Results 11 to 20 of about 3,039,728 (253)

Towards an understanding of British public attitudes concerning human cloning. [PDF]

, 2007
The ability of scientists to apply cloning technology to humans has provoked public discussion and media coverage. The present paper reports on a series of studies examining public attitudes to human cloning in the UK, bringing together a range of ...
Coyle, Adrian, Senior, V, Walton, Chris, Senior, Victoria, Shepherd, Richard, Walton, C, Barnett, J, Moran-Ellis, J, Barnett, Julie, Shepherd, R, Coyle, A, Cooper, H, Cooper, Helen, Moran-Ellis, Jo   +13 more
core   +5 more sources

Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning [PDF]

, 2010
Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a ...
Neil G. Stoker, Stoker, N G, Francois-Xavier Chauviac, Balhana, R J C, Sikder, M H, Stoker, Neil G., Chauviac, Francois-Xavier, Sharon L. Kendall, Sikder, Mahmudul Hasan, Kendall, S L, Birkbeck College, Chauviac, F-X, Mahmudul Hasan Sikder, Kendall, Sharon L., Ricardo Balhana, Balhana, Ricardo   +15 more
core   +1 more source

Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis [PDF]

, 1986
Friehs K, Schörgendorfer K, Schwab H, Lafferty RM. Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis. Journal of Biotechnology.
Lafferty, R. M., Friehs, Karl, Schörgendorfer, K., Schwab, H.   +3 more
core   +1 more source

Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli [PDF]

, 1989
Friehs K, Bailey JE. Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli. Journal of Biotechnology. 1989;9(4):305-316.The plasmid pJMC40 containing the lon gene was transformed into E.
Bailey, J. E., Friehs, Karl
core   +1 more source

Cloning and expression of the levanase gene in Alcaligenes eutrophus H16 enables the strain to hydrolyze sucrose [PDF]

, 1989
Friehs K, Lafferty RM. Cloning and expression of the levanase gene in Alcaligenes eutrophus H16 enables the strain to hydrolyze sucrose. Journal of Biotechnology.
Lafferty, R. M., Friehs, Karl
core   +1 more source

Endogenous leptin promotes autophagy in EBSS-induced PFCs

Animal Cells and Systems, 2019
Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated.
Deling Jiao, Zhen Yang, Lulu Wang, Binyue Hu, Jing Wang, Anyong Xu, Wenmin Cheng, Baoyu Jia, Yubo Qing, Hong-Ye Zhao, Hong-Jiang Wei   +10 more
doaj   +1 more source

Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system

Stem Cell Research & Therapy, 2019
Background Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models.
Junjun Xu, Leqian Yu, Jianxiong Guo, Jinzhu Xiang, Zheng Zheng, Dengfeng Gao, Bingbo Shi, Haiyang Hao, Deling Jiao, Liang Zhong, Yu Wang, Jun Wu, Hongjiang Wei, Jianyong Han   +13 more
doaj   +1 more source

A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system

Virology Journal, 2020
Background Reverse genetics is used in many laboratories around the world and enables the creation of tailor-made influenza viruses with a desired genotype or phenotype.
Sushant Bhat, Dagmara Bialy, Joshua E. Sealy, Jean-Remy Sadeyen, Pengxiang Chang, Munir Iqbal   +5 more
doaj   +1 more source

Cloning, sequencing, and overexpression of gene 16 of salmonella bacteriophage P22 [PDF]

, 1992
Umlauf B, Dreiseikelmann B. Cloning, sequencing, and overexpression of gene 16 of salmonella bacteriophage P22. Virology. 1992;188(2):495-501.It has been suggested that gene product 16 of bacteriophage P22 forms a pore for DNA transfer and/or that it ...
Dreiseikelmann, Brigitte, Umlauf, Bettina   +1 more
core   +1 more source

Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

AMB Express, 2017
The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation ...
Liting Cao, Yancheng Zhou, Lin Huang, Shiqi Dong, Yue Ma   +4 more
doaj   +1 more source