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Brief guide to gene cloning [PDF]
Molecules and Cells: Analysis and manipulation of DNA is fundamental to understand gene function and expression. Gene cloning is a routine and versatile technique for molecular biology, allowing isolation, amplification, and production of recombinant DNA molecules.
Woojin Hong +3 more
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An optimized disease resistance gene cloning workflow for wheat [PDF]
Nature CommunicationsThe cloning of disease resistance genes in wheat has been disproportionately slow, tedious and costly because of the large and complex genome. Wheat gene cloning projects in the late 1990s and early 2000s were multi-year endeavors, often spanning a ...
Yajun Wang +9 more
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Establishment of PCR Identification Method for Pig Blood Type
Shiyan dongwu yu bijiao yixue, 2023 ObjectiveXenotransplantation is an effective way to address the shortage of human organ donors, but it faces serious immune rejection reactions, including hyperacute rejection caused by blood type differences.
WANG Jiaoxiang +8 more
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Frontiers in Genetics, 2021 Cryopreservation of porcine cloned zygotes has important implications for biotechnology and biomedicine research; however, lower embryo developmental potential remains an urgent problem to be resolved.
Decai Xiang +7 more
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High-throughput FastCloning technology: A low-cost method for parallel cloning
PLoS ONE, 2022 FastCloning, a reliable cloning technique for plasmid construction, is a widely used protocol in biomedical research laboratories. Only two-step molecular manipulations are required to add a gene (cDNA) of interest into the desired vector.
Hua Jiang +9 more
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Frontiers in Genetics, 2020 Jellyfish, such as Chrysaora quinquecirrha, hold an important evolutionary position and have great ecological value. However, limited genomic resources are currently available for studying their basic genetic and development processes.
Wangxiao Xia +7 more
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IRDL cloning: a one-tube, zero-background, easy-to-use, directional cloning method improves throughput in recombinant DNA preparation. [PDF]
PLoS ONE, 2014 Rapid and efficient construction of expression vectors and subsequent transformation are basic recombinant methods for the investigation of gene functionality.
Jiancai Wang, Ronghua Xu, Aizhong Liu
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Ribosomal binding site switching: an effective strategy for high-throughput cloning constructions. [PDF]
PLoS ONE, 2012 Direct cloning of PCR fragments by TA cloning or blunt end ligation are two simple methods which would greatly benefit high-throughput (HTP) cloning constructions if the efficiency can be improved.
Yangbo Hu +6 more
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Cloning and Expression of Synthetic Plantaricin F Gene
Journal of Pure and Applied Microbiology, 2023 Antimicrobial activity of certain species of Lactobacillus bacteria were known to produce a certain type of bacteriocin called Plantaricin (Pln). In this study, the PlnF (plnF) gene (666 bp) encoding plantaricin was synthetically constructed based on a ...
Khadeeja S. Madhi +2 more
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A series of TA-based and zero-background vectors for plant functional genomics. [PDF]
PLoS ONE, 2013 With the sequencing of genomes from many organisms now complete and the development of high-throughput sequencing, life science research has entered the functional post-genome era.
Chuntao Wang +9 more
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