Molecular characterization of mecA gene in Methicillin-Resistant Staphylococcus aureus
Background & objectives: It is clear now that methicillin-resistant Staphylococcus aureus (MRSA) strains considered as one of the most bacteria responsible for different diseases among humans and animals.
Saad Salman Hamim
doaj +7 more sources
Introduction: Staphylococci are common human commensals that acquire methicillin resistance via the mecA gene. Methicillin resistance in staphylococci from various clinical sources has been assessed using cefoxitin disc diffusion test (CDDT) and PCR ...
WMDA Wijesundara +3 more
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Coagulase-negative staphylococci (CNS), in particular Staphylococcus haemolyticus, play an important role in the etiology of nosocomial infections. Most CNS are resistant to beta-lactam antibiotics, which is realized through the production of the second ...
A. V. Polonskaya +8 more
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Screening for mecA and mecC Gene Carriage among Clinical Isolates of Methicillin Resistant Staphylococcus aureus at a Tertiary Care Hospital: A Cross-sectional Study [PDF]
Introduction: Staphylococcus aureus (S. aureus) has emerged as one of the most important human pathogen, and has been a leading cause of hospital and community acquired infections. Methicillin-Resistant Staphylococcus aureus (MRSA) carrying the mecA gene
Rakshita Ramesh +3 more
doaj +1 more source
Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals [PDF]
In this work there was evaluated the method of detection of methicillin resistant Staphylococcus aureus (MRSA) by using two molecular and three phenotypic tests in investigation procedure of 70 strains of S.aureus isolated from animals.
Radosavljević V. +5 more
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Guidelines for Reporting Novel mecA Gene Homologues [PDF]
Methicillin-resistant staphylococci are disseminated all over the world and are frequent causes of health care- and community-associated infections. Methicillin-resistant strains typically carry the acquired mecA gene that encodes a low-affinity penicillin-binding protein (PBP), designated PBP2a ...
Ito, T. +25 more
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Detection of the Staphylococcal mecA gene by chemiluminescent DNA hybridization [PDF]
A new solution-phase DNA hybridization capture assay for the rapid detection of the mecA gene in clinical isolates of Staphylococcus was compared with multiplex PCR and disk diffusion methods. The assay uses a DNA capture probe immobilized on paramagnetic particles and a second DNA probe labeled with an acridinium ester. Bacteria from 24-h cultures are
C P, Kolbert +3 more
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A potential role of transposon IS431 in the loss of mecA gene [PDF]
AbstractAcquisition of a vancomycin-resistance-determinant may trigger deletion of the mecA gene. However, the molecular mechanisms involved remain largely unknown. In this study, we successfully produced vancomycin-intermediate-resistant Staphylococcus aureus (VISA) from Methicillin-resistant-S.
Aihua Wang +7 more
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Primer Design and Analysis for Detection of mecA gene
MecA is a gene that causes antibiotic resistance and it contained in Staphylococcus aureus. The gene can be detected using pairs of primer (forward and reverse). Primes is short nucleotide that are used as attachment point for DNA polymerase and as a barrier for the fragment DNA target to be amplified with Polymerase Chain Reaction (PCR).
Armini Syamsidi +3 more
openaire +3 more sources
Molecular and phylogenetic study of Staphylococcus aureus isolated from human and cattle of Al-Qadisiyah Governorate, Iraq [PDF]
Aim: This study was designed to detect the prevalence of Staphylococcus aureus, to estimate the frequency of methicillin resistance gene (mecA), femA (specific gene for S.
Ahmed Jasim Neamah +4 more
doaj +1 more source

