Results 21 to 30 of about 864,514 (341)

Mapping cells and sub-cellular organelles on 2-D gels: ‘new tricks for an old horse’ [PDF]

, 1995
Nowadays, investigators in all fields are faced with the identification of unknown, up- or down-regulated, modified proteins that they are trying to identify.
Dahlbäck, Björn,, de Vries, R., Dullaart, R. P. F., Groen, A. K., Lund University., Nielsen, L. B., Plomgaard, P.   +6 more
core   +4 more sources

Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins

BioTechniques, 1998
Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj   +1 more source

Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]

, 2006
Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C, Cornelis, Rita, Moens, Luc, Vanhaecke, Frank   +3 more
core   +2 more sources

A Comparison of gel (Hydragel 30) and capillary (Capillarys III Tera) electrophoresis for the characterization of human serum proteins

Practical Laboratory Medicine, 2021
Objectives: To compare gel (Hydrasys 2 from Sebia) and capillary (Capillarys III Tera, Sebia) electrophoresis for the characterization of human serum proteins.
Brittney M. Howard, Annie Kuh, LuAnn Rezavi, Patrizio Caturegli   +3 more
doaj   +1 more source

A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

Antibodies, 2022
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the ...
Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa   +11 more
doaj   +1 more source

Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling. [PDF]

PLoS ONE, 2013
Complexome profiling is a novel technique which uses shotgun proteomics to establish protein migration profiles from fractionated blue native electrophoresis gels.
Hans J C T Wessels, Rutger O Vogel, Robert N Lightowlers, Johannes N Spelbrink, Richard J Rodenburg, Lambert P van den Heuvel, Alain J van Gool, Jolein Gloerich, Jan A M Smeitink, Leo G Nijtmans   +9 more
doaj   +1 more source

Single-cell western blotting. [PDF]

, 2014
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h.
Herr, Amy E, Hughes, Alex J, Kang, Chi-Chih, Schaffer, David V, Spelke, Dawn P, Xu, Zhuchen   +5 more
core   +2 more sources

Application of Fluorescence Two-Dimensional Difference In-Gel Electrophoresis as a Proteomic Biomarker Discovery Tool in Muscular Dystrophy Research

Biology, 2013
In this article, we illustrate the application of difference in-gel electrophoresis for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used as a tissue model of dystrophinopathy.
Steven Carberry, Margit Zweyer, Dieter Swandulla, Kay Ohlendieck   +3 more
doaj   +1 more source

Agarose gel serum protein electrophoresis in cats with and without lymphoma and preliminary results of tandem mass fingerprinting analysis [PDF]

, 2011
<b>Background</b>: Serum electrophoretic profiles in cats are poorly characterized with respect to the protein components of the globulin fractions, and interpretation of the electrophoretograms has routinely been done in ignorance of the ...
Baker, Bence, Bing, Ceron, Cerón, Choi-Miura, Correa, Corzo, Dust, Eckersall, Furukawa, Giordano, González-Ramón, Hahn, Hofmann-Lehmann, Jeppsson, Keay, Kristensen, Lampreave, Laurell, MacEwen, McGrotty, Merlo, Moore, Nielsen, Ogilvie, Paltrinieri, Paltrinieri, Prenni, Roman, Saguchi, Selting, Sparkes, Stockham, Vail, Vavricka, Wiechselbaum   +36 more
core   +1 more source

Western Blot Analysis of Chloroplast HSP70B in Chlorella Species

Bio-Protocol, 2013
Western blotting allows for the specific detection of proteins by an antibody of interest. This protocol utilizes isolation of total proteins protocol for Chlorella vulgaris prior to gel electrophoresis. After electrophoresis, the selected antibodies are
Stephka Chankova, Zhana Mitrovska, Nadezhda Yurina   +2 more
doaj   +1 more source