Results 21 to 30 of about 401,087 (313)

Preparation of antibodies type IgY against Salmonella typhi lipopolysaccharide in chicken eggs

مجلة مركز بحوث التقنيات الاحيائية, 2012
ipopolysaccharide was extracted from local isolate Sallmonella typhi (previously isolated and characterized) by hot EDTA method, and the extract was partially purified by gel filtration chromatography on sepharose Cl-6B gel.
Mouruj A. Alaubydi, Susan Ahmad, Muayad Sabri   +2 more
doaj   +1 more source

Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

Molecules, 2017
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li, Qin Liu, Yajuan Cui, Dong Li, Hexiang Wang, Tzi Bun Ng   +5 more
doaj   +1 more source

Desalination effect on FⅧ components: a compartive study among 5 desalination methods

Zhongguo shuxue zazhi
Objective To compare the desalination effects of five desalination methods and their effects on the components for human coagulation factor Ⅷ(FⅧ), and provide reference for selection of protein desalination methods.
Renjun PEI, Xi DU, Pan SUN, Xiaoyue LI, Peng JIANG, Changqing LI, Fangzhao LIN, Haijun CAO   +7 more
doaj   +1 more source

Evaluation of gel chromatography for plasma lipoprotein fractionation

Journal of Lipid Research, 1970
The fractionation of lipoproteins of normal and hyperlipidemic subjects on a column of 2% agarose was compared with ultracentrifugation and paper electrophoresis procedures. The following results were obtained.
Teizo Sata, David L. Estrich, Peter D.S. Wood, Laurance W. Kinsell   +3 more
doaj   +1 more source

Purification and identification of a novel protein isolated from Panax quinquefolium and evaluation of its In vitro antioxidant properties

World Journal of Traditional Chinese Medicine, 2019
Objective: A novel protein was first purified from Panax quinquefolius L. (AGNP), and in vitro antioxidant activities of AGNP were first studied in this work.
Xue-Hui Li, Yang Yang, Zhao Yu, Ning Xu, Xiao-Lei Tang, Xue-Yuan Bai, Si-Ming Wang, Hao-Yuan Chen, Da-Qing Zhao, Li Liu, Bin Qi   +10 more
doaj   +1 more source

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source

Dry-column chromatographic isolation of fatty acid esters of phorbol from croton oil

Journal of Lipid Research, 1969
The family of mixed fatty acid esters of 4,9,12,-13,20-pentahydroxy-tiglia-1,6-dien-3-one (phorbol) has been isolated from croton oil by column chromatography on silica gel and preparative dry-column chromatography on silica gel HF/254 packed in a ...
Paul R. Ocken
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Screening, Enrichment and Structural Identification of Bitter Almond Antibacterial Peptides

Shipin gongye ke-ji
To explore the preparation, enrichment, and structural analysis of bitter almond antimicrobial peptides, in this experiment, the protein was extracted from the defatted bitter almonds, and the protein was hydrolyzed by enzymes to prepare bitter almond ...
Tao ZHANG, Jie MA, Guofang NIAN, Zhiyi ZHOU, Ting ZHANG, Jianzhong ZHOU   +5 more
doaj   +1 more source

Derivatization-free gel permeation chromatography elucidates enzymatic cellulose hydrolysis

Biotechnology for Biofuels, 2012
Background The analysis of cellulose molecular weight distributions by gel permeation chromatography (GPC) is a powerful tool to obtain detailed information on enzymatic cellulose hydrolysis, supporting the development of economically viable biorefinery ...
Engel Philip, Hein Lea, Spiess Antje C
doaj   +1 more source