High gene expression inEscherichia coliof recombinant alginate lyase as a fused protein with β-galactosidase α-peptide [PDF]
FEMS Microbiology Letters, 1995 Escherichia coli LE392 (pAL28) was previously isolated as a positive clone harboring the alginate lyase gene (aly) from an alginate-degrading strain, Pseudomonas sp. OS-ALG-9. The plasmid pAL205, one of the constructs obtained after successive subcloning of pAL28, gave the highest expression of aly in E. coli cells.
K, Fujiyama +3 more
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Conserved domains (E < 0.01) in β-galactosidases isolated from 12AC metagenomic library clones. [PDF]
, 2017 Conserved domains (E < 0.01) in β-galactosidases isolated from 12AC metagenomic library clones.
Trevor C. Charles (575161) +6 more
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Biochemical characterization of digestive carbohydrases in Moroccan locust, Dociostaurus maroccanus Thunberg (Orthoptera: Acrididae) [PDF]
Arthropods, 2020 The Moroccan locust, Dociostaurus maroccanus (Thunberg), is an agricultural pest which has caused considerable agricultural damage and economic importance.
Bahareh Rafiei +2 more
doaj
Lactobacillus fermentumCRL 722 is able to deliver active α-galactosidase activity in the small intestine of rats [PDF]
FEMS Microbiology Letters, 2005 alpha-galactooligosaccharides (alpha-GOS) found in legumes such as soybeans can cause gastrointestinal disorders since mammals lack alpha-galactosidase (alpha-Gal) in the small intestine which is necessary for their hydrolysis. Lactobacillus fermentum CRL 722 is a lactic acid bacterium (LAB) capable of degrading alpha-GOS due to its elevated alpha-Gal ...
Leblanc, Jean Guy +3 more
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β-Galactosidases from Jack Bean and Streptococcus Have Different Cleaving Abilities towards Fucose-Containing Sugars [PDF]
, 2011 We examined the sugar-cleaving abilities of β-galactosidases from jack bean and Streptococcus towards sugars containing fucose residues, and found that jack bean β-galactosidase has an ability to cleave the β1-3 linkage between galactose (Gal) and fucose
武内, 智春 +7 more
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A study of β-galactosidases from thermoacidophiles [PDF]
, 2013 Microorganisms that grow under multiple stress conditions, such as thermoacidophiles, are a rich source of novel glycosyl hydrolases. β-Galactosidases isolated from these extreme microorganisms would be of academic interest by extending the knowledge ...
Jayne Murphy (12602566)
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Insertion of a 27 amino acid viral peptide in different zones ofEscherichia coliβ-galactosidase: Effects on the enzyme activity [PDF]
FEMS Microbiology Letters, 1994 Seven internal, putatively exposed regions of Escherichia coli beta-galactosidase have been explored regarding their tolerance to insertions of large foreign peptides. Small sequence modifications, including amino acid substitutions and small deletions, were introduced into the lacZ gene to generate unique BamHI restriction sites. By using these mutant
A, Benito, A, Villaverde
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Sensory Assessment of Bi-Enzymatic-Treated Glucose-Galactose Syrup
Fermentation, 2023 There are a variety of ways to make glucose-galactose syrup (GGS) and other products of lactose hydrolysis; therefore, research is still ongoing and will undoubtedly result in improved methods and lower costs.
Kristine Majore, Inga Ciprovica
doaj +1 more source
Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]
FEMS Microbiology Letters, 2008 A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao +8 more
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MOESM1 of Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates [PDF]
, 2019 Additional file 1: Table S1. List of bifidobacterial genes used for comparative purposes. Figure S1. In silico analysis and functional characterization of β-galactosidases. Figure S2.
Barry Schoemaker (6246293) +7 more
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