Results 261 to 270 of about 806,287 (306)

Synthetic promoter libraries – tuning of gene expression

Trends in Biotechnology, 2006
The study of gene function often requires changing the expression of a gene and evaluating the consequences. In principle, the expression of any given gene can be modulated in a quasi-continuum of discrete expression levels but the traditional approaches are usually limited to two extremes: gene knockout and strong overexpression. However, applications
Karin, Hammer, Ivan, Mijakovic, Peter Ruhdal, Jensen   +2 more
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Construction of gene libraries for each human chromosome

Cytometry, 1990
AbstractWe describe the construction of two complete sets of small insert, complete digest DNA libraries for each of the 24 human chromosomal types by the National Laboratory Gene Library Project. Flow sorting was used to purify the chromosomes which provided the DNA for cloning.
M A, Van Dilla, L L, Deaven
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A vector for construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae

Plasmid, 1987
We have constructed a convenient new vector, YEp-DE, for the construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae. The vector contains the yeast LEU2 gene, the 2 mu origin of replication, and a region from pUC18 that includes the ampr gene, the Escherichia coli origin of replication (ori), and the LacZ ...
M I, Khan, D J, Ecker, T, Butt, J A, Gorman, S T, Crooke   +4 more
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Rational design of orthogonal libraries of protein coding genes

Proceedings of the ACM Conference on Bioinformatics, Computational Biology and Biomedicine, 2012
Array-based oligonucleotide synthesis technologies provide access to thousands of custom-designed sequence variants at low cost. Large-scale synthesis and high-throughput assays have become valuable experimental tools to study in detail the interplay between sequence and function.
Daniel Ryan, Dimitris Papamichail
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Synthetic gene libraries: in search of the optimal diversity

Trends in Biotechnology, 2003
Directed evolution has proven to be an effective method for evolving proteins with desired properties. A key step is the creation of suitably diverse gene libraries. Two new methods for creating such libraries make sole use of synthesized oligonucleotides and allow researchers to tailor the diversity of a library with greater precision and create ...
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cDNA Libraries for Virus-Induced Gene Silencing

2010
Virus-induced gene silencing (VIGS) exploits endogenous plant antiviral defense mechanisms to posttranscriptionally silence the expression of targeted plant genes. VIGS is quick and relatively easy to perform and therefore serves as a powerful tool for high-throughput functional genomics in plants.
Andrea T, Todd, Enwu, Liu, Jonathan E, Page   +2 more
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Construction of Human Naïve Antibody Gene Libraries

2018
Size and variability often represent an obstacle in generating an effective antibody gene library for the detection of an abundant repertoire of antigens. Therefore, optimizing the construction of a large library is essential for the selection of high-affinity reactive fragments. Here, we report a highly efficient method for the construction of a human
Michela, Pasello, Alessandra, Mallano, Michela, Flego, Silvia, Zamboni, Anna Maria, Giudice, Katia, Scotlandi   +5 more
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[The library of Rickettsia prowazekii genes].

Molekuliarnaia genetika, mikrobiologiia i virusologiia, 1987
The DNA of Rickettsia provazekii strain E was cleaved by PstI restriction endonuclease under the conditions of partial restriction. The fragments were inserted into the PstI site of pBR325 and cloned in this plasmid. E. coli strain HB101 was used as a recipient for cloning.
M I, Artem'ev, E B, Rydkina, N M, Balaeva   +2 more
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Plant Genomic Sequencing Using Gene‐Enriched Libraries

ChemInform, 2007
AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
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Gene Library

2008
Stewart B. Peck, Carol C. Mapes, Netta Dorchin, John B. Heppner, Eileen A. Buss, Gustavo Moya-Raygoza, Marjorie A. Hoy, Gerald Franz, Alfred M. Handler, Marjorie A. Hoy, John L. Capinera, John B. Heppner, Bella R. Striganova, John B. Heppner, John B. Heppner, John B. Heppner, John B. Heppner, Marta Goula, Tobin D. Northfield, Russell F. Mizell, John B. Heppner, John B. Heppner, John B. Heppner, John B. Heppner, Rami Kfir, William A. Overholt, Des E. Conlong, Mamoudou Sétamou, Fritz Schulthess, William A. Overholt, Rami Kfir, Kitty F. Cardwell, Fritz Schulthess, Rami Kfir, Zeyaur R. Khan, William A. Overholt, Régis Goebel, Kia Rashidan, Claude Guertin, Jean Cabana, Doug Downie, George M. Orphanides, John L. Capinera, John L. Capinera, John L. Capinera, Steven Arthurs, John L. Capinera, Norma E. Sánchez, María L. De Wysiecki, John B. Heppner, Yousif Aldryhim, James C. Dunford, Kelly R. Sims, John L. Capinera, John L. Capinera, Gregory A. Sword, Eric W. Riddick, Paul M. Choate, Wayne Brewer   +58 more
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