RNAs Associated With Bacterial Outer Membrane Vesicles: Structural Insights Into Surface Composition. [PDF]
J Extracell VesiclesThis work uncovers a new layer of bacterial communication. We show that RNAs, aided by the Hfq RNA‐chaperone, are not only packed inside Gram negative Outer Membrane Vesicles (OMVs) but can also be embedded in their membranes. This suggests that bacteria may use surface‐exposed RNAs to directly interact with host cells. Overall, our results broaden the
Mosca K +4 more
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De Novo Discovery of Bioactive Cyclic Peptides via Cellular Selection of Genetically Encoded Libraries. [PDF]
ChembiochemGenetically encoded cyclic peptide libraries can be employed in cellular selection platforms to identify cyclic peptide inhibitors for therapeutic targets by directly selecting for desired biological activities. This review systematically summarizes representative studies and suggests future opportunities for using cellular selection for de novo cyclic
Yang L, Behera A, Meyers HJ, Wang T.
europepmc +2 more sources
Uncovering a previously unknown function of polyphosphate in polyadenylated RNA-induced amyloidogenesis of Hfq. [PDF]
FEBS JPolyphosphate interaction with Hfq and RNA drives phase separation and amyloid self‐assembly, uncovering a previously unrecognized role for polyP in bacterial stress responses. Our findings reveal new insights into the molecular mechanisms of RNA‐mediated phase separation, highlighting the role of polyP as a key modulator of this process. Polyphosphate
Mosca K +5 more
europepmc +2 more sources
Pervasive Targeting of Nascent Transcripts by Hfq
Cell Reports, 2018 Summary: Hfq is an RNA chaperone and an important post-transcriptional regulator in bacteria. Using chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq), we show that Hfq associates with hundreds of different regions of ...
Simon L Dove, Kathryn M Ramsey
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The RNA chaperone Hfq is involved in stress tolerance and virulence in uropathogenic Proteus mirabilis. [PDF]
PLoS ONE, 2014 Hfq is a bacterial RNA chaperone involved in the riboregulation of diverse genes via small noncoding RNAs. Here, we show that Hfq is critical for the uropathogenic Proteus mirabilis to effectively colonize the bladder and kidneys in a murine urinary ...
Min-Cheng Wang +4 more
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The RNA chaperone Hfq impacts growth, metabolism and production of virulence factors in Yersinia enterocolitica. [PDF]
PLoS ONE, 2014 To adapt to changes in environmental conditions, bacteria regulate their gene expression at the transcriptional but also at the post-transcriptional level, e.g. by small RNAs (sRNAs) which modulate mRNA stability and translation.
Tamara Kakoschke +6 more
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444 Post-translational role of RNA modifications in sRNA chaperone Hfq
Journal of Clinical and Translational Science, 2022 OBJECTIVES/GOALS: The goal of this study is to determine the role of the tRNA modifications in the translation of Hfq. Hfq is an RNA chaperone that acts as a co-factor for the action of the largest class of small RNAs in E. coli.
Jalisa Nurse +2 more
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Hfq is a global regulator that controls the pathogenicity of Staphylococcus aureus. [PDF]
PLoS ONE, 2010 The Hfq protein is reported to be an RNA chaperone, which is involved in the stress response and the virulence of several pathogens. In E. coli, Hfq can mediate the interaction between some sRNAs and their target mRNAs.
Yu Liu +7 more
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eLife, 2021 RNA-binding proteins play myriad roles in regulating RNAs and RNA-mediated functions. In bacteria, the RNA chaperone Hfq is an important post-transcriptional gene regulator.
Seongjin Park +8 more
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Profound impact of Hfq on nutrient acquisition, metabolism and motility in the plant pathogen Agrobacterium tumefaciens. [PDF]
PLoS ONE, 2014 As matchmaker between mRNA and sRNA interactions, the RNA chaperone Hfq plays a key role in riboregulation of many bacteria. Often, the global influence of Hfq on the transcriptome is reflected by substantially altered proteomes and pleiotropic ...
Philip Möller +6 more
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