Results 31 to 40 of about 1,156,214 (332)
Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]
, 2006 Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C +3 more
core +2 more sources
Polysaccharide as a Separation Medium for Gel Electrophoresis
PolysaccharidesGel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa +5 more
doaj +1 more source
Denaturing Polyacrylamide Gel Electrophoresis [PDF]
Current Protocols in Nucleic Acid Chemistry, 1994 AbstractThin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single‐stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all ...
Albright, Lisa M., Slatko, Barton E.
openaire +4 more sources
A rapid high-resolution method for resolving DNA topoisomers
BMC Research Notes, 2018 Objective Agarose gel electrophoresis has been the mainstay technique for the analysis of DNA samples of moderate size. In addition to separating linear DNA molecules, it can also resolve different topological forms of plasmid DNAs, an application useful
Lesley A. Mitchenall +4 more
doaj +1 more source
Comparative 2-DE protein analysis in a 3-D geometry gel
BioTechniques, 2007 Two-dimensional gel electrophoresis (2-DE) separation has not been considered suitable for large-scale comparative protein expression studies due to its limited throughput.
Robert Ventzki +4 more
doaj +1 more source
Manual for starch gel electrophoresis: A method for the detection of genetic variation [PDF]
, 1987 The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2) preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts ...
Aebersold, Paul B. +4 more
core
Genetic Differentiation Among Three Species of \u3ci\u3eParadosa\u3c/i\u3e (Arachnida: Lycosidae) [PDF]
, 2017 Allozymic variation in nine protein producing loci was examined in three species of Pardosa using starch gel electrophoresis. Allozyme frequencies showed a high degree of geographic uniformity among conspecific populations. Estimated heterozygosities for
Elliott, N. C +3 more
core +2 more sources
FEBS Letters, EarlyView.Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield +3 more
wiley +1 more source
Investigation of Gel Electrophoresis Stains
Al-SalamJournal for Medical ScienceGel electrophoresis is essential in molecular biology and biochemistry for separating and analyzing DNA, RNA, and proteins. UV sensitivity and compatibility have led to the use of ethidium bromide stains.
ibrahim ramzi hadi +4 more
doaj +1 more source
FEBS Letters, EarlyView.Phytohormone brassinosteroid‐induced gene regulation by the transcription factor BIL1/BZR1 involves redox‐dependent DNA‐binding alternation and interaction with the transcription factor PIF4. The reduced BIL1/BZR1 dimer binds preferred cis‐elements, while oxidation alters its oligomerization state and disrupts DNA‐binding ability.
Shohei Nosaki +4 more
wiley +1 more source