Power and limitations of electrophoretic separations in proteomics strategies [PDF]
, 2009 Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold +225 more
core +4 more sources
Revista Brasileira de Ciência do Solo, 2009 Os ácidos húmicos (AH) estimulam diretamente vários processos fisiológicos que promovem o crescimento vegetal, especialmente do sistema radicular.
Natália de Oliveira Aguiar +5 more
doaj +1 more source
Purification of Mg2+-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects [PDF]
, 2005 A new procedure for the partial purification of Mg2+-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg2+-PAP; EC 3.1.3.4) from rat liver cytosol is described, using protein precipitation with MgCl2, gel filtration on Sephacryl S ...
Butterwith +10 more
core +1 more source
Identification of novel components of Trypanosoma brucei editosomes [PDF]
, 2003 The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Carmean, Nicole +6 more
core +2 more sources
Gel chromatographic characterization of immunoreactive adrenocorticotropin in patients with ACTH hypersecretion [PDF]
, 1989 We investigated the molecular size of circulating immunoreactive ACTH by gel chromatography in patients with ACTH hypersecretion due to various disorders of the hypothalamic-pituitary-adrenal axis.
A Chatelain +19 more
core +1 more source
Discrimination of Car Headlight Plastic by Gel Permeation Chromatography
Journal of Forensic Science and Medicine, 2015 Car headlight plastic as a kind of evidence often can be seen in traffic accidents and some other cases. We tested 20 brands of car headlight plastic using gel chromatography. The data were processed using the Statistical Package for the Social Sciences (
Bo Wang, Yangke Quan, Hongling Guo
doaj +1 more source
A Monomer-to-Trimer Transition of the Human Mitochondrial Transcription Termination Factor (mTERF) Is Associated with a Loss of in Vitro Activity [PDF]
, 2004 The human mitochondrial transcription termination factor (mTERF) is a nuclear-encoded 39-kDa protein that recognizes a mtDNA segment within the mitochondrial tRNALeu(UUR) gene immediately adjacent to and downstream of the 16 S rRNA gene. Binding of mTERF
Asin-Cayuela, Jordi +2 more
core +1 more source
A gel-based method for purification of apolipoprotein A-I from small volumes of plasma
Journal of Lipid Research, 2010 We present here a gel-based method for rapid purification of apolipoprotein A-I (apoA-I) from small volumes of human plasma. After isolation of high density lipoprotein from plasma, the apoA-I protein was separated by electrophoresis and the apoA-I band ...
Rachel J. Brace +3 more
doaj +1 more source
Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii. [PDF]
, 2018 Correct folding and post-translational modifications are vital for therapeutic proteins to elicit their biological functions. Osteopontin (OPN), a bone regenerative protein present in a range of mammalian cells, is an acidic phosphoprotein with multiple ...
Guo, Shengchun +5 more
core +2 more sources
Analysis of chitosan molecular weight profile by electrophoresis in a porosity step gradient polyacrylamide gel [PDF]
The Ukrainian Biochemical Journal, 2022 Chitosan is biocompatible and biodegradable natural biopolymer widely applied in various fields of biology, medicine, and pharmacy, however, its effects significantly depend on the degree of polymerization (DP) and the degree of deacetylation (DDA) of ...
N. O. Manko +3 more
doaj +1 more source