Results 261 to 270 of about 95,867 (295)

Heterologous URA3MX cassettes for gene replacement inSaccharomyces cerevisiae

Yeast, 1999
Heterologous gene replacement cassettes are powerful tools for dissecting gene function in Saccharomyces cerevisiae. Their primary advantages over homologous gene replacement cassettes include reduced gene conversion (leading to efficient site-specific integration of the cassette) and greater independence of strain background.
A L, Goldstein, X, Pan, J H, McCusker
openaire   +2 more sources

Plant Trait Gene Expression Cassette Design

2015
Plant biotechnology depends on robust control of trait gene expression to produce an adequate level of protein or RNA in the appropriate cells at the correct time. Early biotechnology products depended on high protein production in most plant cells, and relied on relatively few well-characterized “promoters.” This demand continues and has expanded into
Michael Nuccio, Xi Chen, Jared Conville, Ailing Zhou, Xiaomei Liu   +4 more
openaire   +1 more source

MX Cassettes for Knocking Out Genes in Yeast

Cold Spring Harbor Protocols, 2017
Precise modifications of the Saccharomyces cerevisiae genome use marker cassettes, most often in the form of “knockout” (KO) marker cassettes, to delete genes. Many different KO marker cassettes exist, some of which require strains with specific genotypes, such as auxotrophic mutations, and others that have no strain genotype requirements, such as ...
openaire   +2 more sources

Ecology of the integron gene cassette metagenome

2022
Integrons are genetic elements that promote rapid adaptation in bacteria by capturing exogenous mobile gene cassettes. Recently, a sub-set of gene cassettes have facilitated the global spread of antibiotic resistance, however, outside of clinical settings, very little is known about the function and ecology of these cassettes.
openaire   +1 more source

Design of Muscle Gene Therapy Expression Cassette

2019
The first gene therapy drug approved by the European Regulatory Commission involves the transfer of a therapeutic gene to the muscle by adeno-associated viral vector (AAV). Now, muscle gene transfer is quickly becoming a therapy of choice for muscle and non-muscle diseases.
Yi Lai, Dongsheng Duan
openaire   +1 more source

β-Globin expression cassette 2.0: Optimizing gene therapy cassettes using RMCE

Blood Cells, Molecules, and Diseases, 2007
Romain Desprat, Eric E. Bouhassira
openaire   +1 more source

Nanopore sequencing analysis of integron gene cassettes in sewages and soils

Science of the Total Environment, 2022
Yong-Guan Zhu, Jian-Qiang Su, Hu Li
exaly  

Construction disruption cassettes for gene silencing

Clinical Biochemistry, 2011
Fateme Nafian Dehkordi, Somaye Mazaheri
openaire   +1 more source

[Mobile gene cassettes and DNA integration elements ].

Molekuliarnaia biologiia, 2002
Integrons are unique natural systems for capturing and spreading the antibiotic resistance genes among Gram-negative bacteria. Gene transfer into small genomes and into plasmids is via site-specific recombination. Integrons act as receptors of antibiotic resistance cassettes. There are known more than 50 cassettes conferring resistance to beta-lactams,
openaire   +1 more source

Random Chromosomal Gene Disruption Using Cassette Mutagenesis

2002
French A, Lewis, Brian A, Dougherty
openaire   +2 more sources