Results 151 to 160 of about 779,265 (203)
Some of the next articles are maybe not open access.
Bacterial lipases from Pseudomonas: regulation of gene expression and mechanisms of secretion.
Biochimie, 2000Lipases from Pseudomonas bacteria are widely used for a variety of biotechnological applications. Overexpression in heterologous hosts like Escherichia coli failed to produce enzymatically active lipase prompting to study the molecular mechanisms underlying the regulation of lipase gene expression and secretion. The prototype lipase from P.
F. Rosenau, Karl-Erich Jaeger
semanticscholar +3 more sources
CRISPR Interference in Regulation of Bacterial Gene Expression
Molecular Biology, 2022The review is devoted to the use of the CRISPR/Cas system for obtaining knockdowns of target bacterial genes by CRISPR-mediated interference (CRISPRi). CRISPRi is based on the preservation of the ability of the inactivated dCas nuclease in complex with guide RNA to bind a target, which leads to reversible repression of the selected genes.
N I, Nadolinskaia, A V, Goncharenko
openaire +2 more sources
Temperature-regulated expression of bacterial virulence genes
Microbes and Infection, 2000Virulence gene expression in most bacteria is a highly regulated phenomenon, affected by a variety of parameters including osmolarity, pH, ion concentration, iron levels, growth phase, and population density. Virulence genes are also regulated by temperature, which acts as an 'on-off' switch in a manner distinct from the more general heat-shock ...
M E, Konkel, K, Tilly
openaire +2 more sources
Crystallization of Hfq protein: a bacterial gene-expression regulator
Acta Crystallographica Section D Biological Crystallography, 2003Hfq protein from Escherichia coli (EcoHfq) has been overproduced in E. coli, purified to homogeneity and crystallized using the hanging-drop vapour-diffusion technique. Crystallization conditions for EcoHfq were found which yielded X-ray quality crystals.
Vassilieva, Ioulia M. +4 more
openaire +3 more sources
Noise in bacterial gene expression.
Biochemical Society Transactions, 2019The expression level of a gene can fluctuate significantly between individuals within a population of genetically identical cells. The resultant phenotypic heterogeneity could be exploited by bacteria to adapt to changing environmental conditions.
C. Engl
semanticscholar +1 more source
Bacterial expression system with tightly regulated gene expression and plasmid copy number
Gene, 2004A new Escherichia coli host/vector system has been engineered to allow tight and uniform modulation of gene expression and gamma origin (ori) plasmid copy number. Regulation of gamma ori plasmid copy number is achieved through arabinose-inducible expression of the necessary Rep protein, pi, whose gene was integrated into the chromosome of the host ...
Lisa M, Bowers +4 more
openaire +2 more sources
Regulation of bacterial gene expression by ribosome stalling and rescuing
Current Genetics, 2015Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing.
Yongxin, Jin, Shouguang, Jin, Weihui, Wu
openaire +2 more sources
Metal Regulation of Gene Expression in Bacterial Systems
1998Metals are important in biochemical processes (da Silva and Williams, 1991). They can be cofactors of enzymatic reactions or they can be the key redox components of electron transport processes. Zinc is an example of a metal whose properties as a Lewis acid are used in the reactions of a wide variety of catalytic processes, and a quick glance through a
Nigel L. Brown +3 more
openaire +1 more source
Regulation of Bacterial Gene Expression by Protease-Alleviated Spatial Sequestration (PASS)
ACS Synthetic Biology, 2015In natural microbial systems, conditional spatial sequestration of transcription factors enables cells to respond rapidly to changes in their environment or intracellular state by releasing presynthesized regulatory proteins. Although such a mechanism may be useful for engineering synthetic biology technologies ranging from cell-based biosensors to ...
Ragan A, Pitner +2 more
openaire +2 more sources
Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression
Nature, 2002Although proteins fulfil most of the requirements that biology has for structural and functional components such as enzymes and receptors, RNA can also serve in these capacities. For example, RNA has sufficient structural plasticity to form ribozyme and receptor elements that exhibit considerable enzymatic power and binding specificity. Moreover, these
Wade, Winkler +2 more
openaire +2 more sources