Results 151 to 160 of about 95,204 (199)

Cloning cassettes containing the reporter gene xylE

Gene, 1994
Two pUC-derived vectors containing the promoterless xylE gene (encoding catechol 2,3-dioxygenase) of Pseudomonas putida mt-2 were constructed. The t(o) transcriptional terminator of phage lambda was placed downstream from the stop codon of xylE. The new vectors, pXT1 and pXT2, contain xylE and the t(o) terminator within a cloning cassette which can be ...
Clark E., CIRVILLERI, Gabriella
openaire   +3 more sources

Mobile Gene Cassettes and Integrons in Evolution

Annals of the New York Academy of Sciences, 1999
ABSTRACT: Integrons and the site‐specific recombination systems encoded by them provide a simple mechanism for the addition of new genes to bacterial chromosomes. Although there is substantial divergence among the four known integron‐encoded integrases, they all recognize the recombination sites, known as 59‐base elements, that are associated with ...
R M, Hall, C M, Collis, M J, Kim, S R, Partridge, G D, Recchia, H W, Stokes   +5 more
openaire   +2 more sources

Evolution of ATP-binding cassette transporter genes

Current Opinion in Genetics & Development, 1995
The transport of molecules across lipid membranes is an essential function of all living organisms. One of the families of genes that have evolved to carry out this function is that which encodes the ATP-binding cassette proteins. These molecules use active transport to pump specific molecules across membranes, and the genes that encode them are found ...
M, Dean, R, Allikmets
openaire   +2 more sources

Mobile Gene Cassettes and Integrons

Molecular Biology, 2002
Integrons are unique natural systems for capturing and spreading the antibiotic resistance genes among Gram-negative bacteria. Gene transfer into small genomes and into plasmids is via site-specific recombination. Integrons act as receptors of antibiotic resistance cassettes.
openaire   +1 more source

Site‐specific insertion of gene cassettes into integrons

Molecular Microbiology, 1993
SummarySite‐specific insertion of gene cassettes into the insert region of integrons has been demonstrated. Insertion was only observed if the integron DNA integrase was expressed in the recipient cell and if the cassette DNA was ligated prior to transformation.
C M, Collis, G, Grammaticopoulos, J, Briton, H W, Stokes, R M, Hall   +4 more
openaire   +2 more sources

Class 1 Integrons, Gene Cassettes, Mobility, and Epidemiology

European Journal of Clinical Microbiology & Infectious Diseases, 1999
Integrons are genetic elements that, although unable to move themselves, contain gene cassettes that can be mobilized to other integrons or to secondary sites in the bacterial genome. The majority of approximately 60 known gene cassettes encode resistance to antibiotics. Recently, a number of gene cassettes encoding extended-spectrum beta-lactamases or
A C, Fluit, F J, Schmitz
openaire   +2 more sources

Heterologous URA3MX cassettes for gene replacement inSaccharomyces cerevisiae

Yeast, 1999
Heterologous gene replacement cassettes are powerful tools for dissecting gene function in Saccharomyces cerevisiae. Their primary advantages over homologous gene replacement cassettes include reduced gene conversion (leading to efficient site-specific integration of the cassette) and greater independence of strain background.
A L, Goldstein, X, Pan, J H, McCusker
openaire   +2 more sources

Origins of the mobile gene cassettes found in integrons

Trends in Microbiology, 1997
Many of the acquired antibiotic resistance genes found in enterobacteria and pseudomonads are part of small mobile elements known as gene cassettes, and other genes are also likely to be found in cassettes. The origins of the genes and the recombination sites that make up cassettes are not known, but recent analyses of available data suggest that ...
G D, Recchia, R M, Hall
openaire   +2 more sources