Results 1 to 10 of about 6,261 (210)

Cycling of RNAs on Hfq [PDF]

RNA Biology, 2013
The RNA chaperone Hfq is a key player in small RNA (sRNA)-mediated regulation of target mRNAs in many bacteria. The absence of this protein causes pleiotropic phenotypes such as impaired stress regulation and, occasionally, loss of virulence.
Wagner, Gerhart,
core   +4 more sources

No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus

BMC Microbiology, 2007
Background The RNA-binding protein Hfq is involved in stress and virulence of several pathogens, probably due to its role as mediator in small RNA (sRNA)-mRNA interactions.
Bouloc Philippe, Rigoulay Candice, Bohn Chantal   +2 more
doaj   +1 more source

Structural and biochemical studies on ATP binding and hydrolysis by the Escherichia coli RNA chaperone Hfq. [PDF]

PLoS ONE, 2012
In Escherichia coli the RNA chaperone Hfq is involved in riboregulation by assisting base-pairing between small regulatory RNAs (sRNAs) and mRNA targets. Several structural and biochemical studies revealed RNA binding sites on either surface of the donut
Hermann Hämmerle, Mads Beich-Frandsen, Branislav Večerek, Lukas Rajkowitsch, Oliviero Carugo, Kristina Djinović-Carugo, Udo Bläsi   +6 more
doaj   +1 more source

Study of E. coli Hfq's RNA annealing acceleration and duplex destabilization activities using substrates with different GC-contents

, 2012
Folding of RNA molecules into their functional three-dimensional structures is often supported by RNA chaperones, some of which can catalyse the two elementary reactions helix disruption and helix formation.
Saxena, Krishna, Doetsch, Martina, Stampfl, Sabine, Beich-Frandsen, Mads, Lybecker, Meghan, Fürtig, Boris, Schroeder, Renée   +6 more
core   +1 more source

Hfq- and sRNA-mediated regulation in Neisseria meningitidis

, 2021
Neisseria meningitidis, also known as the meningococcus, is a human-specific pathogen that commonly colonises the nasopharynx without causing disease. For reasons unknown, N.
Jens Karlsson (18677893)
core  

Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri

BMC Microbiology, 2019
Background Hfq is a widely conserved bacterial RNA-binding protein which generally mediates the global regulatory activities involv ed in physiological process and virulence.
Xuelu Liu, Yuping Yan, Haodi Wu, Changyong Zhou, Xuefeng Wang   +4 more
doaj   +1 more source

Amyloid-like Hfq interaction with single-stranded DNA: involvement in recombination and replication in Escherichia coli

QRB Discovery, 2022
Interactions between proteins and single-stranded DNA (ssDNA) are crucial for many fundamental biological processes, including DNA replication and genetic recombination.
Krzysztof Kubiak, Frank Wien, Indresh Yadav, Nykola C. Jones, Søren Vrønning Hoffmann, Eric Le Cam, Antoine Cossa, Frederic Geinguenaud, Johan R. C. van der Maarel, Grzegorz Węgrzyn, Véronique Arluison   +10 more
doaj   +1 more source

Expression of Hfq-MT protein.

, 2013
Hfq-MT construct (A). The pBAD-Hfq-MT plasmid allowing the expression of Hfq-MT under the control of the arabinose pBAD promoter. Semi-quantitative Western Blot (B) Relative quantification of intracellular Hfq was achieved by using crude extracts of (a ...
Cristina Risco (368646), Elia Diestra (368640), Bastien Cayrol (368642), Véronique Arluison (25027)   +3 more
core   +1 more source

Shewanella oneidensis Hfq promotes exponential phase growth, stationary phase culture density, and cell survival

BMC Microbiology, 2013
Background Hfq is an RNA chaperone protein that has been broadly implicated in sRNA function in bacteria. Here we describe the construction and characterization of a null allele of the gene that encodes the RNA chaperone Hfq in Shewanella oneidensis ...
Brennan Christopher M, Keane Meghan L, Hunt Taylor M, Goulet Matthew T, Mazzucca Nicholas Q, Sexton Zachary, Mezoian Taylor, Douglas Katherine E, Osborn Jessica M, Pellock Brett J   +9 more
doaj   +1 more source

Reduced heme levels underlie the exponential growth defect of the Shewanella oneidensis hfq mutant. [PDF]

PLoS ONE, 2014
The RNA chaperone Hfq fulfills important roles in small regulatory RNA (sRNA) function in many bacteria. Loss of Hfq in the dissimilatory metal reducing bacterium Shewanella oneidensis strain MR-1 results in slow exponential phase growth and a reduced ...
Christopher M Brennan, Nicholas Q Mazzucca, Taylor Mezoian, Taylor M Hunt, Meaghan L Keane, Jessica N Leonard, Shelby E Scola, Emma N Beer, Sarah Perdue, Brett J Pellock   +9 more
doaj   +1 more source