Results 251 to 260 of about 835,345 (308)
Some of the next articles are maybe not open access.
2011
Aggrecan is a large proteoglycan that plays roles in numerous tissues during vertebrate development and adult life. The 6,327-nt chick aggrecan coding sequence had been determined from overlapping clones, but a full-length cDNA, needed for use in transgenic expression studies, had not been constructed. The strategy employed to do so was to generate two
Mauricio, Cortes +3 more
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Aggrecan is a large proteoglycan that plays roles in numerous tissues during vertebrate development and adult life. The 6,327-nt chick aggrecan coding sequence had been determined from overlapping clones, but a full-length cDNA, needed for use in transgenic expression studies, had not been constructed. The strategy employed to do so was to generate two
Mauricio, Cortes +3 more
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2020
From 1999-2003, Oxford GlycoSciences (OGS) ran a successful drug discovery oncology programme to discover small molecule inhibitors of the Heparanase I enzyme (HPSE1). HPSE1 at the time was widely regarded as being the sole mammalian enzyme capable of cleaving Heparan Sulfate (HS).
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From 1999-2003, Oxford GlycoSciences (OGS) ran a successful drug discovery oncology programme to discover small molecule inhibitors of the Heparanase I enzyme (HPSE1). HPSE1 at the time was widely regarded as being the sole mammalian enzyme capable of cleaving Heparan Sulfate (HS).
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2023
The discovery of two naturally occurring biological molecules, plasmid DNA and restriction enzymes, with remarkable properties have made possible the development of methods to isolate and manipulate specific DNA fragments. Through this technology, a DNA fragment, even an entire gene and its controlling elements, can be isolated and rejoined with a ...
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The discovery of two naturally occurring biological molecules, plasmid DNA and restriction enzymes, with remarkable properties have made possible the development of methods to isolate and manipulate specific DNA fragments. Through this technology, a DNA fragment, even an entire gene and its controlling elements, can be isolated and rejoined with a ...
openaire +1 more source
2021
The discovery of two naturally occurring biological molecules, plasmid DNA and restriction enzymes, with remarkable properties have made possible the development of methods to isolate and manipulate specific DNA fragments. Through this technology, a DNA fragment, even an entire gene and its controlling elements, can be isolated and rejoined with a ...
openaire +1 more source
The discovery of two naturally occurring biological molecules, plasmid DNA and restriction enzymes, with remarkable properties have made possible the development of methods to isolate and manipulate specific DNA fragments. Through this technology, a DNA fragment, even an entire gene and its controlling elements, can be isolated and rejoined with a ...
openaire +1 more source
Journal of Natural Resources and Life Sciences Education, 2003
The nature of plant genetic engineering requires the production of many copies of the specific gene that is to be inserted into the plant cell. This lesson teaches the utilization of recombinant DNA techniques to isolate and copy a specific gene of interest.
Hain, Patricia, Lee, Don
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The nature of plant genetic engineering requires the production of many copies of the specific gene that is to be inserted into the plant cell. This lesson teaches the utilization of recombinant DNA techniques to isolate and copy a specific gene of interest.
Hain, Patricia, Lee, Don
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Molecular and General Genetics MGG, 1979
In Escherichia coli, the structural gene for purine nucleoside phosphorylase, deoD, is subject to insertional inactivation by prophage lambda. From one such secondary site lambda lysogen, strain SP265, one may isolate deletions that remove all or part of the trpR gene and other genes in the deo-thr sector of the E. coli chromosome.
W, Roeder, R L, Somerville
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In Escherichia coli, the structural gene for purine nucleoside phosphorylase, deoD, is subject to insertional inactivation by prophage lambda. From one such secondary site lambda lysogen, strain SP265, one may isolate deletions that remove all or part of the trpR gene and other genes in the deo-thr sector of the E. coli chromosome.
W, Roeder, R L, Somerville
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Urological Research, 1999
Cancer, including genitourinary malignancy, is a consequence of accumulated genetic aberrations in genes involved in crucial regulatory pathways. The result is a deregulation of cellular behaviour, leading to neoplastic transformation, uncontrolled cell proliferation and acquisition of metastatic ability. The development and perfection of techniques in
D H, Schamhart, A C, Westerhof
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Cancer, including genitourinary malignancy, is a consequence of accumulated genetic aberrations in genes involved in crucial regulatory pathways. The result is a deregulation of cellular behaviour, leading to neoplastic transformation, uncontrolled cell proliferation and acquisition of metastatic ability. The development and perfection of techniques in
D H, Schamhart, A C, Westerhof
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1985
Little is known about the molecular basis for differential gene expression during muscle development, the assembly of contractile proteins into filament lattices, or the functions of contractile protein isoforms in different muscle tissues. The small soil nematode Caenorhabditis elegans is an attractive organism in which to apply biochemical and ...
J, Karn, N J, Dibb, D M, Miller
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Little is known about the molecular basis for differential gene expression during muscle development, the assembly of contractile proteins into filament lattices, or the functions of contractile protein isoforms in different muscle tissues. The small soil nematode Caenorhabditis elegans is an attractive organism in which to apply biochemical and ...
J, Karn, N J, Dibb, D M, Miller
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Biochemical Genetics, 1994
We report the isolation of nine rat cognates of mouse homeoboxes within the four Hox gene clusters and a rat homologue of mouse IPF1 homeobox, RHbox#13A. The sequences of nine cloned homeoboxes are highly similar to those of the mouse and human homeoboxes in the Hox clusters. The restriction enzyme sites and map distances between each of the homeoboxes
Y, Sakoyama +3 more
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We report the isolation of nine rat cognates of mouse homeoboxes within the four Hox gene clusters and a rat homologue of mouse IPF1 homeobox, RHbox#13A. The sequences of nine cloned homeoboxes are highly similar to those of the mouse and human homeoboxes in the Hox clusters. The restriction enzyme sites and map distances between each of the homeoboxes
Y, Sakoyama +3 more
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2015
For gene cloning of known allergens, primers are designed according to individual allergen genes and selective amplification by PCR, however, for cloning of an unknown or unstudied species, the design of the degenerate primers is crucial to its successful amplification by PCR.
Xue-Ting Liu, Ailin Tao
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For gene cloning of known allergens, primers are designed according to individual allergen genes and selective amplification by PCR, however, for cloning of an unknown or unstudied species, the design of the degenerate primers is crucial to its successful amplification by PCR.
Xue-Ting Liu, Ailin Tao
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