Results 221 to 230 of about 1,333,192 (304)

Additive‐Manufactured, Multifunctional Bioreactor Technology for Dynamic Culture of 3D Bioprinted Tissue Models

Advanced Materials Technologies, EarlyView.
We introduce an additively manufactured bioreactor with a perfusion flow system and integrated temperature and pH sensors for skeletal muscle tissue biofabrication. The bioreactor's performance was evaluated by assessing the viability, spreading of the myoblast cells in a printed scaffold, and contraction of the isolated murine musculi interossei ...
Lys Sprenger, Johann Schorzmann, Hsuan‐Heng Lu, Muhammad Osama, Julian Bauer, Michael Haug, Oliver Friedrich, Aldo R. Boccaccini, Frank Döpper, Sahar Salehi   +9 more
wiley   +1 more source

A cost-effective and scalable barcoded library construction method for deep mutational scanning studies. [PDF]

PLoS Biol
Jann J, Gagnon-Arsenault I, Pageau A, Dubé AK, Fijarczyk A, Durand R, Landry CR.   +6 more
europepmc   +1 more source

AI‐Powered Framework for Evaluating Drug Efficacy for Three‐Dimensional In Vitro Cancer Models in Robot‐Assisted Production

Advanced Robotics Research, EarlyView.
An AI‐powered, robot‐assisted framework automatically produces, images, and analyzes 3D tumor spheroids to evaluate drug efficacy. Integrated modules handle spheroid formation, live/dead staining, brightfield imaging, and automated image analysis, including spheroid segmentation, viability and metrics to assess the drug treatment efficacy. The workflow
Dalia Mahdy, Jesus Nieves, Lukas Königer, Matthias Peindl, Fabio Erhard, Marc Möllmann, Anke Klingner, Markus Münig, Bibin Babu, Thomas Schwarz, Marco Metzger, Gudrun Dandekar, Maximiliane Wußmann, Jan Hansmann   +13 more
wiley   +1 more source

Insights from pooled CRISPRi single-cell screens in K562 cells reveal gene functions, regulatory networks, and highlight opportunities and limitations. [PDF]

BMC Genomics
Zhang H, Zhang P, Bindels E, Mulugeta E.
europepmc   +1 more source

From Lab to Landscape: Environmental Biohybrid Robotics for Ecological Futures

Advanced Robotics Research, EarlyView.
This Perspective explores environmental biohybrid robotics, integrating living tissues, microorganisms, and insects for operation in real‐world ecosystems. It traces the leap from laboratory experiments to forests, wetlands, and urban environments and discusses key challenges, development pathways, and opportunities for ecological monitoring and ...
Miriam Filippi
wiley   +1 more source

Construction of a Normalized Library and Screening of Transcriptional Regulators of the cas5 Gene in Corynespora cassiicola. [PDF]

Microorganisms
Zhu B, Hu G, Yang Z, Khan RAA, Ahmad M, Khalid MZ, Liu T, Hou J.   +7 more
europepmc   +1 more source

Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation

Advanced Science, EarlyView.
Nuclear metabolism oscillates during cell cycle progression. Quantitative chromatome proteomics and imaging reveal phase‐specific dynamics of PIP5K1A and nuclear PIP2, linking phosphatidylinositol metabolism to histone methylation. This work identifies nuclear lipid metabolism as a previously unrecognized regulatory axis coordinating chromatin ...
Antoni Gañez‐Zapater, Savvas Kourtis, Camilla Reiter Elbæk, Lorena Espinar, Carolina Toro‐Márquez, Albert Coll‐Manzano, Alfredo Smiriglia, Laura García‐López, Laura Wiegand, Maria Guirola, Frédéric Fontaine, Andrea Morandi, André C. Müller, Sara Sdelci   +13 more
wiley   +1 more source

Systematic performance evaluation and application validation of an end-to-end NGS workstation. [PDF]

Sci Rep
Xie W, Yang C, Ren S.
europepmc   +1 more source

Chronic Hypoxia Disrupts Spermatogenesis Through ASXL2–EZH2–Mediated Microtubule Destabilization

Advanced Science, EarlyView.
This study reveals the mechanism by which chronic hypoxia impairs spermatogenesis via the ASXL2–EZH2 axis, hindering the transition of spermatids from round to elongated forms. Key findings reveal that under hypoxic conditions, downregulated ASXL2 expression reduces EZH2 binding to the CEP162 promoter, leading to decreased H3K27me3 modification and ...
Jun Yin, Mengjie Zhang, Wenying Liu, Wenlong Shen, Debao Li, Hongming Miao, Fang Deng, Gang Zhang, Yi Tian, Yi Zhang, Zhihu Zhao, Bing Ni   +11 more
wiley   +1 more source

The functional and catalytic landscape of urease reveals a conserved target against <i>Helicobacter pylori</i>. [PDF]

Gut Microbes
Song Q, Wu H, Ma Z, Huang T, Zhu X, Zhang Z, Wu G, Manzoor R, Liu S, Wang Y, Li X, Zhang W, Ye X, Ma H.   +13 more
europepmc   +1 more source