Results 21 to 30 of about 3,553,338 (335)
Isolation and Analytical Characterization of Local Malaysian Leech Saliva
International Islamic University Malaysia Engineering Journal, 2011 Leech saliva contains biologically active compounds that are mainly proteins and peptides. In this study a modified and smooth extraction method of saliva was used without leeches' scarification.
Mohamed Alaama +4 more
doaj +1 more source
Western blotting of native proteins from agarose gels
BioTechniques, 2022 We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma +10 more
doaj +1 more source
An Analysis System for DNA Gel Electrophoresis Images Based on Automatic Thresholding an Enhancement [PDF]
IEEE Electro/Information Technology, 2007, 2016 Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues.
arxiv +1 more source
Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis
Proteomes, 2016 The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy +2 more
doaj +1 more source
Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins
BioTechniques, 1998 Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj +1 more source
Mapping cells and sub-cellular organelles on 2-D gels: ‘new tricks for an old horse’ [PDF]
, 1995 Nowadays, investigators in all fields are faced with the identification of unknown, up- or down-regulated, modified proteins that they are trying to identify.
Dahlback, B. +5 more
core +4 more sources
Antibodies, 2022 In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the ...
Teruo Akuta +11 more
doaj +1 more source
Variations on a theme: Changes to electrophoretic separations that can make a difference [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
arxiv +1 more source
Biology, 2013 In this article, we illustrate the application of difference in-gel electrophoresis for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used as a tissue model of dystrophinopathy.
Steven Carberry +3 more
doaj +1 more source
Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae. [PDF]
, 1988 The application of two-dimensional electrophoretic procedures to structural and genetic studies of seed proteins from Poaceae (including the cultivated cereals) and Fagaceae is described.
Aragoncillo Ballesteros, Cipriano +5 more
core +2 more sources