Results 31 to 40 of about 4,834 (282)

Anaerobic expression of thegyrAgene demonstrated by β-galactosidase activity ingyrA::Mu-lacfusion derivatives ofEscherichia coli [PDF]

FEMS Microbiology Letters, 1988
E. coli C600Δ lac was infected with a Mu- lac defective phage. Lysogens resistant to ampicillin (25 µg/ml) and nalidixic acid (20 µg/ml) were unable to grow anaerobically and insensitive to nalidixic acid (150 µg/ml), indicating formation of gyrA ::Mu- lac fusions.
Nobuto Yamamoto, Mary L. Droffner
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β-Galactosidase-Producing Isolates in Mucoromycota: Screening, Enzyme Production, and Applications for Functional Oligosaccharide Synthesis

Journal of Fungi, 2021
β-Galactosidases of Mucoromycota are rarely studied, although this group of filamentous fungi is an excellent source of many industrial enzymes. In this study, 99 isolates from the genera Lichtheimia, Mortierella, Mucor, Rhizomucor, Rhizopus and ...
Bettina Volford, Mónika Varga, András Szekeres, Alexandra Kotogán, Gábor Nagy, Csaba Vágvölgyi, Tamás Papp, Miklós Takó   +7 more
doaj   +1 more source

Purification and molecular properties of an α-galactosidase synthesized and secreted byAspergillus nidulans [PDF]

FEMS Microbiology Letters, 1993
alpha-Galactosidases from mycelial extract and culture filtrate of Aspergillus nidulans have been purified to homogeneity and utilised to obtain polyclonal antibodies anti-alpha-galactosidase. The enzymatic characteristics and the cross reactivity of the antibodies suggest that alpha-galactosidases isolated from the two sources were the same enzyme ...
S, Ríos, A M, Pedregosa, I, Fernández Monistrol, F, Laborda   +3 more
openaire   +2 more sources

Discovery of novel enzymes with industrial potential from a cold and alkaline environment by a combination of functional metagenomics and culturing [PDF]

, 2014
BACKGROUND: The use of cold-active enzymes has many advantages, including reduced energy consumption and easy inactivation. The ikaite columns of SW Greenland are permanently cold (4-6°C) and alkaline (above pH 10), and the microorganisms living there ...
Jan Vester, Mikkel Glaring, Peter Stougaard   +2 more
core   +2 more sources

Experimental conditions may affect reproducibility of the beta-galactosidase assay [PDF]

FEMS Microbiology Letters, 1992
Several experimental conditions and parameters contributing to the determination of beta-galactosidase activity, as proposed in Miller's assay, were studied. Use of the absorbance correction factor and the nature and concentration of permeabilizing agents were taken into account as different experimental conditions.
GIACOMINI, ALESSIO, CORICH, VIVIANA, F. J. Ollero, SQUARTINI, ANDREA, M. P. Nuti   +4 more
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Cloning of the phospho-β-galactosidase gene inEscherichia colifrom lactose-negative mutants ofStreptococcus mutansisolated following random mutagenesis with plasmid pVA891 clone banks [PDF]

FEMS Microbiology Letters, 1992
In order to mutagenize Streptococcus mutans a marker rescue plasmid, pVA891, was employed. The plasmid was ligated with Sau3AI digested chromosomal DNA fragments from S. mutans GS-5IS3 and the resultant plasmids were amplified in Escherichia coli.
Yutaka Sato, Yasuhito Yamamoto, Harutoshi Kizaki, Howard K. Kuramitsu   +3 more
openaire   +1 more source

Cloning, Expression, Purification, and Characterization of β-Galactosidase from Bifidobacterium longum and Bifidobacterium pseudocatenulatum

Molecules, 2022
Expression and purification of β-galactosidases derived from Bifidobacterium provide a new resource for efficient lactose hydrolysis and lactose intolerance alleviation. Here, we cloned and expressed two β-galactosidases derived from Bifidobacterium. The
Mingzhu Du, Shuanghong Yang, Tong Jiang, Tingting Liang, Ying Li, Shuzhen Cai, Qingping Wu, Jumei Zhang, Wei Chen, Xinqiang Xie   +9 more
doaj   +1 more source

Synthesis of Oligosaccharides Derived from Lactulose (OsLu) Using Soluble and Immobilized Aspergillus oryzae β-Galactosidase [PDF]

, 2016
β-Galactosidase from Aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (OsLu) by transgalactosylation.
Batista-Viera, F., Cardelle-Cobas, A., Corzo, N., Corzo-Martínez, M., Giacomini, C., Irazoqui, G., Olano, A.   +6 more
core   +4 more sources

Characterization and molecular cloning of a heterodimeric β-galactosidase from the probiotic strainLactobacillus acidophilusR22 [PDF]

FEMS Microbiology Letters, 2007
Beta-galactosidase from the probiotic strain Lactobacillus acidophilus R22 was purified to apparent homogeneity by ammonium sulphate fractionation, hydrophobic interaction, and affinity chromatography. The enzyme is a heterodimer consisting of two subunits of 35 and 72 kDa, as determined by gel electrophoresis.
Thu-Ha, Nguyen, Barbara, Splechtna, Stanimira, Krasteva, Wolfgang, Kneifel, Klaus D, Kulbe, Christina, Divne, Dietmar, Haltrich   +6 more
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Selection of lactic acid bacteria strain for simultaneous production of α- and β-galactosidases

Zaštita Materijala, 2016
Galactosidases are group of enzymes gaining increasing attention in recent times due to the fact they catalyse hydrolysis of galatose-containing oligosaccharide, which reduce digestibility of various food and feed products.
Milica Carević, Katarina Banjanac, Marija Ćorović, Sonja Jakovetić, Ana Milivojević, Sekulić Maja Vukašinović, Dejan Bezbradica   +6 more
doaj   +1 more source