Results 81 to 90 of about 3,751,690 (317)

Ironing out the conflicts: iron supplementation reduces negatives bacterial interactions in the rhizosphere of an Atacama-endemic perennial grass

Environmental Microbiome
Background In plants, root exudates selectively influence the growth of bacteria that colonize the rhizosphere. Bacterial communities associated with root systems are involved in macro and micronutrients cycling and organic matter transformation.
Constanza Aguado-Norese, Jonathan E. Maldonado, Christian Hodar, Gabriel Galvez, Daniel E. Palma, Verónica Cambiazo, Mauricio Gonzalez   +6 more
doaj   +1 more source

Post-transcriptional gene silencing mediated by microRNAs is controlled by nucleoplasmic Sfpq

Nature Communications, 2017
There is a growing body of evidence about the presence and the activity of the miRISC in the nucleus of mammalian cells. Here, we show by quantitative proteomic analysis that Ago2 interacts with the nucleoplasmic protein Sfpq in an RNA-dependent fashion.
Silvia Bottini, Nedra Hamouda-Tekaya, Raphael Mategot, Laure-Emmanuelle Zaragosi, Stephane Audebert, Sabrina Pisano, Valerie Grandjean, Claire Mauduit, Mohamed Benahmed, Pascal Barbry, Emanuela Repetto, Michele Trabucchi   +11 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Deciphering DEL pocket patterns through contrastive learning

Nature Communications
DNA-encoded libraries (DELs) facilitate high-throughput screening of trillions of molecules against protein targets through split-pool synthesis and DNA tagging.
Wenyi Zhang, Yuxing Wang, Rui Zhan, Runtong Qian, Qi Hu, Jing Huang   +5 more
doaj   +1 more source

Sorting and packaging of RNA into extracellular vesicles shape intracellular transcript levels

BMC Biology, 2022
Background Extracellular vesicles (EVs) are released by nearly every cell type and have attracted much attention for their ability to transfer protein and diverse RNA species from donor to recipient cells.
Tina O’Grady, Makon-Sébastien Njock, Michelle Lion, Jonathan Bruyr, Emeline Mariavelle, Bartimée Galvan, Amandine Boeckx, Ingrid Struman, Franck Dequiedt   +8 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

An extended Kalman filtering approach to modeling nonlinear dynamic gene regulatory networks via short gene expression time series

, 2009
Copyright [2009] IEEE. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of Brunel University's products or services.
Liu, Y, Liu, X, Wang, Z, Liang, Y, Vinciotti, V   +4 more
core   +1 more source

The genomics of gene expression

Genomics, 2004
The study of gene regulation on a genomic scale has been constrained by the modest pace with which new trans-regulatory factors have been identified and by the fact that cis-regulatory sequences have to date been described even in part for only a small fraction of vertebrate genes.
openaire   +2 more sources

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene (MDR1) can sustain and enhance transgene expression in vivo [PDF]

, 2004
In gene therapy, a clinically relevant therapeutic effect requires long-term expression of the desired gene at a level sufficient to correct or at least alleviate the underlying gene defect.
Pfützner, Wolfgang, Vogel, Jonathan C.
core   +1 more source