Results 41 to 50 of about 4,834 (282)

Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice ( L.) [PDF]

, 2008
Background Many plant β-galactosidases (Bgals) have been well characterized and their deduced biological functions mainly involve degradation of structural pectins, xyloglucans or arabinogalactoproteins in plant cell walls.
Waraporn Tanthanuch, Mallika Chantarangsee, Janjira Maneesan, James R Ketudat Cairns   +3 more
core   +2 more sources

Continuous Packed Bed Reactor with Immobilized β-Galactosidase for Production of Galactooligosaccharides (GOS) [PDF]

, 2017
The β-galactosidase from Bacillus circulans was covalently attached to aldehyde-activated (glyoxal) agarose beads and assayed for the continuous production of galactooligosaccharides (GOS) in a packed-bed reactor (PBR).
Ballesteros Olmo, Antonio, Fernández Arrojo, Lucía, Plou Gasca, Francisco José, Rodríguez-Colinas, Bárbara, Santos-Moriano, Paloma   +4 more
core   +1 more source

α-Galactosidase A fromPseudomonas fluorescenssubsp.cellulosa: cloning, high level expression and its role in galactomannan hydrolysis [PDF]

FEMS Microbiology Letters, 2000
A library of Pseudomonas fluorescens subsp. cellulosa genomic DNA, constructed in lambda ZAPII, was screened for alpha-D-galactosidase activity. The DNA inserts from six galactosidase-positive clones were rescued into plasmids. Restriction digestion and Southern analysis revealed that each of the plasmids contained a common DNA sequence.
J R, Halstead, M P, Fransen, R Y, Eberhart, A J, Park, H J, Gilbert, G P, Hazlewood   +5 more
openaire   +2 more sources

Biochemical characterization of digestive carbohydrases in Moroccan locust, Dociostaurus maroccanus Thunberg (Orthoptera: Acrididae) [PDF]

Arthropods, 2020
The Moroccan locust, Dociostaurus maroccanus (Thunberg), is an agricultural pest which has caused considerable agricultural damage and economic importance.
Bahareh Rafiei, Mohammad Ghadamyari, Sohrab Imani, et al.   +2 more
doaj  

Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]

FEMS Microbiology Letters, 2008
A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao, Lili, Lu, Min, Xiao, Qinpeng, Wang, Yu, Lu, Chunhui, Liu, Peng, Wang, Hidehiko, Kumagai, Kenji, Yamamoto   +8 more
openaire   +2 more sources

A Specific Activity-Based Probe to Monitor Family GH59 Galactosylceramidase, the Enzyme Deficient in Krabbe Disease [PDF]

, 2017
Galactosylceramidase (GALC) is the lysosomal β-galactosidase responsible for the hydrolysis of galactosylceramide. Inherited deficiency in GALC causes Krabbe disease, a devastating neurological disorder characterized by accumulation of galactosylceramide
Aerts, Johannes M F G, Biela-Banas, Anna, Boot, Rolf G., Cachón‐González, M. Begoña, Cox, Timothy M., Florea, Bogdan I., Herrera Moro, Daniela, Kallemeijn, Wouter W., Kim, Nee Na, Marques, André R. A., Martin, Olivier R., Nelson, Jessica K., Ottenhoff, Roelof, Overkleeft, Herman S., Scheij, Saskia, van Roomen, Cindy P.A.A., Verhoek, Marri, Willems, Lianne I.   +17 more
core   +1 more source

High gene expression inEscherichia coliof recombinant alginate lyase as a fused protein with β-galactosidase α-peptide [PDF]

FEMS Microbiology Letters, 1995
Escherichia coli LE392 (pAL28) was previously isolated as a positive clone harboring the alginate lyase gene (aly) from an alginate-degrading strain, Pseudomonas sp. OS-ALG-9. The plasmid pAL205, one of the constructs obtained after successive subcloning of pAL28, gave the highest expression of aly in E. coli cells.
K, Fujiyama, H, Maki, S, Kinoshita, T, Yoshida   +3 more
openaire   +2 more sources

Bifidobacteria exhibit social behavior through carbohydrate resource sharing in the gut [PDF]

, 2017
Bifidobacteria are common and frequently dominant members of the gut microbiota of many animals, including mammals and insects. Carbohydrates are considered key carbon sources for the gut microbiota, imposing strong selective pressure on the complex ...
Hevia, Arancha, Margolles Barros, Abelardo, Milani, Christian, Sánchez García, Borja, Ventura, Marco   +4 more
core   +1 more source

Antigenicity of a viral peptide displayed on β-galactosidase fusion proteins is influenced by the presence of the homologous partner protein [PDF]

FEMS Microbiology Letters, 1996
Several beta-galactosidase fusion proteins have been constructed containing the entire VP1 protein from foot-and-mouth disease virus (FMDV) [Corchero et al. (1996) J. Biotechnol. in press]. The antigenicity of the major immunodominant site A (13 amino acids in length) within the VP1 protein has been studied in competitive ELISA using a panel of seven ...
J L, Corchero, A, Villaverde
openaire   +2 more sources

Les polymères de mannose en production animale. 2. Les enzymes de dégradation des mannanes dans l’alimentation des porcs et des volailles

INRAE Productions Animales, 2021
Les enzymes capables de dégrader les mannanes appartiennent à plusieurs familles d’hydrolases qui se distinguent par les sites de coupure sur ces polysaccharides.
Estelle BONNIN, Michel LESSIRE, Nathaële WACRENIER, Fabien ALLEMAN   +3 more
doaj   +1 more source