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A sensitive micro-immunoassay using β-galactosidase/ anti-β-galactosidase complexes
Journal of Immunological Methods, 1987This paper describes a new sensitive microELISA based on enzyme/anti-enzyme complexes following an unlabelled antibody bridging step. beta-Galactosidase/anti-beta-galactosidase complexes were made using a monoclonal antibody raised against bacterial (E. coli) beta-galactosidase and enzyme activity was quantified with a fluorogenic substrate. Because of
H, Durbin, W F, Bodmer
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1974
Publisher Summary The enzyme β-galactosidase is one of several oligosaccharide-splitting enzymes that have been purified and subsequently characterized to varying degrees both physically and mechanistically. It is found in a wide range of biological sources in both the plant and animal kingdoms, although the principal source of study has been in the ...
Edward Steers, Pedro Cuatrecasas
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Publisher Summary The enzyme β-galactosidase is one of several oligosaccharide-splitting enzymes that have been purified and subsequently characterized to varying degrees both physically and mechanistically. It is found in a wide range of biological sources in both the plant and animal kingdoms, although the principal source of study has been in the ...
Edward Steers, Pedro Cuatrecasas
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1974
Publisher Summary The α-galactosidase from the coffee bean is one of the few capable of hydrolyzing the nonreducing terminal α-D-galactopyranosyl residue of blood group B antigens. As part of the studies on the structure of these antigens in human erythrocytes, a method has been developed for the rapid and complete purification of the enzyme in high ...
Noam Harpaz, Harold M. Flowers
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Publisher Summary The α-galactosidase from the coffee bean is one of the few capable of hydrolyzing the nonreducing terminal α-D-galactopyranosyl residue of blood group B antigens. As part of the studies on the structure of these antigens in human erythrocytes, a method has been developed for the rapid and complete purification of the enzyme in high ...
Noam Harpaz, Harold M. Flowers
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Recent innovations in immobilization of β-galactosidases for industrial and therapeutic applications
Biotechnology Advances, 2022Jingwen Zhang, Lili Lu
exaly
1972
Publisher Summary This chapter presents information on the chemistry and enzymology of β-galactosidases. β-Galactosidases have been found in numerous microorganisms, animals, and plants. Tests for fermentation of lactose play an important role in diagnostic bacteriology of Enterobacteriaceae, so the occurrence of β-galactosidase in gram-negative rods
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Publisher Summary This chapter presents information on the chemistry and enzymology of β-galactosidases. β-Galactosidases have been found in numerous microorganisms, animals, and plants. Tests for fermentation of lactose play an important role in diagnostic bacteriology of Enterobacteriaceae, so the occurrence of β-galactosidase in gram-negative rods
openaire +1 more source

