Results 31 to 40 of about 497,526 (307)

A qPCR Method to Distinguish between Expression of Transgenic and Endogenous Copies of Genes

Bio-Protocol, 2023
Study of gene function in eukaryotes frequently requires data on the impact of the gene when it is expressed as a transgene, such as in ectopic or overexpression studies. Currently, the use of transgenic constructs designed to achieve these aims is often
William Bezodis, Helen Prescott, Daniela Vlad, Hugh Dickinson   +3 more
doaj   +1 more source

A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps

Plant Methods, 2009
Background The progress and completion of various plant genome sequencing projects has paved the way for diverse functional genomic studies that involve cloning, modification and subsequent expression of target genes. This requires flexible and efficient
Etchells J Peter, Atanassov Ilian I, Atanassov Ivan I, Turner Simon R   +3 more
doaj   +1 more source

Overlap extension cloning of PCR products into a Gateway-compatible plasmid vector

BioTechniques, 2023
A PCR cloning method that combines a dual selection pGATE-1 plasmid vector and an improved overlap extension cloning was developed. This efficient and cost-effective method allows for the introduction of DNA fragments into the Gateway cloning pipeline ...
Omid Zare-Mehrjerdi, Gracie Trader, Viktor Kirik   +2 more
doaj   +1 more source

Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination

Nature Communications, 2021
Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate
Behnam Enghiad, Chunshuai Huang, Fang Guo, Guangde Jiang, Bin Wang, S. Kasra Tabatabaei, Teresa A. Martin, Huimin Zhao   +7 more
doaj   +1 more source

Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning

, 2010
Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a ...
Neil G. Stoker, Stoker, N G, Francois-Xavier Chauviac, Balhana, R J C, Sikder, M H, Stoker, Neil G., Chauviac, Francois-Xavier, Sharon L. Kendall, Sikder, Mahmudul Hasan, Kendall, S L, Birkbeck College, Chauviac, F-X, Mahmudul Hasan Sikder, Kendall, Sharon L., Ricardo Balhana, Balhana, Ricardo   +15 more
core   +1 more source

Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis

, 1986
Friehs K, Schörgendorfer K, Schwab H, Lafferty RM. Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis. Journal of Biotechnology.
Lafferty, R. M., Friehs, Karl, Schörgendorfer, K., Schwab, H.   +3 more
core   +2 more sources

Genomic organization, expression analysis, and chromosomal localization of the mouse PEX3 gene encoding a peroxisomal assembly protein [PDF]

, 2000
The peroxin Pex3p has been identified as an integral peroxisomal membrane protein in yeast where pex3 mutants lack peroxisomal remnant structures.
Muntau, Anja C., Muntau, AC, Mayerhofer, Peter U., Albet, Sabrina, Bugaut, Maurice, Bugaut, M, Mayerhofer, PU, Roscher, AA, Albet, S, Kammerer, S, Schmid, Thomas E., Roscher, Adelbert A., Kammerer, Stefan, Schmid, TE   +13 more
core   +1 more source

Impact of air pollution and heavy metal exposure on sperm quality: A clinical prospective research study

Toxicology Reports
Exposure to air pollution poses significant risks to human health, including detrimental effects on the reproductive system, affecting both men and women.
Abilash D, Sridharan T B
doaj   +1 more source

pXST, a novel vector for TA cloning and blunt-end cloning

BMC Biotechnology, 2018
Background With the rapid development of sequencing technologies, increasing amount of genomic information has been accumulated. To clone genes for further functional studies in large scale, a cheap, fast and efficient cloning vector is desired.
Qin Liu, Hui-Jie Dang, Yuan-Hang Wu, Min Li, Yin-Hua Chen, Xiao-Lei Niu, Kai-Mian Li, Li-Juan Luo   +7 more
doaj   +1 more source

Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli

, 1989
Friehs K, Bailey JE. Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli. Journal of Biotechnology. 1989;9(4):305-316.The plasmid pJMC40 containing the lon gene was transformed into E.
Bailey, J. E., Friehs, Karl
core   +2 more sources