Power and limitations of electrophoretic separations in proteomics strategies [PDF]
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins or peptides, can rely on chromatography or electrophoresis.
Aebersold+225 more
arxiv +6 more sources
A new bond fluctuation method for a polymer undergoing gel electrophoresis [PDF]
We present a new computational methodology for the investigation of gel electrophoresis of polyelectrolytes. We have developed the method initially to incorporate sliding motion of tight parts of a polymer pulled by an electric field into the bond fluctuation method (BFM).
B. H. Zimm+18 more
arxiv +3 more sources
Two-dimensional gel electrophoresis in proteomics: A tutorial [PDF]
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the
##Righetti+108 more
arxiv +6 more sources
Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myeloïd cell lines nuclear proteomes [PDF]
One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids. Several experimental procedures have been tested to increase, in the nuclear extract, the ratio of nuclear ...
Chevallet, Mireille+5 more
arxiv +6 more sources
Magnetophoresis of Tagged Polymers [PDF]
We present quantitative results for the drift velocity of a polymer in a gel if a force (e.g. through an electric or magnetic field) acts on a tag, attached to one of its ends. This is done by introducing a modification of the Rubinstein-Duke model for electrophoresis of DNA.
Barkema, G. T., Schütz, G. M.
arxiv +4 more sources
Transport in polymer-gel composites: Theoretical methodology and response to an electric field [PDF]
A theoretical model of electromigrative, diffusive and convectivetransport polymer-gel composites is presented. Bulk properties are derived from the standard electrokinetic model with an impenetrable charged sphere embedded in an electrolyte-saturated Brinkman medium. Because the microstructure can be carefully controlled, these materials are promising
Hill, Reghan J.
arxiv +3 more sources
Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő+3 more
core +1 more source
Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core +2 more sources
An Analysis System for DNA Gel Electrophoresis Images Based on Automatic Thresholding an Enhancement [PDF]
Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues.
arxiv +1 more source
Background Molecular genotyping is performed in anti-malarial trials to determine whether recurrent parasitaemia after therapy represents a recrudescence (treatment failure) or new infection.
Hubbard Alan E+4 more
doaj +1 more source