Results 91 to 100 of about 2,666,439 (212)

Probing gene function in Candida albicans wild-type strains by Cas9-facilitated one-step integration of two dominant selection markers: a systematic analysis of recombination events at the target locus

mSphere
The adaptation of gene deletion methods based on the CRISPR-Cas9 system has facilitated the genetic manipulation of the pathogenic yeast Candida albicans, because homozygous mutants of this diploid fungus can now be generated in a single step, allowing ...
Bernardo Ramírez-Zavala, Anna Hoffmann, Ines Krüger, Sonja Schwanfelder, Katherine S. Barker, P. David Rogers, Joachim Morschhäuser   +6 more
doaj   +1 more source

Global gene expression during stringent response in Corynebacterium glutamicum in presence and absence of the rel gene encoding (p) ppGpp synthase [PDF]

, 2006
Brockmann-Gretza O, Kalinowski J. Global gene expression during stringent response in Corynebacterium glutamicum in presence and absence of the rel gene encoding (p) ppGpp synthase. BMC Genomics.
Brockmann-Gretza Olaf, Kalinowski Jörn, Kalinowski, Jörn, Brockmann-Gretza, Olaf, Olaf Brockmann-Gretza, Jörn Kalinowski   +5 more
core   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Investigating GJB2 Mutation in 31 Individuals With Non-syndromic Hearing Loss

Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk, 2021
Background and Aim: Non-syndromic hearing loss is a genetically heterogeneous disorder. Mutation in the GJB2 gene is a major cause of non-syndromic hearing loss in numerous countries. This study aimed to evaluate GJB2 mutations in 31 individuals with non-
Pedram Pouryari Biyachal, Najmeh Ranji, Ali Nazemi   +2 more
doaj  

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

An Initial Genome Editing Toolset for Caldimonas thermodepolymerans, the First Model of Thermophilic Polyhydroxyalkanoates Producer

Microbial Biotechnology
The limited number of well‐characterised model bacteria cannot address all the challenges in a circular bioeconomy. Therefore, there is a growing demand for new production strains with enhanced resistance to extreme conditions, versatile metabolic ...
Anastasiia Grybchuk‐Ieremenko, Kristýna Lipovská, Xenie Kouřilová, Stanislav Obruča, Pavel Dvořák   +4 more
doaj   +1 more source

Conditional and constitutive expression of a Tbx1-GFP fusion protein in mice. [PDF]

, 2013
BACKGROUND: Velo-cardio-facial syndrome/DiGeorge syndrome (VCFS/DGS) is caused by a 1.5-3 Mb microdeletion of chromosome 22q11.2, frequently referred to as 22q11.2 deletion syndrome (22q11DS).
Morrow BE, Nowotschin, Sonja, Pirity MK, Baldini, Antonio, Freyer L, Freyer, Laina, Nowotschin S, Pirity, Melinda K., Morrow, Bernice E.   +8 more
core   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Clinical manifestations of Deletion 22q11.2 syndrome (DiGeorge/Velo-CardioFacial syndrome) [PDF]

, 2005
Deletion 22q11.2 syndrome (Del22) (DiGeorge/Velo-Cardio-Facial syndrome) is characterized by congenital heart defect (CHD), palatal anomalies, facial dysmorphisms, neonatal hypocalcemia, immune deficit, speech and learning disabilities. CHD is present
Marino, Bonnie, Capolino, Rossella, Digilio, Maria Cristina, Dallapiccola, B.   +3 more
core  

Off-Target Effects of Psychoactive Drugs Revealed by Genome-Wide Assays in Yeast [PDF]

, 2008
To better understand off-target effects of widely prescribed psychoactive drugs, we performed a comprehensive series of chemogenomic screens using the budding yeast Saccharomyces cerevisiae as a model system.
Nislow, Corey, Jan Wildenhain, Giaever, Guri, Guri Giaever, Gebbia, Marinella, Heisler Lawrence E., Tyers Mike, Ericson, Elke, Nislow Corey, Wildenhain, Jan, Corey Nislow, Elke Ericson, Wildenhain Jan, Lawrence E Heisler, Ericson Elke, Gebbia Marinella, Mike Tyers, Marinella Gebbia, Heisler, Lawrence E., Tyers, Mike, Giaever Guri   +20 more
core   +2 more sources