Results 41 to 50 of about 405,164 (304)
PharmaceuticsBackground: Polymeric nanoparticles have been explored as efficient tools for siRNA delivery to induce RNAi-mediated gene knockdown. Chemical modifications of polyethylenimines (PEI) enhance nanoparticle efficacy and biocompatibility.
Sandra Noske +3 more
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Quantitative PCR primer design affects quantification of dsRNA‐mediated gene knockdown
Ecology and Evolution, 2019 RNA interference (RNAi) is a powerful tool for studying functions of candidate genes in both model and nonmodel organisms and a promising technique for therapeutic applications.
Thomas Ogao Onchuru, Martin Kaltenpoth
doaj +1 more source
PARP1 gene expression is downregulated by knockdown of PARG gene
Oncology Reports, 2013 Poly(ADP-ribosyl)ation is a modification of nuclear proteins that regulates DNA replication, repair and transcription. In order to investigate the biological effects of degradation of poly(ADP-ribose), knockdown of the poly(ADP-ribose) glycohydrolase (PARG) gene was performed by introducing a short interfering RNA (siRNA)-pool into HeLa S3 cells ...
UCHIUMI, FUMIAKI +4 more
openaire +3 more sources
CRISPR/Cas9-mediated gene knock-down in post-mitotic neurons. [PDF]
PLoS ONE, 2014 The prokaryotic adaptive immune system CRISPR/Cas9 has recently been adapted for genome editing in eukaryotic cells. This technique allows for sequence-specific induction of double-strand breaks in genomic DNA of individual cells, effectively resulting ...
Christoph Straub +3 more
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Enhancing Gene-Knockdown Efficiency of Poly(N-isopropylacrylamide) Nanogels [PDF]
ACS Omega, 2018 Polo-like-kinase 1 (PLK1), which is a serine-threonine protein kinase overexpressed in cancer cells, is known to regulate tumor growth and have recently gathered attention as a target gene for RNA interference because of the poor bioavailability and nonspecificity of the available inhibitors.
Sonal Deshpande +2 more
openaire +3 more sources
Pharmaceutics, 2019 In this study, we examined the effect of cationic lipid type in folate (FA)-polyethylene glycol (PEG)-modified cationic liposomes on gene-silencing effects in tumor cells using cationic liposomes/siRNA complexes (siRNA lipoplexes). We used three types of
Yoshiyuki Hattori +3 more
doaj +1 more source
The role and implications of mammalian cellular circadian entrainment
FEBS Letters, EarlyView.At their most fundamental level, mammalian circadian rhythms occur inside every individual cell. To tell the correct time, cells must align (or ‘entrain’) their circadian rhythm to the external environment. In this review, we highlight how cells entrain to the major circadian cues of light, feeding and temperature, and the implications this has for our
Priya Crosby
wiley +1 more source
Frontiers in Microbiology, 2020 Cilia are highly conserved organelles present in almost all types of eukaryotic cells, and defects in cilia structure and/or function are related to many human genetic disorders.
Danxu Tang +14 more
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Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions
FEBS Letters, EarlyView.Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf +6 more
wiley +1 more source
Frontiers in EndocrinologyIntroductionMro-IR is an insulin-like receptor and is uniquely expressed in the ovary of the freshwater giant prawn Macrobrachium rosenbergii. However, the understanding of this conserved receptor involved in the molecular mechanism underpinning ovarian ...
Wen-Ming Ma, Hai-Jing Xu
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