Results 1 to 10 of about 699,193 (277)

A novel cold-active β-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization

Microbial Cell Factories, 2011
Background β-D-Galactosidases (EC 3.2.1.23) catalyze the hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides. Cold-active β-D-galactosidases have recently become a focus of attention of researchers and dairy product ...
Wierzbicka-Woś Anna, Cieśliński Hubert, Wanarska Marta, Kozłowska-Tylingo Katarzyna, Hildebrandt Piotr, Kur Józef   +5 more
doaj   +1 more source

Overviewing the Application of β-Galactosidase “Immobilized on Nanoparticles” in Dairy Industries

Brazilian Archives of Biology and Technology, 2021
Owing to the excellent catalytic potential, β-galactosidase (EC: 3.2.1.23) has been exploited as an important industrial enzyme for obtaining galactooligosaccharides (GOS) and lactose-free products in dairy industries.
Asim Muhammed Alshanberi, Majed Ahmed Al-Shaeri, Shakeel Ahmed Ansari   +2 more
doaj   +1 more source

Synthesis of β(1 → 3) and β(1 → 6) galactooligosaccharides from lactose and whey using a recombinant β-galactosidase from Pantoea anthophila

Electronic Journal of Biotechnology, 2021
Background: Milk whey, a byproduct of the dairy industry has a negative environmental impact, can be used as a raw material for added-value compounds such as galactooligosaccharides (GOS) synthesis by β-galactosidases.
Claudia V. Yañez-Ñeco, Fadia V. Cervantes, Lorena Amaya-Delgado, Antonio O. Ballesteros, Francisco J. Plou, Javier Arrizon   +5 more
doaj   +1 more source

A rapid permeabilization procedure for accurate quantitative determination of β-galactosidase activity in yeast cells [PDF]

FEMS Microbiology Letters, 1995
A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces cerevisiae, for quantitative in situ assays of beta-galactosidase activity. Yeast cells are permeabilized by incubation in buffer containing 0.2% of the detergent sodium lauroyl sarcosinate without any need for washing or ...
openaire   +2 more sources

The N-glycan glycoprotein deglycosylation complex (Gpd) from Capnocytophaga canimorsus deglycosylates human IgG. [PDF]

PLoS Pathogens, 2011
C. canimorsus 5 has the capacity to grow at the expenses of glycan moieties from host cells N-glycoproteins. Here, we show that C. canimorsus 5 also has the capacity to deglycosylate human IgG and we analyze the deglycosylation mechanism.
Francesco Renzi, Pablo Manfredi, Manuela Mally, Suzette Moes, Paul Jenö, Guy R Cornelis   +5 more
doaj   +1 more source

Aspergillus nidulans: A Potential Resource of the Production of the Native and Heterologous Enzymes for Industrial Applications

International Journal of Microbiology, 2020
Aspergillus nidulans is a filamentous fungus that is a potential resource for industrial enzymes. It is a versatile fungal cell factory that can synthesize various industrial enzymes such as cellulases, β-glucosidases, hemicellulases, laccases, lipases ...
Amit Kumar
doaj   +1 more source

Low copy number vectors for expression of fused genes to β-galactosidase inEscherichia coli [PDF]

FEMS Microbiology Letters, 1991
A series of six expression vectors, pXM184Lac.A, B, C, pXM184Z.A, B, C, based on the low copy plasmid pACYC184 that allow for expression of proteins fused to beta-galactosidase in Escherichia coli is described. A level of 50,000 units of beta-galactosidase is routinely observed and is easily identifiable on protein gels.
openaire   +2 more sources

Purification and some properties of phospho-β-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201 [PDF]

FEMS Microbiology Letters, 2002
Phospho-beta-galactosidase (P-beta-gal; EC 3.2.1.85) is induced during growth of Leptotrichia buccalis ATCC 14201 on lactose and lactulose. The enzyme has been purified to electrophoretic homogeneity (M(r) approximately 53 kDa, pI approximately 4.8), and kinetic parameters have been determined using the chromogenic analog o-nitrophenyl-beta-D ...
openaire   +2 more sources

Engineering the β-galactosidase from Aspergillus oryzae for making lactose-free and no-sugar-added yogurt

Journal of Dairy Science
: Yogurt usually contains 5% to 7% sugar and 3% to 5% lactose. As β-galactosidases can hydrolyze lactose and improve sweetness, they have the potential to produce lactose-free (LF) and no-sugar-added (NSA) yogurt.
Miao Miao, Shusen Li, Shaoqing Yang, Qiaojuan Yan, Zhixuan Xiang, Zhengqiang Jiang   +5 more
doaj   +1 more source

IMOBILIZAÇÃO DA ENZIMA Î’-GALACTOSIDASE DE Aspergillus oryzae EM RESINAS DE TROCA IÔNICA

, 2012
Este trabalho apresenta como objetivo estudar o processo de imobilização da enzima β-galactosidase de Aspergillus oryzae por adsorção, utilizando como suporte resinas de troca iônica Duolite A-568, Duolite S-761, Dowex Marathon A, Dowex Marathon C e Amberlite 252 Na.
Santana, Larissa Nayhara Soares, Guidini, Carla Zanella, Ribeiro, Eloízio Júlio   +2 more
openaire   +1 more source