouillard, Bernard Priem   +5 more
doaj   +1 more source

Comparative Transcriptomic Analysis of Streptococcus thermophilus TH1436 and TH1477 Showing Different Capability in the Use of Galactose

Frontiers in Microbiology, 2018
Streptococcus thermophilus is a species widely used in the dairy industry for its capability to rapidly ferment lactose and lower the pH. The capability to use galactose produced from lactose hydrolysis is strain dependent and most of commercial S ...
Sabrina Giaretta, Laura Treu, Laura Treu, Veronica Vendramin, Vinícius da Silva Duarte, Armin Tarrah, Stefano Campanaro, Viviana Corich, Alessio Giacomini   +8 more
doaj   +1 more source