Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Dead-End (dnd) Gene Cloning and Gonad-Specific Expression Pattern in Starry Flounder (Platichthys stellatus). [PDF]
Animals (Basel), 2021 Yoon JH +4 more
europepmc +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, 2016 BACKGROUND AND OBJECTIVE: Gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid used in the treatment of hypertension, diabetes, inflammation, and depression.
B Arabpour, A Esmaeili, M Rabbani
doaj
NosZ gene cloning, reduction performance and structure of Pseudomonas citronellolis WXP-4 nitrous oxide reductase. [PDF]
RSC Adv, 2022 Hu L +7 more
europepmc +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
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Faster Bacterial Gene Cloning Using the Brick into the Gateway (BiG) Protocol.
Bio Protoc, 2022 Pierdoná FG +4 more
europepmc +1 more source
Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276
FEBS Open Bio, EarlyView.Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig +7 more
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Genome-wide identification, gene cloning, subcellular location and expression analysis of SPL gene family in P. granatum L. [PDF]
BMC Plant Biol, 2021 Li B +6 more
europepmc +1 more source
ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells
FEBS Open Bio, EarlyView.Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori +8 more
wiley +1 more source