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PotatoRTD and TomatoRTD: Comprehensive Reference Transcript Datasets for Accurate Transcriptome Analysis and Isoform Discovery. [PDF]

Sci Data
Guo W, Milne L, Lopez-Gomollon S, Kaur A, Hein I, Baulcombe D, Zhang R.   +6 more
europepmc   +1 more source

Primary transcript enrichment reveals a density-dependent <i>Wolbachia</i> transcriptome and active mosquito RNA viruses. [PDF]

Front Microbiol
Sinha A, Carlow CKS, Li Z.
europepmc   +1 more source

Genome-wide engineering for synthetic auxotrophy biocontainment in yeast

Cai Y, Hoffmann S, Miller T, Leeming D, McCullough M, Wan R, Leland B.   +6 more
europepmc   +1 more source

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Gene discovery by ribozyme and siRNA libraries

Nature Reviews Molecular Cell Biology, 2005
Catalytic RNAs, also known as ribozymes, can be engineered to optimize their activities in the intracellular environment. The introduction of a library of active ribozymes into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.
Hideo, Akashi, Sahohime, Matsumoto, Kazunari, Taira   +2 more
openaire   +4 more sources

Stalking the Gene -- DNA Libraries

New England Journal of Medicine, 1994
For molecular biologists in search of clues to cell function, the ultimate object of inquiry is the gene. A scientist looking for a particular gene faces a formidable challenge, because each cell contains more than 100,000 different genes scattered over billions of nucleotides.
openaire   +2 more sources

Gene Libraries: Coverage, Efficiency and Diversity

2006
Gene libraries are a biological mechanism for generating combinatorial diversity in the immune system. However, they also bias the antibody creation process, so that they can be viewed as a way of guiding lifetime learning mechanisms. In this paper we examine the implications of this view, by examining coverage, avoidance of self, clustering and ...
Steve Cayzer, Jim Smith 0002
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Construction of Human Naive Antibody Gene Libraries

2012
Human antibodies are valuable tools for proteome research and diagnostics. Furthermore, antibodies are a rapidly growing class of therapeutic agents, mainly for inflammation and cancer therapy. The first therapeutic antibodies are of murine origin and were chimerized or humanized. The later-developed antibodies are fully human antibodies.
Michael, Hust, André, Frenzel, Torsten, Meyer, Thomas, Schirrmann, Stefan, Dübel   +4 more
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[Construction of libraries of structural genes (cDNA) and "jumping" gene libraries in lambda vectors].

Molekuliarnaia biologiia, 1990
Main approaches and methods used for constructing "jamping" and cDNA libraries, including novel ones, that omit the employment of methylases and linkers, are presented. The advantages and drawbacks of the well-known and new lambda vectors, suitable for the purposes mentioned, are discussed.
E R, Zabarovskiĭ, R L, Allikmets
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