Results 101 to 110 of about 1,333,192 (304)

A cDNA library of the eutardigrade Hypsibius klebelsbergi Mihelčič, 1959 and analysis of the actin gene

open access: yesJournal of Limnology, 2007
A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN   +3 more
doaj   +1 more source

A Synthetic Gene Library Yields a Previously Unknown Glycoside Phosphorylase That Degrades and Assembles Poly-β-1,3-GlcNAc, Completing the Suite of β-Linked GlcNAc Polysaccharides. [PDF]

open access: yesACS Cent Sci, 2022
Macdonald SS   +9 more
europepmc   +1 more source

Transcriptome Analyses of Tumor-Adjacent Somatic Tissues Reveal Genes Co-Expressed with Transposable Elements [PDF]

open access: yes, 2019
Background: Despite the long-held assumption that transposons are normally only expressed in the germ-line, recent evidence shows that transcripts of transposable element (TE) sequences are frequently found in the somatic cells.
Alberto, Adrian   +9 more
core   +2 more sources

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Selection of an Efficient AAV Vector for Robust CNS Transgene Expression

open access: yesMolecular Therapy: Methods & Clinical Development, 2019
Adeno-associated virus (AAV) capsid libraries have generated improved transgene delivery vectors. We designed an AAV library construct, iTransduce, that combines a peptide library on the AAV9 capsid with a Cre cassette to enable sensitive detection of ...
Killian S. Hanlon   +15 more
doaj   +1 more source

Faculty recital series: John Muratore, guitar with Roberto Cassan, accordion, November 8, 2007 [PDF]

open access: yes, 2007
This is the concert program of the Faculty recital series: John Muratore, guitar with Roberto Cassan, accordion performance on Thursday, November 8, 2007 at 8:00 p.m., at the Tsai Performance Center, 685 Commonwealth Avenue.
School of Music, Boston University
core  

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Reliability analysis of the Ahringer Caenorhabditis elegans RNAi feeding library: a guide for genome-wide screens

open access: yesBMC Genomics, 2011
Background The Ahringer C. elegans RNAi feeding library prepared by cloning genomic DNA fragments has been widely used in genome-wide analysis of gene function.
Lu Yiming   +9 more
doaj   +1 more source

Identification of dothistromin biosynthetic pathway genes : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University, Palmerston North, New Zealand [PDF]

open access: yes, 1996
Dothistromin is a polyketide-derived toxic secondary metabolite produced by the filamentous fungus Dothistroma pini which causes the disease Dothistroma needle blight in Pinus radiata.
Gillman, Carmel Jane
core  

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

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