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Targeted Gene Knockdown in Early Embryos Using siRNA
2017RNA interference is a convenient and highly effective technique to investigate the biological function of genes. Adequately designed RNA molecules introduced into an oocyte are able to bind specific endogenous mRNAs and trigger their degradation. Subsequent fertilization of these oocytes will result in the generation of embryos in which the expression ...
Lu, Zhang, Zoltan, Machaty
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Lentiviral Strategies for RNAi Knockdown of Neuronal Genes
Current Protocols in Neuroscience, 2007AbstractRNA interference (RNAi) refers to the process by which 21‐ to 23‐nucleotide short interfering RNAs (siRNAs) mediate post‐transcriptional degradation of homologous mRNA transcripts. This process is carried out by an endogenous pathway that centers on the use of endogenously encoded small RNAs, and can be hijacked to knock down the expression of ...
Jill R, Crittenden +2 more
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siRNA stabilization prolongs gene knockdown in primary T lymphocytes
European Journal of Immunology, 2008AbstractRNA interference (RNAi)‐mediated knockdown of target gene expression represents a powerful approach for functional genomics and therapeutic applications. However, for T lymphocytes, central regulators of immunity and immunopathologies, the application of RNAi has been limited due to the lack of efficient small interfering RNA (siRNA) delivery ...
Mantei, A. +10 more
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Antisense-Mediated Exon-Skipping to Induce Gene Knockdown
2012Exon-skipping antisense oligonucleotides (ASOs) can be used to knockdown the expression of an undesired gene or specific gene isoform. This chapter discusses the potential therapeutic applications of the technique and provides a sample protocol for inducing exon-skipping in Apolipoprotein B in vitro, as well as a protocol for quantifying exon-skipping ...
Petra, Disterer, Bernard, Khoo
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Efficient designs for multiple gene knockdown experiments
2010 IEEE International Workshop on Genomic Signal Processing and Statistics (GENSIPS), 2010This paper develops theoretical bounds on the number of required experiments to infer which genes are active in a particular biological process. The standard approach is to perform many experiments, each with a single gene suppressed or knocked down. However, certain effects are not revealed by single-gene knockouts and are only observed when two or ...
B Nazer, R D Nowak
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Gene Knockdown Analysis by Double-Stranded RNA Injection
2011The discovery of RNAi, in which double-stranded RNA (dsRNA) suppresses the translation of homologous mRNA, has had a huge impact on evolutionary genetics by enabling the analysis of loss-of-function phenotypes in organisms in which classical genetic analysis is laborious or impossible.
Benjamin N, Philip, Yoshinori, Tomoyasu
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RNA interference-mediated gene knockdown within specific cell types
Plant Molecular Biology, 2012In plants, RNA interference (RNAi)-induced gene silencing can spread from the initiation site to nearby cells. The silencing signal moves from cell-to-cell through plasmodesmata and, over long distances, through the phloem. In this study, we employed a nuclear-localized GFP fusion protein to visualize the pattern of gene silencing induced by three ...
Changqing, Zhang, David W, Galbraith
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A versatile tool for conditional gene expression and knockdown
Nature Methods, 2006Drug-inducible systems allowing the control of gene expression in mammalian cells are invaluable tools for genetic research, and could also fulfill essential roles in gene- and cell-based therapy. Currently available systems, however, often have limited in vivo functionality because of leakiness, insufficient levels of induction, lack of tissue ...
Jolanta, Szulc +4 more
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siRNA Knockdown of Gene Expression in Endothelial Cells
2011Cultured endothelial cells are renowned for being difficult to transfect, whether for the purpose of exogenous over-expression of plasmid DNA or for genetic knockdown via silencing RNA. Therefore, optimal conditions are absolutely necessary for achieving relatively high transfection efficiency coupled with low cellular toxicity in endothelial cells ...
Emily, Dennstedt, Brad, Bryan
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Probing the nanoparticle–AGO2 interaction for enhanced gene knockdown
Soft Matter, 2018RNA interference is a promising technology for treatment of various diseases. Here, we systematically probe the effect of steric hindrance of nanoparticles on the RNA induced silencing complex (RISC) interaction, by modulating two parameters, the nanoparticle size and hardness.
Sonal Deshpande, Neetu Singh
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