Results 61 to 70 of about 27,029,195 (311)

Kaufman Library Faculty/Staff Newsletter, Fall 2016

, 2016
Fall 2016 issue of the Georgia Gwinnett College Daniel J. Kaufman Library & Learning Center newsletter for faculty and staff. 7 pages. Contents: "Library Dedication", "Quiet Reading Room Exhibit", "Research Services and the Information Literacy Omnibus",
Kaufman Library, GGC Library
core  

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Construction of Gene Targeting Vectors from λKOS Genomic Libraries

BioTechniques, 1999
We describe a highly redundant murine genomic library in a new λ phage, λ knockout shuttle (λKOS) that facilitates the very rapid construction of replacement-type gene targeting vectors.
Sigrid Wattler, Mike Kelly, Michael Nehls   +2 more
doaj   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Construction of the suppression subtractive cDNA libraries of human large cell lung cancer line L9981 before and after transfection with nm23-H1 gene

Chinese Journal of Lung Cancer, 2008
Background and objective It has been proven that nm23-H1 gene is an important metastatic-suppressed gene of lung cancer. In order to screen the differential expression genes related to nm23-H1, we constructed the suppression subtractive cDNA libraries of
Sujuan YE, Zhihua FENG, Wen ZHU, Chunji CAI, Lu LI, Liya SUN, Haisu WAN, Li MA, Qinghua ZHOU   +8 more
doaj  

Chromosome-segment scanning for gain- or loss-of-function screening (CHASING)

iScience
Summary: Identifying beneficial or functional mutations for a specific species is a task always relies on the labor-intensive construction of a library containing thousands of single-gene knockout or interference strains.
Yan Xia, Lichao Sun, Zeyu Liang, Zhongrao Han, Jing Li, Pengyu Dong, Yi-Xin Huo, Shuyuan Guo   +7 more
doaj   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Randomly barcoded transposon mutant libraries for gut commensals I: Strategies for efficient library construction

Cell Reports
Summary: Randomly barcoded transposon mutant libraries are powerful tools for studying gene function and organization, assessing gene essentiality and pathways, discovering potential therapeutic targets, and understanding the physiology of gut bacteria ...
Surya Tripathi, Carlos Geert Pieter Voogdt, Stefan Oliver Bassler, Mary Anderson, Po-Hsun Huang, Nazgul Sakenova, Tümay Capraz, Sunit Jain, Alexandra Koumoutsi, Afonso Martins Bravo, Valentine Trotter, Michael Zimmerman, Justin L. Sonnenburg, Cullen Buie, Athanasios Typas, Adam M. Deutschbauer, Anthony L. Shiver, Kerwyn Casey Huang   +17 more
doaj   +1 more source

Fosmid library construction and screening for the maize mutant gene Vestigial glume 1

Crop Journal, 2016
The maize mutant gene Vestigial glume 1 (Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening.
Chaoxian Liu, Xiaoli Liu, Lei Lei, Haiying Guan, Yilin Cai   +4 more
doaj   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source