Results 31 to 40 of about 3,039,728 (253)

Alcohol and male infertility: is there a safe dose? Evidence of accessory gland dysfunction, oxidative stress, and sperm DNA damage

Frontiers in Reproductive Health
IntroductionAlcohol consumption is a recognized modifiable lifestyle factor affecting male reproductive health. Yet, its dose-dependent effects on accessory gland function and sperm genomic integrity remains poorly elucidated.
P. Jagadesh, T. B. Sridharan
doaj   +1 more source

pXST, a novel vector for TA cloning and blunt-end cloning

BMC Biotechnology, 2018
Background With the rapid development of sequencing technologies, increasing amount of genomic information has been accumulated. To clone genes for further functional studies in large scale, a cheap, fast and efficient cloning vector is desired.
Qin Liu, Hui-Jie Dang, Yuan-Hang Wu, Min Li, Yin-Hua Chen, Xiao-Lei Niu, Kai-Mian Li, Li-Juan Luo   +7 more
doaj   +1 more source

The Cloning of the Human Tumor Supressor Gene INGI: DNA Cloning into Plasmid Vector and DNA Analysis by Restriction Enzymes

Journal of Dentistry Indonesia, 2015
DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari, Mamoru Ouchida, Mehmet Gunduz   +2 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

ABC cloning: An efficient, simple, and rapid restriction/ligase-free method

MethodsX, 2019
DNA cloning remains the primary step before the further investigation of gene function. Restriction enzyme-based cloning methods are still widely used and numerous restriction-free cloning techniques are available as alternatives.
Samir El Qaidi, Philip R. Hardwidge
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Cloning of the Complete Gene for Carcinoembryonic Antigen [PDF]

, 1990
Carcinoembryonic antigen (CEA) is a widely used tumor marker, especially in the surveillance of colonic cancer patients. Although CEA is also present in some normal tissues, it is apparently expressed at higher levels in tumorous tissues than in ...
Schrewe, Heinrich, Bona, Marion, Zimmermann, Wolfgang, Shively, John E., Hassauer, Martin, Hefta, Laura J. F., Maruya, Aiko, Thompson, John A., Kleist, Sabine von   +8 more
core   +1 more source

Trace element dynamics and male fertility: A narrative review on lead, iron, and zinc

Journal of Trace Elements and Minerals
Background: Male infertility is an increasing global health concern, with growing evidence highlighting the role of essential trace elements and toxic metals in male reproductive physiology.
Parameswari R, Madhan Kumar P, Kasthuri K, Ganesamoorthy R, Babujanarthanam R, Sridharan TB, Abilash D, Thirugnanasambandham K   +7 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source