Creating a RAW264.7 CRISPR-Cas9 Genome Wide Library
Bio-Protocol, 2017 The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome editing tools are used in mammalian cells to knock-out specific genes of interest to elucidate gene function.
Brooke Napier , Denise Monack
doaj +1 more source
KNR4, a suppressor of Saccharomyces cerevisiae cwh mutants, is involved in the transcriptional control of chitin synthase genes [PDF]
, 1999 The KNR4 gene, originally isolated by complementation of a K9 killer-toxinresistant mutant displaying reduced levels of both 1,3-b-glucan and 1,3-bglucan synthase activity, was recloned from a YCp50 genomic library as a suppressor of Saccharomyces ...
Dagkessamanskaia, Adilia +4 more
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Molecular Oncology, EarlyView.CIN85 is highly expressed in osteosarcoma, particularly in metastatic lesions. Its overexpression increases cell migration and Matrigel invasion, while silencing CIN85 suppresses these behaviors. Transcriptome analysis shows that CIN85 regulates MMP2, COL3A1, and Akt/mTOR signaling. Targeting these pathways reverses CIN85‐induced motility, highlighting
Iryna Horak +10 more
wiley +1 more source
Development of an efficient transformation system for Dothistromin pini : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University [PDF]
, 1996 A transformation system has been developed for the plant pathogenic fungus Dothistroma pini using a positive selection system based on the Escherichia coli hygromycin B phosphotransferase gene (hph).
Bidlake, Anita Therese
core
Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources
Molecular Oncology, EarlyView.Intratumour heterogeneity complicates precision management of advanced endometrial cancer. Circulating tumor DNA (ctDNA) offers a minimally invasive strategy to capture tumor evolution and therapeutic resistance. Here, we compare tumor‐agnostic NGS with tumor‐informed ddPCR, outlining their relative sensitivity, concordance, and clinical implications ...
Carlos Casas‐Arozamena +15 more
wiley +1 more source
Fosmid library construction and screening for the maize mutant gene Vestigial glume 1
Crop Journal, 2016 The maize mutant gene Vestigial glume 1 (Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening.
Chaoxian Liu +4 more
doaj +1 more source
Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in Stenotrophomonas maltophilia and Chryseobacterium spp. Bacteria of Soil Origin. [PDF]
Int J Mol Sci, 2023 Klimkaitė L +5 more
europepmc +1 more source
Identification of a CD4+ T cell-stimulating antigen of pathogenic bacteria by expression cloning. [PDF]
, 1995 Identifying the immunogenic proteins that elicit pathogen-specific T cell responses is key to rational vaccine design. While several approaches have succeeded in identifying major histocompatibility complex (MHC) class I bound peptides that stimulate CD8+
Campbell, D +2 more
core +1 more source
Molecular Oncology, EarlyView.A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel +12 more
wiley +1 more source