Communications Biology, 2023 Hfq is a pleitropic actor that serves as stress response and virulence factor in the bacterial cell. To execute its multiple functions, Hfq assembles into symmetric torus-shaped hexamers.
Mélanie Berbon +8 more
doaj +2 more sources
Models of Hfq interactions with small non-coding RNA in Gram-negative and Gram-positive bacteria
Frontiers in Cellular and Infection Microbiology, 2023 Hfq is required by many Gram-negative bacteria to chaperone the interaction between small non-coding RNA (sRNA) and mRNA to facilitate annealing. Conversely and despite the presence of Hfq in many Gram-positive bacteria, sRNAs in Gram-positive bacteria ...
Derrick Watkins, Dev Arya
doaj +2 more sources
Condensation of the RNA chaperone Hfq is coupled to inhibition of glucose uptake and contributes to the stabilization of regulatory RNAs in nitrogen-starved Escherichia coli. [PDF]
Nucleic Acids ResRibonucleoprotein condensates are membraneless compartments that concentrate RNA-binding proteins and RNA, and play key roles in cellular adaptation across both eukaryotes and bacteria.
McQuail J +6 more
europepmc +2 more sources
Small RNAs and Hfq capture unfolded RNA target sites during transcription
Molecular Cell, 2023 SUMMARY Small ribonucleoproteins (sRNPs) target nascent precursor RNAs to guide folding, modification, and splicing during transcription. Yet, rapid co-transcriptional folding of the RNA can mask sRNP sites, impeding target recognition and regulation. To
Margaret L Rodgers, Sarah A Woodson
exaly +2 more sources
Uncovering a previously unknown function of polyphosphate in polyadenylated RNA-induced amyloidogenesis of Hfq. [PDF]
FEBS JPolyphosphate interaction with Hfq and RNA drives phase separation and amyloid self‐assembly, uncovering a previously unrecognized role for polyP in bacterial stress responses. Our findings reveal new insights into the molecular mechanisms of RNA‐mediated phase separation, highlighting the role of polyP as a key modulator of this process. Polyphosphate
Mosca K +5 more
europepmc +2 more sources
Hfq balances energetic efficiency and antibiotic persistence in <i>Acinetobacter baumannii</i>. [PDF]
mSystemsThe RNA chaperone Hfq of Acinetobacter baumannii has been documented in altering antibiotic susceptibility and other optimal stress tolerance capabilities of this pathogen.
Sett A +4 more
europepmc +2 more sources
The Pleiotropic Phenotypes Caused by an hfq Null Mutation in Vibrio harveyi
Microorganisms, 2023 Hfq is a global regulator and can be involved in multiple cellular processes by assisting small regulatory RNAs (sRNAs) to target mRNAs. To gain insight into the virulence regulation of Hfq in Vibrio harveyi, the hfq null mutant, ∆hfq, was constructed in
Yiqin Deng +5 more
doaj +2 more sources
Heterotypic phase separation of Hfq is linked to its roles as an RNA chaperone
Cell Reports, 2022 Hfq, an Sm-like protein and the major RNA chaperone in E. coli, has been shown to distribute non-uniformly along a helical path under normal growth conditions and to relocate to the cell poles under certain stress conditions.
Orna Amster-Choder, Tamar Szöke
exaly +2 more sources
Competition for Hfq drives kinetic selection of mRNA targets by <i>Escherichia coli</i> small noncoding RNAs. [PDF]
Proc Natl Acad Sci U S ASmall non-coding RNAs (sRNAs) regulate gene expression in E. coli bacteria in response to diverse stimuli in the environment and the host. Most sRNAs regulate mRNA expression by directly base pairing with complementary sites in the target mRNA with the ...
Roca J, Chen YL, Woodson SA.
europepmc +2 more sources
mSphere, 2023 The RNA chaperone Hfq acts as a global regulator of numerous biological processes, such as carbon/nitrogen metabolism and environmental adaptation in plant-associated diazotrophs; however, its target RNAs and the mechanisms underlying nitrogen fixation ...
Fanyang Lv +13 more
doaj +2 more sources