Results 51 to 60 of about 3,475,365 (278)
Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]
Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C+3 more
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Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools.
Yunita Armiyanti+6 more
doaj +1 more source
Silver Staining of 2D Electrophoresis Gels [PDF]
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and ...
arxiv +1 more source
Sodium Dodecyl Sulphate Polyacrylamide gel electrophoresis (SDS-PAGE) and Silver Staining of distinct samples [PDF]
SDS-PAGE is a technique to separate proteins from biological samples according to molecular mass. There are highly virulent and invasive pathogens that rapidly invade the host tissue barriers and causes infection in human body.
Kaur, Raminderjeet
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The repton model of gel electrophoresis [PDF]
We discuss the repton model of agarose gel electrophoresis of DNA. We review previous results, both analytic and numerical, as well as presenting a new numerical algorithm for the efficient simulation of the model, and suggesting a new approach to the model's analytic solution.
arxiv +1 more source
Western Analysis of Histone Modifications (Aspergillus nidulans)
Western blotting allows for the specific detection of proteins and/or modifications of proteins by an antibody of interest. This protocol utilizes a crude nuclei extraction protocol for Aspergillus nidulans to enrich for histones and other nuclear ...
Alexandra Soukup, Nancy Keller
doaj +1 more source
pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by
Marina Hanneken+2 more
doaj +1 more source
Evaluation of Urine Proteins by Capillary Electrophoresis
Capillary electrophoresis (CE) is a laboratory method usually used to separate proteins in body fluids such as serum, cerebrospinal fluid, or urine. Separation of proteins in urine can have clinical applications for evaluating samples from healthy dogs ...
Paula Navarro+2 more
doaj +1 more source
Electrophoretic separation of proteins [PDF]
There are several structural and storage proteins in the tissues of Crustacea. Some of them are metallo-proteins, some are enzymic in nature. Several electrophoretic procedures were used for the separation of these proteins.
Ravindranath, M H, Subhashini, M H
core
Comparison of protein extraction methods suitable for proteomics analysis in seedling roots of Jerusalem artichoke under salt (NaCl) stress [PDF]
An efficient protein extraction method is a prerequisite for successful implementation of proteomics. In this study, seedling roots of Jerusalem artichoke were treated with the concentration of 250 mM NaCl for 36 h.
Chen, Q, Shen, SH, Zhang, MD
core +2 more sources