Results 21 to 30 of about 916,712 (391)

Obtaining Of β-Lactoglobulin By Gel Filtration Of Cow Milk Whey [PDF]

, 2019
Milk whey proteins carry out a number of important biological functions and also they are precursors of many biologically active peptides (antihypertensive peptides, antagonists of opioid receptors, regulators of intestinal motility, immunomodulatory ...
Datsyshyn, K. (Kateryna), Storozh, L. (Liudmyla), Yukalo, V. (Volodymyr)   +2 more
core   +3 more sources

Protein electrophoretic migration data from custom and commercial gradient gels

Data in Brief, 2016
This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller, Brandon Roman, Eric M. Norstrom   +2 more
doaj   +1 more source

Study of protein expresion [sic] in peri-infarct tissue after cerebral ischemia [PDF]

, 2015
In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal ...
Agulla, Jesús, Blanco, Miguel, Brea, David, Campos, Francisco, Castillo, José, Dávalos, Antoni, Gevaert, Kris, Ramos-Cabrer, Pedro, Sobrino, Tomás, Staes, An   +9 more
core   +5 more sources

Isolation and Analytical Characterization of Local Malaysian Leech Saliva

International Islamic University Malaysia Engineering Journal, 2011
Leech saliva contains biologically active compounds that are mainly proteins and peptides. In this study a modified and smooth extraction method of saliva was used without leeches' scarification.
Mohamed Alaama, Manar AlNajjar, Abdualrahman Abdualkader, Abbas Mohammad, and Ahmed Merzouk   +4 more
doaj   +1 more source

Western blotting of native proteins from agarose gels

BioTechniques, 2022
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta   +10 more
doaj   +1 more source

Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis

Proteomes, 2016
The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy, Paul Dowling, Kay Ohlendieck   +2 more
doaj   +1 more source

Mapping cells and sub-cellular organelles on 2-D gels: ‘new tricks for an old horse’ [PDF]

, 1995
Nowadays, investigators in all fields are faced with the identification of unknown, up- or down-regulated, modified proteins that they are trying to identify.
Dahlbäck, Björn,, de Vries, R., Dullaart, R. P. F., Groen, A. K., Lund University., Nielsen, L. B., Plomgaard, P.   +6 more
core   +4 more sources

Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins

BioTechniques, 1998
Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj   +1 more source

A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

Antibodies, 2022
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the ...
Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa   +11 more
doaj   +1 more source

False-Negative Urine Protein Electrophoresis by Semiautomated Gel Electrophoresis [PDF]

Clinical Chemistry, 2004
Urine is evaluated by electrophoretic methods both to gather information about the location and degree of damage within the nephron and to detect and quantify monoclonal free light chains (Bence Jones proteins). For many years, urine protein electrophoresis consisted of concentrating the urine (usually >50-fold) followed by manual application of the ...
Stephen J. Ebrom, David F. Keren, Ronald Gulbranson   +2 more
openaire   +3 more sources