Results 101 to 110 of about 658,370 (310)

TetR Regulated in vivo Repression Technology to Identify Conditional Gene Silencing in Genetically Engineerable Bacteria Using Vibrio cholerae Murine Infections as Model System

Bio-Protocol, 2020
Investigation of bacterial gene regulation upon environmental changes is still a challenging task. For example, Vibrio cholerae, a pathogen of the human gastrointestinal tract, faces diverse transient conditions in different compartments upon oral ...
Franz Zingl, Fabian Mitterer, Himadri Thapa, Stefan Schild   +3 more
doaj   +1 more source

Novel applications of shotgun phage display [PDF]

, 2004
In a shotgun phage display library, theoretically, the entire proteome of a bacterium is represented. Phages displaying specific polypeptides can be isolated by affinity selection, while the corresponding gene remains physically linked to the gene ...
Rosander, Anna
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Novel cyclic di-GMP effectors of the YajQ protein family control bacterial virulence [PDF]

, 2014
Bis-(3 ',5 ') cyclic di-guanylate (cyclic di-GMP) is a key bacterial second messenger that is implicated in the regulation of many critical processes that include motility, biofilm formation and virulence.
A Teplyakov, C Momany, C Saveanu, CD Boyd, CR Guzzo, D-G Ha, David Mackey, Delphine L. Caly, F Tao, GG Anderson, H Mulcahy, H Slater, H Sondermann, IS Pultz, J Duevel, J Mansfield, J Nesper, J. Maxwell Dow, Joseph Ward, K-H Chin, KB Twomey, Melanie Febrer, MY Galperin, R Hengge, Robert P. Ryan, RP Ryan, RP Ryan, RP Ryan, RP Ryan, RP Ryan, RP Ryan, S Moreau-Marquis, S-Q An, S-Q An, S-Q An, Sarah L. Murdoch, SE Maddocks, Shi-qi An, T Lundback, T Schirmer, U Romling, X Qiao, X-H Lu, Y Fouhy, Y McCarthy, Y McCarthy, Yvonne McCarthy   +46 more
core   +5 more sources

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Saccharomyces boulardii CNCM I-745 Stimulates Intracellular Antimicrobial Activity Against Salmonella Typhimurium in Murine Macrophages

Microorganisms
Salmonella enterica serovar Typhimurium (ST) is an intracellular pathogen that survives within macrophages and disseminates to systemic organs, thereby evading host immune defenses.
Rodolphe Pontier-Bres, Dorota Czerucka
doaj   +1 more source

Tissue Tropism in Host Transcriptional Response to Members of the Bovine Respiratory Disease Complex. [PDF]

, 2017
Bovine respiratory disease (BRD) is the most common infectious disease of beef and dairy cattle and is characterized by a complex infectious etiology that includes a variety of viral and bacterial pathogens.
Behura, Susanta K, Gershwin, Laurel J, Grupioni, Natalia V, Kim, JaeWoo, Neibergs, Holly L, Regitano, Luciana CA, Schnabel, Robert D, Seabury, Christopher M, Taylor, Jeremy F, Tizioto, Polyana C, Toaff-Rosenstein, Rachel, Van Eenennaam, Alison L   +11 more
core   +3 more sources

Regulation of bacterial gene expression by the NTP substrates of transcription initiation [PDF]

Molecular Microbiology, 2008
SummaryMany mechanisms of gene regulation in bacteria do not employ repressor or activator proteins. One class of these mechanisms includes those in which the key regulatory element is the control of transcription initiation by the availability of NTP substrates.
openaire   +2 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source