The guideline for western blotting assay
Food Frontiers, 2022 Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang +5 more
doaj +1 more source
BioTechniques, 2014 Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl +6 more
doaj +1 more source
Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients [PDF]
Electrophoresis 18 (31/03/1997) 307-16, 2006 Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high.
arxiv +1 more source
Protein electrophoretic migration data from custom and commercial gradient gels
Data in Brief, 2016 This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller +2 more
doaj +1 more source
Western blotting of native proteins from agarose gels
BioTechniques, 2022 We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma +10 more
doaj +1 more source
Chemical differences of atherosclerotic plaques in native and bypass human coronary arteries and diseased and non-diseased human aortas [PDF]
, 2007 Cardiovascular disease, primarily atherosclerosis involves a number of distinct processes that are associated with plaque development. The use of differential gel electrophoresis to determine differences between proteins produced in native and bypass ...
Washington, Samuel Jean
core +2 more sources
Isolation and Analytical Characterization of Local Malaysian Leech Saliva
International Islamic University Malaysia Engineering Journal, 2011 Leech saliva contains biologically active compounds that are mainly proteins and peptides. In this study a modified and smooth extraction method of saliva was used without leeches' scarification.
Mohamed Alaama +4 more
doaj +1 more source
An Analysis System for DNA Gel Electrophoresis Images Based on Automatic Thresholding an Enhancement [PDF]
IEEE Electro/Information Technology, 2007, 2016 Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues.
arxiv +1 more source
Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis
Proteomes, 2016 The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy +2 more
doaj +1 more source
Obtaining Of β-Lactoglobulin By Gel Filtration Of Cow Milk Whey [PDF]
, 2019 Milk whey proteins carry out a number of important biological functions and also they are precursors of many biologically active peptides (antihypertensive peptides, antagonists of opioid receptors, regulators of intestinal motility, immunomodulatory ...
Datsyshyn, K. (Kateryna) +2 more
core +3 more sources