Results 11 to 20 of about 1,126,750 (353)

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

open access: yesMass Spectrometry Reviews 28, 5 (2009) 816-43, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins or peptides, can rely on chromatography or electrophoresis.
Aebersold   +225 more
arxiv   +6 more sources

A new bond fluctuation method for a polymer undergoing gel electrophoresis [PDF]

open access: yes, 1998
We present a new computational methodology for the investigation of gel electrophoresis of polyelectrolytes. We have developed the method initially to incorporate sliding motion of tight parts of a polymer pulled by an electric field into the bond fluctuation method (BFM).
B. H. Zimm   +18 more
arxiv   +3 more sources

Two-dimensional gel electrophoresis in proteomics: A tutorial [PDF]

open access: yesJournal of Proteomics 74, 10 (2011) 1829-41, 2011
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the
##Righetti   +108 more
arxiv   +6 more sources

Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myeloïd cell lines nuclear proteomes [PDF]

open access: yesJournal of Proteomics (2012) epub ahead of print, 2012
One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids. Several experimental procedures have been tested to increase, in the nuclear extract, the ratio of nuclear ...
Chevallet, Mireille   +5 more
arxiv   +6 more sources

Magnetophoresis of Tagged Polymers [PDF]

open access: yes, 1996
We present quantitative results for the drift velocity of a polymer in a gel if a force (e.g. through an electric or magnetic field) acts on a tag, attached to one of its ends. This is done by introducing a modification of the Rubinstein-Duke model for electrophoresis of DNA.
Barkema, G. T., Schütz, G. M.
arxiv   +4 more sources

Transport in polymer-gel composites: Theoretical methodology and response to an electric field [PDF]

open access: yesJ Fluid Mech. (2006) vol 551 pp 405--433, 2005
A theoretical model of electromigrative, diffusive and convectivetransport polymer-gel composites is presented. Bulk properties are derived from the standard electrokinetic model with an impenetrable charged sphere embedded in an electrolyte-saturated Brinkman medium. Because the microstructure can be carefully controlled, these materials are promising
Hill, Reghan J.
arxiv   +3 more sources

Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]

open access: yes, 1982
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő   +3 more
core   +1 more source

Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]

open access: yes, 2008
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core   +2 more sources

An Analysis System for DNA Gel Electrophoresis Images Based on Automatic Thresholding an Enhancement [PDF]

open access: yesIEEE Electro/Information Technology, 2007, 2016
Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues.
arxiv   +1 more source

Gel versus capillary electrophoresis genotyping for categorizing treatment outcomes in two anti-malarial trials in Uganda

open access: yesMalaria Journal, 2010
Background Molecular genotyping is performed in anti-malarial trials to determine whether recurrent parasitaemia after therapy represents a recrudescence (treatment failure) or new infection.
Hubbard Alan E   +4 more
doaj   +1 more source

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