Results 21 to 30 of about 3,039,728 (253)

Novel broad-host-range vehicles for cloning and shuffling of gene cassettes [PDF]

, 2012
Novel vectors for cloning and shuffling of gene cassettes based on minireplicon of broadhost- range RA3 plasmid from IncU incompatibility group were constructed.
Jolanta Szarlak, Bartosik, Aneta A, Markowska, Aleksandra, Anna Kulińska, Szarlak, Jolanta, Grażyna Jagura-Burdzy, Jagura-Burdzy, Grazyna, Aneta A. Bartosik, Aleksandra Markowska, Kulinska, Anna   +9 more
core   +1 more source

Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Indonesian Journal of Biotechnology, 2021
Streptomyces is one of mercury‐resistant bacteria which can convert Hg2+ into nontoxic Hg0 . This study aimed to identify mercury‐resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small‐scale gold mining (ASGM) area and ...
Wahyu Aristyaning Putri, Hanum Mukti Rahayu, Anis Uswatun Khasanah, Langkah Sembiring, Masashi Kawaichi, Yekti Asih Purwestri   +5 more
doaj   +1 more source

Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

Frontiers in Bioengineering and Biotechnology, 2020
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan, Yanjing Zeng, Wentao Shen, Decai Tuo, Xiaoying Li, Peng Zhou   +5 more
doaj   +1 more source

A qPCR Method to Distinguish between Expression of Transgenic and Endogenous Copies of Genes

Bio-Protocol, 2023
Study of gene function in eukaryotes frequently requires data on the impact of the gene when it is expressed as a transgene, such as in ectopic or overexpression studies. Currently, the use of transgenic constructs designed to achieve these aims is often
William Bezodis, Helen Prescott, Daniela Vlad, Hugh Dickinson   +3 more
doaj   +1 more source

A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps

Plant Methods, 2009
Background The progress and completion of various plant genome sequencing projects has paved the way for diverse functional genomic studies that involve cloning, modification and subsequent expression of target genes. This requires flexible and efficient
Etchells J Peter, Atanassov Ilian I, Atanassov Ivan I, Turner Simon R   +3 more
doaj   +1 more source

Overlap extension cloning of PCR products into a Gateway-compatible plasmid vector

BioTechniques, 2023
A PCR cloning method that combines a dual selection pGATE-1 plasmid vector and an improved overlap extension cloning was developed. This efficient and cost-effective method allows for the introduction of DNA fragments into the Gateway cloning pipeline ...
Omid Zare-Mehrjerdi, Gracie Trader, Viktor Kirik   +2 more
doaj   +1 more source

Cloning of a Carcinoembryonic Antigen Gene Family Member Expressed in Leukocytes of Chronic Myeloid Leukemia Patients and Bone Marrow [PDF]

, 1990
The carcinoembryonic antigen (CEA) gene family belongs to the immunoglobulin superfamily and can be subdivided into the CEA and pregnancy-specific glycoprotein subgroups.
Kolbinger, Frank, Zimmermann, Wolfgang, Grunert, Fritz, Buchegger, Franz, Berling, Barbara, Brombacher, Frank, Thompson, John A., Kleist, Sabine von   +7 more
core   +1 more source

Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination

Nature Communications, 2021
Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate
Behnam Enghiad, Chunshuai Huang, Fang Guo, Guangde Jiang, Bin Wang, S. Kasra Tabatabaei, Teresa A. Martin, Huimin Zhao   +7 more
doaj   +1 more source

Impact of air pollution and heavy metal exposure on sperm quality: A clinical prospective research study

Toxicology Reports
Exposure to air pollution poses significant risks to human health, including detrimental effects on the reproductive system, affecting both men and women.
Abilash D, Sridharan T B
doaj   +1 more source

Integrative analysis of swine infectious diseases in the context of xenotransplantation

International Journal of Infectious Diseases
Background: The initiation of clinical pig xenotransplantation trials offers the prospect of alleviating global organ shortage. However, current knowledge of swine-derived microorganisms relevant to xenotransplantation remains fragmented due to the ...
Jiayu Li, Kai Liu, Muhammad Ameen Jamal, Kaixiang Xu, Wenmin Cheng, Baoyu Jia, Hong-Ye Zhao, Hongzhou Lu, Jay A. Fishman, Hong-Jiang Wei   +9 more
doaj   +1 more source