Results 31 to 40 of about 1,126,750 (353)
Two-dimensional gel electrophoresis in proteomics: past, present and future [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist.
arxiv +1 more source
Variations on a theme: Changes to electrophoretic separations that can make a difference [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
arxiv +1 more source
Semi-denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE)
Bio-Protocol, 2014 Pathological proteins in neurodegenerative diseases suffer a conformational change to a misfolded amyloid state. Such pathological event leads to the aggregation of these proteins that indefinitely propagates as an altered form of itself, and harbor ...
Laura Molina-García +1 more
doaj +1 more source
A rapid high-resolution method for resolving DNA topoisomers
BMC Research Notes, 2018 Objective Agarose gel electrophoresis has been the mainstay technique for the analysis of DNA samples of moderate size. In addition to separating linear DNA molecules, it can also resolve different topological forms of plasmid DNAs, an application useful
Lesley A. Mitchenall +4 more
doaj +1 more source
Stochastic modeling for the COMET-assay [PDF]
, 2003 We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data ...
Boulesteix, Anne-Laure +2 more
core +3 more sources
Journal of Pharmacopuncture, 2006 Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi +2 more
doaj +1 more source
The Whereabouts of 2D Gels in Quantitative Proteomics [PDF]
Methods in Molecular Biology -Clifton then Totowa- 893 (2012)
25-35, 2012 Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series.
arxiv +1 more source
Comparative Proteomics and Difference Gel Electrophoresis
BioTechniques, 2007 The goal of comparative proteomics is to analyze proteome changes in response to development, disease, or environment. This is a two-step process in which proteins within cellular extracts are first fractionated to reduce sample complexity, and then the ...
Jonathan Minden
doaj +1 more source
, 2022 Gel electrophoresis is used to separate and analyze biomolecules, such as DNA, RNA, and proteins. By applying an electric field to a gel matrix, charged molecules migrate at varying speeds based on their size and charge. This process enables researchers to visualize and quantify nucleic acids and proteins, making it an essential tool for genetic ...
openaire +2 more sources
Polysaccharide as a Separation Medium for Gel Electrophoresis
PolysaccharidesGel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa +5 more
doaj +1 more source