Results 61 to 70 of about 3,475,365 (278)

Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis

open access: yesJournal of Pharmacopuncture, 2006
Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi   +2 more
doaj   +1 more source

Analytical purification of a 60-kDa target protein of artemisinin detected in Trypanosoma brucei brucei

open access: yesData in Brief, 2015
Here we describe the isolation and purity determination of Trypanosoma brucei (T. b.) brucei candidate target proteins of artemisinin. The candidate target proteins were detected and purified from their biological source (T. b.
Benetode Konziase
doaj   +1 more source

WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins. [PDF]

open access: yesPLoS ONE, 2017
Post-translational modification with O-linked β-N-acetylglucosamine (O-GlcNAc) occurs selectively on serine and/or threonine residues of cytoplasmic and nuclear proteins, and dynamically regulates their molecular functions.
Yuji Kubota   +2 more
doaj   +1 more source

Comparison of extraction methods and nutritional benefits of proteins of milk and dairy products: A review

open access: yesCzech Journal of Food Sciences, 2022
Proteins are the second most essential macromolecules after nucleic acids. This article aimed at the comparative analysis of extraction methods and nutritional benefits of milk and dairy products.
Hira Javed   +9 more
doaj   +1 more source

Analysis of soybean-proteins (Glycine max) by two-dimensional electrophoresis and MALDI-TOF [PDF]

open access: yes, 2006
Food allergy is a growing problem in the world today, which makes it important to find out which proteins in food that causes allergic reactions. Soybeans are one of the most common food allergens in many countries and little is known about it allergenic
Olken, Lena
core  

Single-cell western blotting. [PDF]

open access: yes, 2014
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h.
Herr, Amy E   +5 more
core   +2 more sources

How to use 2D gel electrophoresis in plant proteomics [PDF]

open access: yesMethods in Molecular Biology -Clifton then Totowa- 1072 (2014) 43-50, 2013
Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable nowadays.Nevertheless, 2D gel-based proteomics still has valuable features, and sometimes unique ones, which make it often an ...
arxiv   +1 more source

Chromogranins, widespread in endocrine and nervous tissue, bind Ca2+ [PDF]

open access: yes, 1985
The proteinaceous components of the secretory vesicle contents isolated from bovine adrenal medulla bind Ca2+ (number of binding sites, 152 ± 52 nmol Ca2+ per mg protein; dissociation constant, 54 ± 8 μM (n = 5)).
Gratzl, Manfred, Reiffen, F. U.
core   +1 more source

Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis [PDF]

open access: yesMethods in molecular biology (Clifton, N.J.) 528 (2009) 259-67, 2009
Because of the outstanding ability of two-dimensional electrophoresis to separate complex mixtures of intact proteins, it would be advantageous to apply it to all types of proteins, including hydrophobic and membrane proteins. Unfortunately, poor solubility hampers the analysis of these molecules.
arxiv   +1 more source

The effect of high temperature on chemical structure of red blood corpuscles membranes for employees in ovens of Ramadi glass factory – Iraq. [PDF]

open access: yesمجلة جامعة الانبار للعلوم الصرفة, 2012
This study include investigation the effects of high temperature on the chemical structure of RBCs membranes in thirty workers of the state coperation of glass industries in Ramadi - Iraq , thirty technicians and administrators for interior control and ...
Safa Kamil Al-Amin   +3 more
doaj   +1 more source

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