Results 91 to 100 of about 806,287 (306)

Shovel, groundbreaking ceremony for the Roseland Free Public Library, Roseland, N.J., November 17, 1985

open access: yes, 1985
Shovel marks the spot. Gold shovel, that is! Photo of the first shovel and stake in the ground of the occasion of the Groundbreaking Ceremony of the new library site, 20 Roseland Avenue, Roseland, NJ, on Sunday, November 17 ...

core  

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Reliability analysis of the Ahringer Caenorhabditis elegans RNAi feeding library: a guide for genome-wide screens

open access: yesBMC Genomics, 2011
Background The Ahringer C. elegans RNAi feeding library prepared by cloning genomic DNA fragments has been widely used in genome-wide analysis of gene function.
Lu Yiming   +9 more
doaj   +1 more source

Integrative annotation of 21,037 human genes validated by full-length cDNA clones

open access: yes, 2004
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited.
Terwilliger Joseph D   +682 more
core   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

An improved method of constructing degradome library suitable for sequencing using Illumina platform

open access: yesPlant Methods, 2019
Background Post-transcriptional gene regulation is one of the critical layers of overall gene expression programs and microRNAs (miRNAs) play an indispensable role in this process by guiding cleavage on the messenger RNA targets.
Yong-Fang Li   +8 more
doaj   +1 more source

The National Laboratory Gene Library Project [PDF]

open access: yes, 1989
The two National Laboratories at Livermore and Los Alamos have played a prominent role in the development and application of flow cytometry and sorting to chromosome classification and purification. Both laboratories began to receive numerous requests for specific human chromosomal types purified by flow sorting for gene library construction, but these
M.A. VAN DILLA   +35 more
openaire   +1 more source

Interview with Gene Doughty

open access: yes, 2005
Sergeant Gene Doughty, born in Stamford, Connecticut, moved to New York City, where he finished high school and entered City College. He joined the Corps in 1943 and participated in the invasion of Iwo Jima.
Doughty, Gene
core  

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

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