Mobile-CRISPRi as a tool for genetic manipulation in the intracellular pathogen Piscirickettsia salmonis [PDF]
Applied and Environmental MicrobiologyPiscirickettsia salmonis is the causative agent of salmonid rickettsial septicemia (SRS), the main bacterial disease affecting the salmon industry in Chile.
Javiera Ortiz-Severin +6 more
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Addressing the challenges of symbiont-mediated RNAi in aphids [PDF]
PeerJ, 2023 Because aphids are global agricultural pests and models for bacterial endosymbiosis, there is a need for reliable methods to study and control their gene function.
Katherine M. Elston +3 more
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CRISPR Toolbox for Genome Editing in Dictyostelium
Frontiers in Cell and Developmental Biology, 2021 The development of new techniques to create gene knockouts and knock-ins is essential for successful investigation of gene functions and elucidation of the causes of diseases and their associated fundamental cellular processes.
Kensuke Yamashita +3 more
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Using siRNA Technique to Generate Transgenic Animals with Spatiotemporal and Conditional Gene Knockdown [PDF]
The American Journal of Pathology, 2004 Based on the RNAi technique, we have developed a new approach that generates transgenic animals capable of mimicking human genetic diseases. The new system is a combination of siRNA with Cre-loxP and tetracycline-on. It has the characteristics of being stable, inheritable, and inducible, with the siRNA able to be transcribed tissue specifically.
Hung-Shu, Chang +3 more
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siRNA-mediated gene knockdown via electroporation in hydrozoan jellyfish embryos
Scientific Reports, 2022 As the sister group to bilaterians, cnidarians stand in a unique phylogenetic position that provides insight into evolutionary aspects of animal development, physiology, and behavior.
Tokiha Masuda-Ozawa +5 more
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FGF19 promotes cell autophagy and cisplatin chemoresistance by activating MAPK signaling in ovarian cancer [PDF]
PeerJ, 2023 Background Chemotherapy is one of the primary treatments for ovarian cancer patients. Autophagy has been linked to chemotherapy resistance in tumor cells. Recent studies have suggested that fibroblast growth factor 19 (FGF19) may be involved in the onset
Wei Zhu +4 more
doaj +2 more sources
iScience, 2023 Summary: Evidence is mounting for cross-resistance between immune checkpoint and targeted kinase inhibitor therapies in cutaneous melanoma patients. Since the loss of the transcription factor, SOX10, causes tolerance to MAPK pathway inhibitors, we used ...
Timothy J. Purwin +4 more
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Development of the morpholino gene knockdown technique in Fundulus heteroclitus: A tool for studying molecular mechanisms in an established environmental model [PDF]
Aquatic Toxicology, 2008 A significant challenge in environmental toxicology is that many genetic and genomic tools available in laboratory models are not developed for commonly used environmental models. The Atlantic killifish (Fundulus heteroclitus) is one of the most studied teleost environmental models, yet few genetic or genomic tools have been developed for use in this ...
Matson, Cole W +5 more
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Validating the use of short interfering RNA as a novel technique for cell-specific target gene knockdown in lung ischemia–reperfusion injury [PDF]
The Journal of Thoracic and Cardiovascular Surgery, 2016 Short interfering RNA is an effective method for target gene knockdown. However, concerns surround the design, administration, efficacy, specificity, and immunostimulatory potential. Although uptake by alveolar macrophages has been demonstrated, studies have not examined its use in lung ischemia-reperfusion injury.
Heather E, Merry +3 more
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A functional genetic toolbox for human tissue-derived organoids
eLife, 2021 Human organoid systems recapitulate key features of organs offering platforms for modelling developmental biology and disease. Tissue-derived organoids have been widely used to study the impact of extrinsic niche factors on stem cells.
Dawei Sun +8 more
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