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Gene discovery by ribozyme and siRNA libraries
Nature Reviews Molecular Cell Biology, 2005Catalytic RNAs, also known as ribozymes, can be engineered to optimize their activities in the intracellular environment. The introduction of a library of active ribozymes into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.
Hideo, Akashi +2 more
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Stalking the Gene -- DNA Libraries
New England Journal of Medicine, 1994For molecular biologists in search of clues to cell function, the ultimate object of inquiry is the gene. A scientist looking for a particular gene faces a formidable challenge, because each cell contains more than 100,000 different genes scattered over billions of nucleotides.
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Construction of Human Naive Antibody Gene Libraries
2012Human antibodies are valuable tools for proteome research and diagnostics. Furthermore, antibodies are a rapidly growing class of therapeutic agents, mainly for inflammation and cancer therapy. The first therapeutic antibodies are of murine origin and were chimerized or humanized. The later-developed antibodies are fully human antibodies.
Michael, Hust +4 more
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Gene Libraries: Coverage, Efficiency and Diversity
2006Gene libraries are a biological mechanism for generating combinatorial diversity in the immune system. However, they also bias the antibody creation process, so that they can be viewed as a way of guiding lifetime learning mechanisms. In this paper we examine the implications of this view, by examining coverage, avoidance of self, clustering and ...
Steve Cayzer, Jim Smith 0002
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Gene enrichment in plant genomic shotgun libraries
Current Opinion in Plant Biology, 2003The Arabidopsis genome (about 130 Mbp) has been completely sequenced; whereas a draft sequence of the rice genome (about 430 Mbp) is now available and the sequencing of this genome will be completed in the near future. The much larger genomes of several important crop species, such as wheat (about 16,000 Mbp) or maize (about 2500 Mbp), may not be fully
Pablo D, Rabinowicz +2 more
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Removing the Redundancy From Randomised Gene Libraries
Journal of Molecular Biology, 2003Amino acid substitution plays a vital role in both the molecular engineering of proteins and analysis of structure-activity relationships. High-throughput substitution is achieved by codon randomisation, which generates a library of mutants (a randomised gene library) in a single experiment.
Marcus D, Hughes +4 more
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Molekuliarnaia biologiia, 1990
Main approaches and methods used for constructing "jamping" and cDNA libraries, including novel ones, that omit the employment of methylases and linkers, are presented. The advantages and drawbacks of the well-known and new lambda vectors, suitable for the purposes mentioned, are discussed.
E R, Zabarovskiĭ, R L, Allikmets
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Main approaches and methods used for constructing "jamping" and cDNA libraries, including novel ones, that omit the employment of methylases and linkers, are presented. The advantages and drawbacks of the well-known and new lambda vectors, suitable for the purposes mentioned, are discussed.
E R, Zabarovskiĭ, R L, Allikmets
openaire +1 more source