Results 21 to 30 of about 497,526 (307)

Ribosomal binding site switching: an effective strategy for high-throughput cloning constructions. [PDF]

PLoS ONE, 2012
Direct cloning of PCR fragments by TA cloning or blunt end ligation are two simple methods which would greatly benefit high-throughput (HTP) cloning constructions if the efficiency can be improved.
Yangbo Hu, Lipeng Feng, Yunlong Li, Yong Zhang, Pei Lu, Simon Rayner, Shiyun Chen   +6 more
doaj   +1 more source

A series of TA-based and zero-background vectors for plant functional genomics. [PDF]

PLoS ONE, 2013
With the sequencing of genomes from many organisms now complete and the development of high-throughput sequencing, life science research has entered the functional post-genome era.
Chuntao Wang, Xianlun Yin, Xiangxiang Kong, Wansha Li, Lan Ma, Xudong Sun, Yanlong Guan, Christopher D Todd, Yongping Yang, Xiangyang Hu   +9 more
doaj   +1 more source

Endogenous leptin promotes autophagy in EBSS-induced PFCs

Animal Cells and Systems, 2019
Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated.
Deling Jiao, Zhen Yang, Lulu Wang, Binyue Hu, Jing Wang, Anyong Xu, Wenmin Cheng, Baoyu Jia, Yubo Qing, Hong-Ye Zhao, Hong-Jiang Wei   +10 more
doaj   +1 more source

Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system

Stem Cell Research & Therapy, 2019
Background Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models.
Junjun Xu, Leqian Yu, Jianxiong Guo, Jinzhu Xiang, Zheng Zheng, Dengfeng Gao, Bingbo Shi, Haiyang Hao, Deling Jiao, Liang Zhong, Yu Wang, Jun Wu, Hongjiang Wei, Jianyong Han   +13 more
doaj   +1 more source

A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system

Virology Journal, 2020
Background Reverse genetics is used in many laboratories around the world and enables the creation of tailor-made influenza viruses with a desired genotype or phenotype.
Sushant Bhat, Dagmara Bialy, Joshua E. Sealy, Jean-Remy Sadeyen, Pengxiang Chang, Munir Iqbal   +5 more
doaj   +1 more source

Novel broad-host-range vehicles for cloning and shuffling of gene cassettes [PDF]

, 2012
Novel vectors for cloning and shuffling of gene cassettes based on minireplicon of broadhost- range RA3 plasmid from IncU incompatibility group were constructed.
Jolanta Szarlak, Bartosik, Aneta A, Markowska, Aleksandra, Anna Kulińska, Szarlak, Jolanta, Grażyna Jagura-Burdzy, Jagura-Burdzy, Grazyna, Aneta A. Bartosik, Aleksandra Markowska, Kulinska, Anna   +9 more
core   +1 more source

Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

AMB Express, 2017
The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation ...
Liting Cao, Yancheng Zhou, Lin Huang, Shiqi Dong, Yue Ma   +4 more
doaj   +1 more source

Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Indonesian Journal of Biotechnology, 2021
Streptomyces is one of mercury‐resistant bacteria which can convert Hg2+ into nontoxic Hg0 . This study aimed to identify mercury‐resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small‐scale gold mining (ASGM) area and ...
Wahyu Aristyaning Putri, Hanum Mukti Rahayu, Anis Uswatun Khasanah, Langkah Sembiring, Masashi Kawaichi, Yekti Asih Purwestri   +5 more
doaj   +1 more source

Towards an understanding of British public attitudes concerning human cloning. [PDF]

, 2007
The ability of scientists to apply cloning technology to humans has provoked public discussion and media coverage. The present paper reports on a series of studies examining public attitudes to human cloning in the UK, bringing together a range of ...
Coyle, Adrian, Senior, V, Walton, Chris, Senior, Victoria, Shepherd, Richard, Walton, C, Barnett, J, Moran-Ellis, J, Barnett, Julie, Shepherd, R, Coyle, A, Cooper, H, Cooper, Helen, Moran-Ellis, Jo   +13 more
core   +1 more source

Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

Frontiers in Bioengineering and Biotechnology, 2020
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan, Yanjing Zeng, Wentao Shen, Decai Tuo, Xiaoying Li, Peng Zhou   +5 more
doaj   +1 more source