Sequence‐Based and Functional Analysis for the Discovery of N‐Glycan Degrading Glycosidases From the Microbial Metagenome of the Infant Gut [PDF]
The role of bacterial glycosyl hydrolases (GHs) in degrading free human milk oligosaccharides is well documented. However, their activity on glycoconjugates is less well known.
Irene Boscá‐Sánchez +2 more
doaj +2 more sources
Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium [PDF]
Galactosides are major carbohydrates that are found in plant cell walls and various prebiotic oligosaccharides. Studying the detailed biochemical functions of β-galactosidases in degrading these carbohydrates is important.
Yutaka Nakazawa +14 more
doaj +2 more sources
In vitro investigations on the impact of fermented dairy constituents on fecal microbiota composition and fermentation activity [PDF]
Fermented dairy constitutes a major dietary source and contains lactose as the main carbohydrate and living starter cultures, which can encounter the intestinal microbiota after ingestion.
Qing Li +6 more
doaj +2 more sources
Structural Comparison of Different Galacto-oligosaccharide Mixtures Formed by β-Galactosidases from Lactic Acid Bacteria and Bifidobacteria. [PDF]
The LacLM-type β-galactosidase from Lactobacillus helveticus DSM 20075 expressed in both Escherichia coli (EcoliBL21Lhβ-gal) and Lactobacillus plantarum (Lp609Lhβ-gal) was tested for their potential to form galacto-oligosaccharides (GOS) from lactose ...
Kittibunchakul S +4 more
europepmc +3 more sources
Transferase Activity of Lactobacillal and Bifidobacterial β-Galactosidases with Various Sugars as Galactosyl Acceptors. [PDF]
The β-galactosidases from Lactobacillus reuteri L103 (Lreuβgal), Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 (Lbulβgal), and Bifidobacterium breve DSM 20281 (Bbreβgal-I and Bbreβgal-II) were investigated in detail with respect to their ...
Arreola SL +5 more
europepmc +4 more sources
Purification and characterization of a β-galactosidase fromSclerotinia sclerotiorum [PDF]
Sclerotinia sclerotiorum secretes β-galactosidase, giving very low activity when cultivated in a medium with glucose as the carbon source. This production is greatly enhanced during growth on different pectin related polysaccharides. An extracellular β-galactosidase was purified 26-fold by ammonium sulfate precipitation, gel filtration and ion exchange
Christine Riou +2 more
openaire +1 more source
The occurrence of β-galactosidase and β-phosphogalactosidase inLactobacillus caseistrains [PDF]
Several strains of Lactobacillus casei of different origins were compared and it was observed that lactose metabolism varied from one strain to the other. Certain strains contained a β-galactosidase, others a β-phosphogalactosidase and others contain both.
J. Jimeno, M. Casey, F. Hofer
openaire +1 more source
Analysis of Domain Architecture and Phylogenetics of Family 2 Glycoside Hydrolases (GH2). [PDF]
In this work we report a detailed analysis of the topology and phylogenetics of family 2 glycoside hydrolases (GH2). We distinguish five topologies or domain architectures based on the presence and distribution of protein domains defined in Pfam and ...
David Talens-Perales +4 more
doaj +1 more source
Much attention has recently been paid to β-Galactosidases (β-D-galactoside galactohidrolase; EC 3.2.1.23), commonly known as lactases, due to the lactose intolerance of the human population and the importance of dairy products in the human diet.
Katia Liburdi, Marco Esti
doaj +1 more source
Effect of tunicamycin on α-galactosidase secretion byAspergillus nidulansand the importance ofN-glycosylation [PDF]
Aspergillus nidulans released α-galactosidase into the culture medium during the exponential growth on either lactose or galactose as the only carbon source. This enzyme is a glycoprotein. Its treatment with endoglycosidases produces a reduction in its molecular mass but the resulting enzyme conserved some of their carbohydrate components in addition ...
Santiago RÃos +2 more
openaire +1 more source

