Results 11 to 20 of about 480,078 (261)

A series of TA-based and zero-background vectors for plant functional genomics. [PDF]

PLoS ONE, 2013
With the sequencing of genomes from many organisms now complete and the development of high-throughput sequencing, life science research has entered the functional post-genome era.
Chuntao Wang, Xianlun Yin, Xiangxiang Kong, Wansha Li, Lan Ma, Xudong Sun, Yanlong Guan, Christopher D Todd, Yongping Yang, Xiangyang Hu   +9 more
doaj   +1 more source

Endogenous leptin promotes autophagy in EBSS-induced PFCs

Animal Cells and Systems, 2019
Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated.
Deling Jiao, Zhen Yang, Lulu Wang, Binyue Hu, Jing Wang, Anyong Xu, Wenmin Cheng, Baoyu Jia, Yubo Qing, Hong-Ye Zhao, Hong-Jiang Wei   +10 more
doaj   +1 more source

Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system

Stem Cell Research & Therapy, 2019
Background Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models.
Junjun Xu, Leqian Yu, Jianxiong Guo, Jinzhu Xiang, Zheng Zheng, Dengfeng Gao, Bingbo Shi, Haiyang Hao, Deling Jiao, Liang Zhong, Yu Wang, Jun Wu, Hongjiang Wei, Jianyong Han   +13 more
doaj   +1 more source

A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system

Virology Journal, 2020
Background Reverse genetics is used in many laboratories around the world and enables the creation of tailor-made influenza viruses with a desired genotype or phenotype.
Sushant Bhat, Dagmara Bialy, Joshua E. Sealy, Jean-Remy Sadeyen, Pengxiang Chang, Munir Iqbal   +5 more
doaj   +1 more source

Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

AMB Express, 2017
The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation ...
Liting Cao, Yancheng Zhou, Lin Huang, Shiqi Dong, Yue Ma   +4 more
doaj   +1 more source

Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Indonesian Journal of Biotechnology, 2021
Streptomyces is one of mercury‐resistant bacteria which can convert Hg2+ into nontoxic Hg0 . This study aimed to identify mercury‐resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small‐scale gold mining (ASGM) area and ...
Wahyu Aristyaning Putri, Hanum Mukti Rahayu, Anis Uswatun Khasanah, Langkah Sembiring, Masashi Kawaichi, Yekti Asih Purwestri   +5 more
doaj   +1 more source

Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

Frontiers in Bioengineering and Biotechnology, 2020
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan, Yanjing Zeng, Wentao Shen, Decai Tuo, Xiaoying Li, Peng Zhou   +5 more
doaj   +1 more source

A qPCR Method to Distinguish between Expression of Transgenic and Endogenous Copies of Genes

Bio-Protocol, 2023
Study of gene function in eukaryotes frequently requires data on the impact of the gene when it is expressed as a transgene, such as in ectopic or overexpression studies. Currently, the use of transgenic constructs designed to achieve these aims is often
William Bezodis, Helen Prescott, Daniela Vlad, Hugh Dickinson   +3 more
doaj   +1 more source

A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps

Plant Methods, 2009
Background The progress and completion of various plant genome sequencing projects has paved the way for diverse functional genomic studies that involve cloning, modification and subsequent expression of target genes. This requires flexible and efficient
Etchells J Peter, Atanassov Ilian I, Atanassov Ivan I, Turner Simon R   +3 more
doaj   +1 more source

Overlap extension cloning of PCR products into a Gateway-compatible plasmid vector

BioTechniques, 2023
A PCR cloning method that combines a dual selection pGATE-1 plasmid vector and an improved overlap extension cloning was developed. This efficient and cost-effective method allows for the introduction of DNA fragments into the Gateway cloning pipeline ...
Omid Zare-Mehrjerdi, Gracie Trader, Viktor Kirik   +2 more
doaj   +1 more source