Results 111 to 120 of about 806,287 (306)

Large‐scale image‐based profiling of single‐cell phenotypes in arrayed CRISPR‐Cas9 gene perturbation screens

Molecular Systems Biology, 2018
High‐content imaging using automated microscopy and computer vision allows multivariate profiling of single‐cell phenotypes. Here, we present methods for the application of the CISPR‐Cas9 system in large‐scale, image‐based, gene perturbation experiments.
Reinoud de Groot, Joel Lüthi, Helen Lindsay, René Holtackers, Lucas Pelkmans   +4 more
doaj   +1 more source

Library Services to Canadian Native People

, 1980
A paper written by Xwi7xwa Library founding librarian, Gene Joseph, while studying at the University of British Columbia's School of Library, Archival, and Information Studies (SLAIS). The paper discusses issues concerning library services for Aboriginal
Joseph, Gene
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Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum.

, 2010
Yang I, John U, Beszteri S, et al. Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum. BMC Genomics.
Uwe John, Glöckner, Gernot, Allan D Cembella, John, Uwe, Beszteri, Sara, Goesmann, Alexander ; https://orcid.org/, Ines Yang, Gernot Glöckner, Krock, Bernd, Gloeckner, G., Goesmann, A., Sára Beszteri, Cembella, Allan, Yang, Ines, Cembella, Allan D., Bernd Krock, Alexander Goesmann   +16 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

GRiNS: a python library for simulating gene regulatory network dynamics

BMC Bioinformatics
Background The emergent dynamics of complex gene regulatory networks govern various cellular processes. However, understanding these dynamics is challenging due to the difficulty of parameterizing the computational models for these networks, especially ...
Pradyumna Harlapur, Harshavardhan BV, Mohit Kumar Jolly   +2 more
doaj   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

FEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin, Ziyang Yuan, Wenli Jiang, Ai Guo   +3 more
wiley   +1 more source

Gene Cloning and Recombinant DNA Libraries

, 2023
Gene cloning involves the replication of DNA fragments in host organisms to produce identical copies. It serves as a vital tool for the production of proteins, vaccines, and therapeutic treatments. On the other hand, rDNA libraries—systematic collections of cloned DNA sequences—function as extensive genomic and cDNA archives.
openaire   +1 more source

Richard Leonard, Mayor, speech, groundbreaking ceremony for the Roseland Free Public Library, Roseland, N.J., November 17, 1985

, 1985
The Mayor speaks. Photo of the Mayor's speech to the guests of the Groundbreaking Ceremony at the new library site, 20 Roseland Avenue, Roseland, NJ, Sunday, November 17 ...

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Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

World 2014

, 2014
"The Cahill-Keyes projection is a polyhedral compromise map projection first proposed by Gene Keyes in 1975. The projection is a refinement of an earlier 1909 projection by Cahill.
Keyes, Gene;
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