Results 81 to 90 of about 1,333,192 (304)

Gene and library synthesis without amplification: polymerase step reaction (PSR)

BioTechniques, 2015
Current gene synthesis methods often incorporate a PCR amplification step in order to yield final material sufficient for resolution from multiple off-products. These amplification steps can cause stochastic sampling effects that propagate errors in gene
Zhuo-Bin Lee, Christopher Firnhaber, Jesse Clarke, Brian S. DeDecker   +3 more
doaj   +1 more source

Response to fungal exudates of the rhizosphere isolate Pseudomonas sp. UMAF110 involves a GGDEF/EAL domain-containing protein [PDF]

, 2017
Pseudomonas sp. UMAF110, isolated from rhizosphere soil in Spain, display in vitro antagonism towards the pythopathogenic fungus Rosellinia necatrix and is able grow in fungal exudates (BM-RE medium).
Aragón, Isabel María, Cazorla-Lopez, Francisco Manuel, Crespo Gómez, J. Ignacio, De-Vicente-Moreno, Antonio, Pintado, Adrian, Pliego, Clara, Pérez-Martínez, Isabel, Ramos, Cayo   +7 more
core  

RNA-Seq optimization with eQTL gold standards. [PDF]

, 2013
BackgroundRNA-Sequencing (RNA-Seq) experiments have been optimized for library preparation, mapping, and gene expression estimation. These methods, however, have revealed weaknesses in the next stages of analysis of differential expression, with results ...
Arking, Dan E, Ashar, Foram N, Bader, Joel S, Ellis, Shannon E, Gupta, Simone, West, Andrew B   +5 more
core   +2 more sources

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Chromosome-segment scanning for gain- or loss-of-function screening (CHASING)

iScience
Summary: Identifying beneficial or functional mutations for a specific species is a task always relies on the labor-intensive construction of a library containing thousands of single-gene knockout or interference strains.
Yan Xia, Lichao Sun, Zeyu Liang, Zhongrao Han, Jing Li, Pengyu Dong, Yi-Xin Huo, Shuyuan Guo   +7 more
doaj   +1 more source

cDNA and Gene Analyses Imply a Novel Structure for a Rat Carcinoembryonic Antigen-related Protein [PDF]

, 1990
The gene encoding the human tumor marker carcinoembryonic antigen (CEA) belongs to a gene family which can be subdivided into the CEA and the pregnancy-specific glycoprotein subgroups.
Lucas, Kurt, Rebstock, Sabine, Thompson, John A., Zimmermann, Wolfgang   +3 more
core  

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source

Construction of the suppression subtractive cDNA libraries of human large cell lung cancer line L9981 before and after transfection with nm23-H1 gene

Chinese Journal of Lung Cancer, 2008
Background and objective It has been proven that nm23-H1 gene is an important metastatic-suppressed gene of lung cancer. In order to screen the differential expression genes related to nm23-H1, we constructed the suppression subtractive cDNA libraries of
Sujuan YE, Zhihua FENG, Wen ZHU, Chunji CAI, Lu LI, Liya SUN, Haisu WAN, Li MA, Qinghua ZHOU   +8 more
doaj  

KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer

Molecular Oncology, EarlyView.
Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan, Veronika M Metzler, Simone de Brot, Corinne L Woodcock, Anna E Harris, Jennifer Lothion‐Roy, Emeli M Nilsson, Atara Ntekim, Michael S Toss, Jenny L Persson, Francesca Khani, Brian D Robinson, Lorraine J Gudas, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan   +16 more
wiley   +1 more source

Randomly barcoded transposon mutant libraries for gut commensals I: Strategies for efficient library construction

Cell Reports
Summary: Randomly barcoded transposon mutant libraries are powerful tools for studying gene function and organization, assessing gene essentiality and pathways, discovering potential therapeutic targets, and understanding the physiology of gut bacteria ...
Surya Tripathi, Carlos Geert Pieter Voogdt, Stefan Oliver Bassler, Mary Anderson, Po-Hsun Huang, Nazgul Sakenova, Tümay Capraz, Sunit Jain, Alexandra Koumoutsi, Afonso Martins Bravo, Valentine Trotter, Michael Zimmerman, Justin L. Sonnenburg, Cullen Buie, Athanasios Typas, Adam M. Deutschbauer, Anthony L. Shiver, Kerwyn Casey Huang   +17 more
doaj   +1 more source