Results 11 to 20 of about 282,788 (245)

Correction of gene model annotations improves isoform abundance estimates: the example of ketohexokinase (Khk) [version 2; peer review: 2 approved, 1 approved with reservations]

F1000Research, 2019
Next generation sequencing protocols such as RNA-seq have made the genome-wide characterization of the transcriptome a crucial part of many research projects in biology.
Christophe D. Chabbert, Tanja Eberhart, Ilaria Guccini, Wilhelm Krek, Werner J. Kovacs   +4 more
doaj   +1 more source

Isoform switch of pyruvate kinase M1 indeed occurs but not to pyruvate kinase M2 in human tumorigenesis. [PDF]

PLoS ONE, 2015
Muscle type of pyruvate kinase (PKM) is one of the key mediators of the Warburg effect and tumor metabolism. Due to alternative splicing, there are at least 12 known isoforms of the PKM gene, of which PKM1 and PKM2 are two major isoforms with only a 23 ...
Cheng Zhan, Li Yan, Lin Wang, Jun Ma, Wei Jiang, Yongxing Zhang, Yu Shi, Qun Wang   +7 more
doaj   +1 more source

The p52 isoform of SHC1 is a key driver of breast cancer initiation

Breast Cancer Research, 2019
Background SHC1 proteins (also called SHCA) exist in three functionally distinct isoforms (p46SHC, p52SHC, and p66SHC) that serve as intracellular adaptors for several key signaling pathways in breast cancer.
Kevin D. Wright, Bradley S. Miller, Sarah El-Meanawy, Shirng-Wern Tsaih, Anjishnu Banerjee, Aron M. Geurts, Yuri Sheinin, Yunguang Sun, Balaraman Kalyanaraman, Hallgeir Rui, Michael J. Flister, Andrey Sorokin   +11 more
doaj   +1 more source

Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycle

Nature Communications, 2021
H2A.X histone variant gene encodes poly(A)+ and poly(A)- mRNA isoforms which are differentially expressed depending on cell lines. Here the authors show that upon DNA damage, cells expressing more poly(A)+ isoform require this isoform for de novo H2A.X ...
Esther Griesbach, Margarita Schlackow, William F. Marzluff, Nick J. Proudfoot   +3 more
doaj   +1 more source

Systematically differentiating functions for alternatively spliced isoforms through integrating RNA-seq data.

PLoS Computational Biology, 2013
Integrating large-scale functional genomic data has significantly accelerated our understanding of gene functions. However, no algorithm has been developed to differentiate functions for isoforms of the same gene using high-throughput genomic data.
Ridvan Eksi, Hong-Dong Li, Rajasree Menon, Yuchen Wen, Gilbert S Omenn, Matthias Kretzler, Yuanfang Guan   +6 more
doaj   +1 more source

The loss of DLG2 isoform 7/8, but not isoform 2, is critical in advanced staged neuroblastoma

Cancer Cell International, 2021
Background Neuroblastoma is a childhood neural crest tumor showing large clinical and genetic heterogeneity, one form displaying 11q-deletion is very aggressive. It has been shown that 11q-deletion results in decreased expression of DLG2, a gene residing
Simon Keane, Tommy Martinsson, Per Kogner, Katarina Ejeskär   +3 more
doaj   +1 more source

Obstacles to detecting isoforms using full-length scRNA-seq data

Genome Biology, 2020
Background Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual to see more than one isoform being produced from a gene in a single cell, even when multiple isoforms were detected in matched bulk RNA-seq samples.
Jennifer Westoby, Pavel Artemov, Martin Hemberg, Anne Ferguson-Smith   +3 more
doaj   +1 more source

Alternative Translation Initiation Generates a Functionally Distinct Isoform of the Stress-Activated Protein Kinase MK2

Cell Reports, 2019
Summary: Alternative translation is an important mechanism of post-transcriptional gene regulation leading to the expression of different protein isoforms originating from the same mRNA.
Philipp Trulley, Goda Snieckute, Dorte Bekker-Jensen, Manoj B. Menon, Robert Freund, Alexey Kotlyarov, Jesper V. Olsen, Manuel D. Diaz-Muñoz, Martin Turner, Simon Bekker-Jensen, Matthias Gaestel, Christopher Tiedje   +11 more
doaj   +1 more source

HNF4α Combinatorial Isoform Heterodimers Activate Distinct Gene Targets that Differ from Their Corresponding Homodimers

Cell Reports, 2019
Summary: Hepatocyte nuclear factor 4α (HNF4α) is a dimeric transcription factor that controls as much as 60% of all liver genes. However, how it achieves such broad functional diversity is unknown. Here, we show that inflammation and immune pathway genes
Hui Ling Ko, Ziyi Zhuo, Ee Chee Ren
doaj   +1 more source

Bovine C-X-C Motif Chemokine Ligand 14 Expression Is Regulated by Alternative Polyadenylation and MicroRNAs

Animals, 2023
Alternative polyadenylation (APA), including APA that occurs only in the 3′ UTR (3′ UTR-APA), is an important post-transcriptional regulatory mechanism that leads to distinct 3′ UTRs for some genes, increasing the complexity of the transcriptome.
Wei Zhao, Xueyan Liu, Chengping Li, Xuyong Qin, Shizhong Ren, Shujun Cao, Guoli Zhou   +6 more
doaj   +1 more source