Results 241 to 250 of about 3,576,469 (305)

A new multiphasic buffer system for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of proteins and peptides with molecular masses 100 000–1000, and their detection with picomolar sensitivity

Electrophoresis, 1991
A novel multiphasic buffer system for high resolution sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of dansylated and nondansylated proteins/peptides in the relative molecular mass (Mr) range of 100 000–1000 is described. The system, based on
J. Wiltfang, N. Arold, V. Neuhoff
semanticscholar   +1 more source

Protein extraction and two-dimensional gel electrophoresis of proteins in the marine mussel Mytilus galloprovincialis: an important tool for protein expression studies, food quality and safety assessment.

The Journal of the Science of Food and Agriculture, 2013
BACKGROUND Shellfish farming is an important economic activity that provides society with a valuable source of food. Analyses of the protein content and metabolism of shellfish are therefore of utmost importance to monitor the presence and effects of ...
A. Campos, M. Puerto, A. Prieto, A. Cameán, A. Almeida, A. Coelho, V. Vasconcelos   +6 more
semanticscholar   +1 more source

Starch-Gel Electrophoresis of Wheat Proteins

Nature, 1960
IT has been claimed that starch-gel electrophoresis may have a higher resolving power than that of the Tiselius apparatus for serum proteins1. In spite of its potential value, there have been no reports in the literature of the application of this technique to the study of wheat proteins.
J. A. D. Ewart, G. A. H. Elton
openaire   +3 more sources

The identification of Enterobacteriaceae from breweries: combined use and comparison of API 20E system, gel electrophoresis of proteins and gas chromatography of volatile metabolites.

Journal of Applied Bacteriology, 1981
The API 20E system was applied and evaluated for the identification of 60 bacterial contaminants, isolated from South African lager beer breweries and tentatively identified as Enterobacteriaceae.
H. V. van Vuuren, K. Kersters, J. De Ley, D. Toerien   +3 more
semanticscholar   +1 more source

Two-dimensional polyacrylamide gel electrophoresis of proteins labeled with the fluorophore monobromobimane prior to first-dimensional isoelectric focusing: imaging of the fluorescent protein spot patterns using a cooled charge-coupled device.

Analytical Biochemistry, 1993
A new method for visualizing 2D protein spot patterns is described whereby proteins containing sulfhydryl groups are labeled with the fluorophore monobromobimane prior to the isoelectric focusing step of 2D polyacrylamide gel electrophoresis.
V. Urwin, P. Jackson
semanticscholar   +1 more source

The separation of membrane proteins by polyacrylamide gel electrophoresis

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1971
A modified Takayama (K. Takayama, D. H. Mac Lennanan, A. Tzagoloff and C. D. Stoner, Arch. Biochem. Biophys., 114 (1966) 223.) method for acrylamide gel electrophoresis of membrane proteins is described. The method uses acetic acid, urea, phenol and mercaptoethanol as the solvent for solubilizing the membranes.
Tushar K. Ray, G.V. Marinetti
openaire   +3 more sources

The interaction of proteins during gel electrophoresis

Journal of the Science of Food and Agriculture, 1966
AbstractReversible polymerisation and A + B ⇌ C type reactions among proteins during zone electrophoresis may give rise to tailing, extra bands and mobility changes. One or more of these signs are likely to appear with the milder type of interaction, i.e. equilibrium constant in the range 102–108, whether the time to equilibrium is measured in hours or
openaire   +3 more sources

Resolving Proteins for Immunoblotting by Gel Electrophoresis

Cold Spring Harbor Protocols, 2018
This protocol describes Tris/glycine SDS–polyacrylamide gel electrophoresis, also known as SDS discontinuous gel electrophoresis or the Laemmli electrophoresis system. The gel-casting unit is assembled and tested to make sure that there are no leaks. Ammonium persulfate and tetramethylethylenediamine are added to the separating monomer solution, and ...
openaire   +3 more sources

A gel eluter for recovery of proteins separated by polyacrylamide gel electrophoresis

Analytical Biochemistry, 1982
Abstract An apparatus for the electrophoretic elution of proteins from polyacrylamide gel slices is described. The eluter is inexpensive, easy to build, and can be constructed to fit many available slab-gel apparatuses. A high yield of protein, concentrated in a small volume of solution, can be electrophoretically separated and recovered within a 24 ...
George L. Gerton, Nathan J. Wardrip, Jerry L. Hedrick   +2 more
openaire   +3 more sources

PREPARATIVE ELECTROPHORESIS OF PROTEINS IN ACRYLAMIDE GELS*

Annals of the New York Academy of Sciences, 1964
SUMMARY(1) A simple apparatus is described for adaption of the “disc” electrophoresis techniques to a preparative procedure that permits analytic and radioactive monitoring of the effluent fractions automatically.(2) The efficiency of the procedure for separating a model system consisting of ribonuclease, trypsin, and chymotrypsin has been demonstrated.
openaire   +3 more sources